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Protein

L-lactate dehydrogenase 1

Gene

ldhA

Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Appears to be the primary factor that allows S.aureus growth during nitrosative stress in both aerobically and anaerobically cultured cells.By similarity

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase 2 (ldhB), L-lactate dehydrogenase 1 (ldhA)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei92SubstrateBy similarity1
Binding sitei124NAD or substrateBy similarity1
Binding sitei155SubstrateBy similarity1
Active sitei179Proton acceptorBy similarity1
Binding sitei232SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 43NADBy similarityAdd BLAST29

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processStress response
LigandNAD

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase 1 (EC:1.1.1.27)
Short name:
L-LDH 1
Gene namesi
Name:ldhA
Synonyms:lctE
Ordered Locus Names:SAV0241
OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
Taxonomic identifieri158878 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000002481 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001683811 – 317L-lactate dehydrogenase 1Add BLAST317

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei223PhosphotyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP65255
PRIDEiP65255

2D gel databases

World-2DPAGEi0002:P65255

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi158878.SAV0241

Structurei

3D structure databases

ProteinModelPortaliP65255
SMRiP65255
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiENOG4105C80 Bacteria
COG0039 LUCA
HOGENOMiHOG000213793
KOiK00016
OMAiMDLMQTA
PhylomeDBiP65255

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_00488 Lactate_dehydrog, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR011304 L-lactate_DH
IPR018177 L-lactate_DH_AS
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
PRINTSiPR00086 LLDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01771 L-LDH-NAD, 1 hit
PROSITEiView protein in PROSITE
PS00064 L_LDH, 1 hit

Sequencei

Sequence statusi: Complete.

P65255-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKFKGNKVV LIGNGAVGSS YAFSLVNQSI VDELVIIDLD TEKVRGDVMD
60 70 80 90 100
LKHATPYSPT TVRVKAGEYS DCHDADLVVI CAGAAQKPGE TRLDLVSKNL
110 120 130 140 150
KIFKSIVGEV MASKFDGIFL VATNPVDILA YATWKFSGLP KERVIGSGTI
160 170 180 190 200
LDSARFRLLL SEAFDVAPRS VDAQIIGEHG DTELPVWSHA NIAGQPLKTL
210 220 230 240 250
LEQRPEGKAQ IEQIFVQTRD AAYDIIQAKG ATYYGVAMGL ARITEAIFRN
260 270 280 290 300
EDAVLTVSAL LEGEYDEEDV YIGVPAVINR NGIRNVVEIP LNDEEQSKFA
310
HSAKTLKDIM AEAEELK
Length:317
Mass (Da):34,569
Last modified:October 11, 2004 - v1
Checksum:iCDDE4813AF6CB226
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA Translation: BAB56403.1
RefSeqiWP_001031880.1, NC_002758.2

Genome annotation databases

EnsemblBacteriaiBAB56403; BAB56403; SAV0241
KEGGisav:SAV0241

Similar proteinsi

Entry informationi

Entry nameiLDH1_STAAM
AccessioniPrimary (citable) accession number: P65255
Secondary accession number(s): Q99WY2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: March 28, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health