P65217 (KGUA_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanylate kinase
EC=2.7.4.8

Alternative name(s):
GMP kinase

Gene names

Name:	gmk
Ordered Locus Names:	BMEI1469

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Essential for recycling GMP and indirectly, cGMP By similarity. HAMAP MF_00328
Catalytic activity	ATP + GMP = ADP + GDP. HAMAP MF_00328
Subcellular location	Cytoplasm By similarity HAMAP MF_00328.
Sequence similarities	Belongs to the guanylate kinase family. Contains 1 guanylate kinase-like domain.
Sequence caution	The sequence AAL52650.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW guanylate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 220	220	Guanylate kinase HAMAP MF_00328		PRO_0000170508
Regions
	Domain	14 – 194	181	Guanylate kinase-like
	Nucleotide binding	21 – 28	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P65217 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: C21405890D05491F	FASTA	220	25,223
	10 20 30 40 50 60 MAISSVENGV ARRGLMVVIS SPSGAGKSTI ARLLLEDPKM KLSLSISVTT RQRRPSEIDG 70 80 90 100 110 120 VHYHFITIRE FERLRDNDEL IEWAEVHGNF YGTLRETAEI ALADGQDMLF DIDWQGADQL 130 140 150 160 170 180 QAKMPADVVS IFILPPTMRE LQQRLNRRAE DTAEVIETRL QNARFEIQKW VKYDYIVINE 190 200 210 220 DLDRSYAAIK SIINAERLRR DRRPGLFDFV TGLLEEDPGM

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed: 11756688] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL52650.1. Different initiation.
PIR	AG3435.
RefSeq	NP_540386.2. NC_003317.1.
3D structure databases
ProteinModelPortal	P65217.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1197180.
GenomeReviews	Gene locus BMEI1469 in contig AE008917_GR.
KEGG	bme:BMEI1469.
PATRIC	17853294. VBIBruMel92729_1636.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671982.
OMA	DIDWQGT.
PhylomeDB	P65217.
ProtClustDB	PRK00300.
Enzyme and pathway databases
BioCyc	BMEL224914:BMEI1469-MONOMER.
Family and domain databases
HAMAP	MF_00328. Guanylate_kinase. [Tree]
InterPro	IPR008144. Guanylate_kin. IPR008145. Guanylate_kin/L-typ_Ca_channel. IPR020590. Guanylate_kinase_CS. IPR017665. Guanylate_kinase_sub. [Graphical view]
KO	K00942.
Pfam	PF00625. Guanylate_kin. 1 hit. [Graphical view]
SMART	SM00072. GuKc. 1 hit. [Graphical view]
TIGRFAMs	TIGR03263. Guanyl_kin. 1 hit.
PROSITE	PS00856. GUANYLATE_KINASE_1. 1 hit. PS50052. GUANYLATE_KINASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KGUA_BRUME

Accession

Primary (citable) accession number: P65217
Secondary accession number(s): Q8YFQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program