P65219 (KGUA_STAAM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanylate kinase
EC=2.7.4.8

Alternative name(s):
GMP kinase

Gene names

Name:	gmk
Ordered Locus Names:	SAV1209

Organism

Staphylococcus aureus (strain Mu50 / ATCC 700699) [Complete proteome] [HAMAP]

Taxonomic identifier

158878 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Staphylococcus aureus subsp. aureus

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for recycling GMP and indirectly, cGMP By similarity. HAMAP-Rule MF_00328
Catalytic activity	ATP + GMP = ADP + GDP. HAMAP-Rule MF_00328
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the guanylate kinase family. Contains 1 guanylate kinase-like domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	purine nucleotide metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW guanylate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 207	207	Guanylate kinase HAMAP-Rule MF_00328		PRO_0000170604
Regions
	Domain	6 – 185	180	Guanylate kinase-like
	Nucleotide binding	13 – 20	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P65219 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: FE433434A722C98F	FASTA	207	24,022
	10 20 30 40 50 60 MDNEKGLLIV LSGPSGVGKG TVRKRIFEDP STSYKYSISM TTRQMREGEV DGVDYFFKTR 70 80 90 100 110 120 DAFEALIKDD QFIEYAEYVG NYYGTPVQYV KDTMDEGHDV FLEIEVEGAK QVRKKFPDAL 130 140 150 160 170 180 FIFLAPPSLD HLRERLVGRG TESNEKIQSR INEARKEVEM MNLYDYVVVN DEVELAKNRI 190 200 QCIVEAEHLK RERVEAKYRK MILEAKK

References

[1]

"Whole genome sequencing of meticillin-resistant Staphylococcus aureus." Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. , Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H., Hiramatsu K. Lancet 357:1225-1240(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Mu50 / ATCC 700699.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000017 Genomic DNA. Translation: BAB57371.1.
RefSeq	NP_371733.1. NC_002758.2.
3D structure databases
ProteinModelPortal	P65219.
SMR	P65219. Positions 5-197.
ModBase	Search...
Protein-protein interaction databases
STRING	158878.SAV1209.
2D gel databases
World-2DPAGE	0002:P65219.
Proteomic databases
PRIDE	P65219.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB57371; BAB57371; SAV1209.
GeneID	1121186.
KEGG	sav:SAV1209.
PATRIC	19563131. VBIStaAur52173_1243.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0194.
HOGENOM	HOG000037639.
KO	K00942.
OMA	EWAEFAG.
ProtClustDB	PRK00300.
Enzyme and pathway databases
BioCyc	SAUR158878:GJJ5-1227-MONOMER.
Family and domain databases
HAMAP	MF_00328. Guanylate_kinase.
InterPro	IPR008144. Guanylate_kin. IPR008145. Guanylate_kin/L-typ_Ca_channel. IPR020590. Guanylate_kinase_CS. IPR017665. Guanylate_kinase_sub. [Graphical view]
Pfam	PF00625. Guanylate_kin. 1 hit. [Graphical view]
SMART	SM00072. GuKc. 1 hit. [Graphical view]
TIGRFAMs	TIGR03263. guanyl_kin. 1 hit.
PROSITE	PS00856. GUANYLATE_KINASE_1. 1 hit. PS50052. GUANYLATE_KINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KGUA_STAAM

Accession

Primary (citable) accession number: P65219
Secondary accession number(s): Q99UQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program