Reviewed, UniProtKB/Swiss-Prot P65176 (ISPD1_STAAM)

Names and origin

Protein names

Recommended name:
    2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1
    EC=2.7.7.60
Alternative name(s):
    4-diphosphocytidyl-2C-methyl-D-erythritol synthase 1
    MEP cytidylyltransferase 1
      Short name=MCT 1

Gene names

Name:	ispD1
Synonyms:	ispD
Ordered Locus Names:	SAV0251

Organism

Staphylococcus aureus (strain Mu50 / ATCC 700699) [Complete proteome] [HAMAP]

Taxonomic identifier

158878 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity.
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the ispD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 1 HAMAP MF_00108		PRO_0000075615
Sites
	Site	14	1	Transition state stabilizer By similarity
	Site	22	1	Transition state stabilizer By similarity
	Site	160	1	Positions MEP for the nucleophilic attack By similarity
	Site	217	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	P65176-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: A2C4EB712DFC764E	FASTA	238	26,575
	10 20 30 40 50 60 MIYAGILAGG IGSRMGNVPL PKQFLDIDNK PILIHTIEKF ILVSEFNEII IATPAQWISH 70 80 90 100 110 120 TQDILKKYNI TDQRVKVVAG GTDRNETIMN IIDHIRNVNG INNDDVIVTH DAVRPFLTQR 130 140 150 160 170 180 IIKENIEVAA KYGAVDTVIE AIDTIVMSKD KQNIHSIPVR NEMYQGQTPQ SFNIKLLQDS 190 200 210 220 230 YRALSSEQKE ILSDACKIIV ESGHAVKLVR GELYNIKVTT PYDLKVANAI IQGDIADD

References

[1]

"Whole genome sequencing of meticillin-resistant Staphylococcus aureus." Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. , Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H., Hiramatsu K. Lancet 357:1225-1240(2001) [PubMed: 11418146] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000017 Genomic DNA. Translation: BAB56413.1.
RefSeq	NP_370775.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P65176.
2-D gel databases
World-2DPAGE	0002:P65176.
Genome annotation databases
GeneID	1120209.
GenomeReviews	Gene locus SAV0251 in contig BA000017_GR.
KEGG	sav:SAV0251.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P65176.
OMA	GELFNIK.
Enzyme and pathway databases
BioCyc	SAUR158878:SAV0251-MON.
Family and domain databases
HAMAP	MF_00108. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
Pfam	PF01128. IspD. 1 hit. [Graphical view]
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPD1_STAAM

Accession

Primary (citable) accession number: P65176
Secondary accession number(s): Q99WX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	November 3, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)