Reviewed, UniProtKB/Swiss-Prot P65257 (LDH1_STAAW)

Names and origin

Protein names

Recommended name:
    L-lactate dehydrogenase 1
      Short name=L-LDH 1
    EC=1.1.1.27

Gene names

Name:	ldhA
Synonyms:	lctE
Ordered Locus Names:	MW0217

Organism

Staphylococcus aureus (strain MW2) [Complete proteome] [HAMAP]

Taxonomic identifier

196620 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Appears to be the primary factor that allows S.aureus growth during nitrosative stress in both aerobically and anaerobically cultured cells By similarity. HAMAP MF_00488
Catalytic activity	(S)-lactate + NAD+ = pyruvate + NADH. HAMAP MF_00488
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. HAMAP MF_00488
Subunit structure	Homotetramer By similarity. HAMAP MF_00488
Subcellular location	Cytoplasm By similarity HAMAP MF_00488.
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
Biological process	Glycolysis Stress response
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	anaerobic glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	L-lactate dehydrogenase 1 HAMAP MF_00488		PRO_0000168387
Regions
	Nucleotide binding	15 – 43	29	NAD By similarity
Sites
	Active site	179	1	Proton acceptor By similarity
	Binding site	92	1	Substrate By similarity
	Binding site	124	1	NAD or substrate By similarity
	Binding site	155	1	Substrate By similarity
	Binding site	232	1	Substrate By similarity
Amino acid modifications
	Modified residue	223	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P65257-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: CDDE4813AF6CB226	FASTA	317	34,569
	10 20 30 40 50 60 MNKFKGNKVV LIGNGAVGSS YAFSLVNQSI VDELVIIDLD TEKVRGDVMD LKHATPYSPT 70 80 90 100 110 120 TVRVKAGEYS DCHDADLVVI CAGAAQKPGE TRLDLVSKNL KIFKSIVGEV MASKFDGIFL 130 140 150 160 170 180 VATNPVDILA YATWKFSGLP KERVIGSGTI LDSARFRLLL SEAFDVAPRS VDAQIIGEHG 190 200 210 220 230 240 DTELPVWSHA NIAGQPLKTL LEQRPEGKAQ IEQIFVQTRD AAYDIIQAKG ATYYGVAMGL 250 260 270 280 290 300 ARITEAIFRN EDAVLTVSAL LEGEYDEEDV YIGVPAVINR NGIRNVVEIP LNDEEQSKFA 310 HSAKTLKDIM AEAEELK

References

[1]

"Genome and virulence determinants of high virulence community-acquired MRSA." Baba T., Takeuchi F., Kuroda M., Yuzawa H., Aoki K., Oguchi A., Nagai Y., Iwama N., Asano K., Naimi T., Kuroda H., Cui L., Yamamoto K., Hiramatsu K. Lancet 359:1819-1827(2002) [PubMed: 12044378] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000033 Genomic DNA. Translation: BAB94082.1.
RefSeq	NP_645032.1.
3D structure databases
SMR	P65257. Positions 1-313.
ModBase	Search...
Protein-protein interaction databases
STRING	P65257.
Genome annotation databases
GeneID	1004969.
GenomeReviews	Gene locus MW0217 in contig BA000033_GR.
KEGG	sam:MW0217.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HBG566126.
OMA	CAGANQK.
Enzyme and pathway databases
BioCyc	SAUR196620:MW0217-MONOMER.
Family and domain databases
HAMAP	MF_00488. Lactate_dehydrog. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR011304. L-lactate_DH. IPR018177. L-lactate_DH_AS. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDH1_STAAW

Accession

Primary (citable) accession number: P65257
Secondary accession number(s): Q99WY2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	February 9, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)