Reviewed, UniProtKB/Swiss-Prot P65240 (KPRS1_STRR6)

Names and origin

Protein names

Recommended name:
    Ribose-phosphate pyrophosphokinase 1
      Short name=RPPK 1
    EC=2.7.6.1
Alternative name(s):
    Phosphoribosyl pyrophosphate synthetase 1
      Short name=P-Rib-PP synthetase 1
      Short name=PRPP synthetase 1

Gene names

Name:	prs1
Synonyms:	prsA
Ordered Locus Names:	spr0028

Organism

Streptococcus pneumoniae (strain ATCC BAA-255 / R6) [Complete proteome] [HAMAP]

Taxonomic identifier

171101 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 322	322	Ribose-phosphate pyrophosphokinase 1 HAMAP MF_00583		PRO_0000141205
Regions
	Region	217 – 230	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	130	1	Magnesium Potential
	Metal binding	132	1	Magnesium Potential
	Metal binding	141	1	Magnesium Potential
	Metal binding	145	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	P65240-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 11EE10055D7F1FE7	FASTA	322	35,451
	10 20 30 40 50 60 MSFSDLKLFA LSSNKELAER VAQEIGIELG KSSVRQFSDG EIQVNIEESI RGKHVFILQS 70 80 90 100 110 120 TSSPVNDNLL EILIMVDALK RASAESVNVV MPYYGYARQD RKARAREPIT SKLVANMLEV 130 140 150 160 170 180 AGVDRLLTID LHAAQIQGFF DIPVDHLMGA PLIADYFERR GMVGSDYVVV SPDHGGVTRA 190 200 210 220 230 240 RKLAEFLKTS IAIIDKRRSV DKMNTSEVMN IIGKVEGKTC ILIDDMIDTA GTICHAADAL 250 260 270 280 290 300 AEAGAVEVYA SCTHPVLSGP ATDNIQKSAI KKLVVLDTIY LPEERLIDKI EQISIAHLLG 310 320 DAIVRIHEKR PLSPLFDIEK KI

References

[1]

"Genome of the bacterium Streptococcus pneumoniae strain R6." Hoskins J., Alborn W.E. Jr., Arnold J., Blaszczak L.C., Burgett S., DeHoff B.S., Estrem S.T., Fritz L., Fu D.-J., Fuller W., Geringer C., Gilmour R., Glass J.S., Khoja H., Kraft A.R., Lagace R.E., LeBlanc D.J., Lee L.N. , Lefkowitz E.J., Lu J., Matsushima P., McAhren S.M., McHenney M., McLeaster K., Mundy C.W., Nicas T.I., Norris F.H., O'Gara M., Peery R.B., Robertson G.T., Rockey P., Sun P.-M., Winkler M.E., Yang Y., Young-Bellido M., Zhao G., Zook C.A., Baltz R.H., Jaskunas S.R., Rosteck P.R. Jr., Skatrud P.L., Glass J.I. J. Bacteriol. 183:5709-5717(2001) [PubMed: 11544234] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE007317 Genomic DNA. Translation: AAK98832.1. Different initiation.
PIR	D97875.
RefSeq	NP_357622.1.
3D structure databases
HSSP	HSSP built from PDB template 1DKR based on UniProtKB P14193.
ModBase	Search...
Protein-protein interaction databases
STRING	P65240.
Genome annotation databases
GeneID	932853.
GenomeReviews	Gene locus spr0028 in contig AE007317_GR.
KEGG	spr:spr0028.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P65240.
Enzyme and pathway databases
BioCyc	SPNE171101:SPR0028-MON.
Family and domain databases
HAMAP	MF_00583. [Tree]
InterPro	IPR000842. PRib-PP_synthetase_CS. IPR000836. PRibTrfase. IPR005946. PRPP_kinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. ribP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHETASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS1_STRR6

Accession

Primary (citable) accession number: P65240
Secondary accession number(s): Q97TB4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	September 22, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)