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Protein

Ribose-phosphate pyrophosphokinase

Gene

prs

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib-5-P).UniRule annotation

Catalytic activityi

ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 Mg2+ ions per subunit.UniRule annotation

Pathwayi: 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Ribose-phosphate pyrophosphokinase (prs)
This subpathway is part of the pathway 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I), the pathway 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi138Magnesium 1UniRule annotation1
Metal bindingi178Magnesium 2UniRule annotation1
Active sitei202UniRule annotation1
Binding sitei204Ribose-5-phosphateUniRule annotation1
Binding sitei230Ribose-5-phosphateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi45 – 47ATPUniRule annotation3
Nucleotide bindingi104 – 105ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processNucleotide biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMBOV233413:G1GT4-1073-MONOMER
UniPathwayiUPA00087; UER00172

Names & Taxonomyi

Protein namesi
Recommended name:
Ribose-phosphate pyrophosphokinaseUniRule annotation (EC:2.7.6.1UniRule annotation)
Short name:
RPPKUniRule annotation
Alternative name(s):
5-phospho-D-ribosyl alpha-1-diphosphateUniRule annotation
Phosphoribosyl diphosphate synthaseUniRule annotation
Phosphoribosyl pyrophosphate synthaseUniRule annotation
Short name:
P-Rib-PP synthaseUniRule annotation
Short name:
PRPP synthaseUniRule annotation
Short name:
PRPPaseUniRule annotation
Gene namesi
Name:prsUniRule annotation
Synonyms:prsA
Ordered Locus Names:BQ2027_MB1045C
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001419 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001411651 – 326Ribose-phosphate pyrophosphokinaseAdd BLAST326

Interactioni

Subunit structurei

Homohexamer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP65233
SMRiP65233
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni234 – 238Ribose-5-phosphate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the ribose-phosphate pyrophosphokinase family. Class I subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000210449
OMAiFGWARQD

Family and domain databases

CDDicd06223 PRTases_typeI, 1 hit
HAMAPiMF_00583_B RibP_PPkinase_B, 1 hit
InterProiView protein in InterPro
IPR000842 PRib_PP_synth_CS
IPR029099 Pribosyltran_N
IPR000836 PRibTrfase_dom
IPR029057 PRTase-like
IPR005946 Rib-P_diPkinase
IPR037515 Rib-P_diPkinase_bac
PfamiView protein in Pfam
PF14572 Pribosyl_synth, 1 hit
PF13793 Pribosyltran_N, 1 hit
SUPFAMiSSF53271 SSF53271, 1 hit
TIGRFAMsiTIGR01251 ribP_PPkin, 1 hit
PROSITEiView protein in PROSITE
PS00114 PRPP_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

P65233-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHDWTDNRK NLMLFAGRAH PELAEQVAKE LDVHVTSQDA REFANGEIFV
60 70 80 90 100
RFHESVRGCD AFVLQSCPAP VNRWLMEQLI MIDALKRGSA KRITAVMPFY
110 120 130 140 150
PYARQDKKHR GREPISARLI ADLLKTAGAD RIVTVDLHTD QIQGFFDGPV
160 170 180 190 200
DHMRGQNLLT GYIRDNYPDG NMVVVSPDSG RVRIAEKWAD ALGGVPLAFI
210 220 230 240 250
HKTRDPRVPN QVVSNRVVGD VAGRTCVLID DMIDTGGTIA GAVALLHNDG
260 270 280 290 300
AGDVIIAATH GVLSDPAAQR LASCGAREVI VTNTLPIGED KRFPQLTVLS
310 320
IAPLLASTIR AVFENGSVTG LFDGDA
Length:326
Mass (Da):35,477
Last modified:October 11, 2004 - v1
Checksum:i4CE64D17E783AC37
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LT708304 Genomic DNA Translation: SIT99644.1
RefSeqiNP_854701.1, NC_002945.3
WP_003405263.1, NC_002945.4

Genome annotation databases

EnsemblBacteriaiCDO42290; CDO42290; Mb1045c
PATRICifig|233413.5.peg.1136

Similar proteinsi

Entry informationi

Entry nameiKPRS_MYCBO
AccessioniPrimary (citable) accession number: P65233
Secondary accession number(s): A0A1R3XX57, P96383, X2BH02
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: April 25, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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