Reviewed, UniProtKB/Swiss-Prot P65245 (KPRS1_STRP8)

Names and origin

Protein names

Recommended name:
    Ribose-phosphate pyrophosphokinase 1
      Short name=RPPK 1
    EC=2.7.6.1
Alternative name(s):
    Phosphoribosyl pyrophosphate synthetase 1
      Short name=P-Rib-PP synthetase 1
      Short name=PRPP synthetase 1

Gene names

Name:	prs1
Synonyms:	prsA, prsA.1
Ordered Locus Names:	spyM18_0021

Organism

Streptococcus pyogenes serotype M18 [Complete proteome] [HAMAP]

Taxonomic identifier

301451 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	Ribose-phosphate pyrophosphokinase 1 HAMAP MF_00583		PRO_0000141214
Regions
	Region	217 – 230	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	130	1	Magnesium Potential
	Metal binding	132	1	Magnesium Potential
	Metal binding	141	1	Magnesium Potential
	Metal binding	145	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	P65245-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 8DD79296FCABD695	FASTA	320	35,044
	10 20 30 40 50 60 MSYSDLKLFA LSSNKELAEK VASAMGIQLG KSTVRQFSDG EIQVNIEESI RGHHVFILQS 70 80 90 100 110 120 TSSPVNDNLM EILIMVDALK RASAEKISVV MPYYGYARQD RKARSREPIT SKLVANMLEV 130 140 150 160 170 180 AGVDRLLTVD LHAAQIQGFF DIPVDHLMGA PLIADYFDRH GLVGEDVVVV SPDHGGVTRA 190 200 210 220 230 240 RKLAQFLQTP IAIIDKRRSV DKMNTSEVMN IIGNVSGKKC ILIDDMIDTA GTICHAADAL 250 260 270 280 290 300 AEAGATAVYA SCTHPVLSGP ALDNIQRSAI EKLIVLDTIY LPKERLIDKI EQISIADLVA 310 320 EAIIRIHEKR PLSPLFEMGN

References

[1]

"Genome sequence and comparative microarray analysis of serotype M18 group A Streptococcus strains associated with acute rheumatic fever outbreaks." Smoot J.C., Barbian K.D., Van Gompel J.J., Smoot L.M., Chaussee M.S., Sylva G.L., Sturdevant D.E., Ricklefs S.M., Porcella S.F., Parkins L.D., Beres S.B., Campbell D.S., Smith T.M., Zhang Q., Kapur V., Daly J.A., Veasy L.G., Musser J.M. Proc. Natl. Acad. Sci. U.S.A. 99:4668-4673(2002) [PubMed: 11917108] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MGAS8232 / Serotype M18.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE009949 Genomic DNA. Translation: AAL96850.1.
RefSeq	NP_606351.1.
3D structure databases
HSSP	HSSP built from PDB template 1DKR based on UniProtKB P14193.
ModBase	Search...
Genome annotation databases
GeneID	995234.
GenomeReviews	Gene locus spyM18_0021 in contig AE009949_GR.
KEGG	spm:spyM18_0021.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P65245.
OMA	NVAEIMN.
Enzyme and pathway databases
BioCyc	SPYO186103:SPYM18_0021-MON.
Family and domain databases
HAMAP	MF_00583. [Tree]
InterPro	IPR000842. PRib-PP_synthetase_CS. IPR000836. PRibTrfase. IPR005946. PRPP_kinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. ribP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHETASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS1_STRP8

Accession

Primary (citable) accession number: P65245
Secondary accession number(s): Q9A1Z7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	November 3, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)