P65243 (KPRS1_STRP1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribose-phosphate pyrophosphokinase 1
Short name=RPPK 1
EC=2.7.6.1

Alternative name(s):
Phosphoribosyl pyrophosphate synthase 1
Short name=P-Rib-PP synthase 1
Short name=PRPP synthase 1

Gene names

Name:	prs1
Synonyms:	prsA, prsA.1
Ordered Locus Names:	SPy_0020, M5005_Spy0018

Organism

Streptococcus pyogenes serotype M1 [Complete proteome] [HAMAP]

Taxonomic identifier

301447 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583_B
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00583_B
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00583_B.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	Ribose-phosphate pyrophosphokinase 1 HAMAP MF_00583_B		PRO_0000141208
Regions
	Region	217 – 230	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	130	1	Magnesium Potential
	Metal binding	132	1	Magnesium Potential
	Metal binding	141	1	Magnesium Potential
	Metal binding	145	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	P65243 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 8DD79296FCABD695	FASTA	320	35,044
	10 20 30 40 50 60 MSYSDLKLFA LSSNKELAEK VASAMGIQLG KSTVRQFSDG EIQVNIEESI RGHHVFILQS 70 80 90 100 110 120 TSSPVNDNLM EILIMVDALK RASAEKISVV MPYYGYARQD RKARSREPIT SKLVANMLEV 130 140 150 160 170 180 AGVDRLLTVD LHAAQIQGFF DIPVDHLMGA PLIADYFDRH GLVGEDVVVV SPDHGGVTRA 190 200 210 220 230 240 RKLAQFLQTP IAIIDKRRSV DKMNTSEVMN IIGNVSGKKC ILIDDMIDTA GTICHAADAL 250 260 270 280 290 300 AEAGATAVYA SCTHPVLSGP ALDNIQRSAI EKLIVLDTIY LPKERLIDKI EQISIADLVA 310 320 EAIIRIHEKR PLSPLFEMGN

References

[1]	"Complete genome sequence of an M1 strain of Streptococcus pyogenes." Ferretti J.J., McShan W.M., Ajdic D.J., Savic D.J., Savic G., Lyon K., Primeaux C., Sezate S., Suvorov A.N., Kenton S., Lai H.S., Lin S.P., Qian Y., Jia H.G., Najar F.Z., Ren Q., Zhu H., Song L. , White J., Yuan X., Clifton S.W., Roe B.A., McLaughlin R.E. Proc. Natl. Acad. Sci. U.S.A. 98:4658-4663(2001) [PubMed: 11296296] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700294 / SF370 / Serotype M1.
[2]	"Evolutionary origin and emergence of a highly successful clone of serotype M1 group A Streptococcus involved multiple horizontal gene transfer events." Sumby P., Porcella S.F., Madrigal A.G., Barbian K.D., Virtaneva K., Ricklefs S.M., Sturdevant D.E., Graham M.R., Vuopio-Varkila J., Hoe N.P., Musser J.M. J. Infect. Dis. 192:771-782(2005) [PubMed: 16088826] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-947 / MGAS5005 / Serotype M1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004092 Genomic DNA. Translation: AAK33159.1. CP000017 Genomic DNA. Translation: AAZ50637.1.
RefSeq	NP_268437.1. NC_002737.1. YP_281382.1. NC_007297.1.
3D structure databases
ProteinModelPortal	P65243.
SMR	P65243. Positions 5-316.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000001106; EBSTRP00000001106; EBSTRG00000001106. EBSTRT00000028154; EBSTRP00000027172; EBSTRG00000028154.
GeneID	3572902. 900380.
GenomeReviews	Gene locus SPy_0020 in contig AE004092_GR. Gene locus M5005_Spy0018 in contig CP000017_GR.
KEGG	spy:SPy_0020. spz:M5005_Spy_0018.
PATRIC	19713854. VBIStrPyo79812_0019.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000027352.
HOGENOM	HBG519284.
OMA	CATHAVF.
ProtClustDB	PRK02269.
Enzyme and pathway databases
BioCyc	SPYO160490:SPY0020-MONOMER. SPYO293653:M5005_SPY0018-MONOMER.
Family and domain databases
HAMAP	MF_00583_B. RibP_PPkinase_B. [Tree]
InterPro	IPR000842. PRib_PP_synth_CS. IPR000836. PRibTrfase. IPR005946. Rib-P_diPkinase. [Graphical view]
KO	K00948.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. RibP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS1_STRP1

Accession

Primary (citable) accession number: P65243
Secondary accession number(s): Q491T1, Q9A1Z7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program