P65149 (ILVC_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase
EC=1.1.1.86

Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	Rv3001c, MT3081
ORF Names:	MTV012.15c

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.
Sequence caution	The sequence AAK47410.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW growth Inferred from mutant phenotype. Source: MTBBASE
Cellular component	cytosol Inferred from direct assay. Source: MTBBASE plasma membrane Inferred from direct assay. Source: MTBBASE
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_0000151329
Sites
	Active site	105	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	P65149 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 49669E3EDACE25EC	FASTA	333	36,090
	10 20 30 40 50 60 MFYDDDADLS IIQGRKVGVI GYGSQGHAHS LSLRDSGVQV RVGLKQGSRS RPKVEEQGLD 70 80 90 100 110 120 VDTPAEVAKW ADVVMVLAPD TAQAEIFAGD IEPNLKPGDA LFFGHGLNVH FGLIKPPADV 130 140 150 160 170 180 AVAMVAPKGP GHLVRRQFVD GKGVPCLVAV EQDPRGDGLA LALSYAKAIG GTRAGVIKTT 190 200 210 220 230 240 FKDETETDLF GEQTVLCGGT EELVKAGFEV MVEAGYPAEL AYFEVLHELK LIVDLMYEGG 250 260 270 280 290 300 LARMYYSVSD TAEFGGYLSG PRVIDAGTKE RMRDILREIQ DGSFVHKLVA DVEGGNKQLE 310 320 330 ELRRQNAEHP IEVVGKKLRD LMSWVDRPIT ETA

References

[1]

"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.

[2]

"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842581 Genomic DNA. Translation: CAA16086.1. AE000516 Genomic DNA. Translation: AAK47410.1. Different initiation.
PIR	D70855.
RefSeq	NP_337596.1. NC_002755.2.
3D structure databases
ProteinModelPortal	P65149.
SMR	P65149. Positions 1-325.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000003191; EBMYCP00000003191; EBMYCG00000003189. EBMYCT00000069934; EBMYCP00000067993; EBMYCG00000069929.
GeneID	926692.
GenomeReviews	Gene locus MT3081 in contig AE000516_GR. Gene locus Rv3001c in contig AL123456_GR.
KEGG	mtc:MT3081. mtu:Rv3001c.
PATRIC	18128542. VBIMycTub22151_3369.
TIGR	MT3081.
Organism-specific databases
TubercuList	Rv3001c.
Phylogenomic databases
GeneTree	EBGT00050000015910.
HOGENOM	HBG286525.
OMA	EYANGRG.
PhylomeDB	P65149.
ProtClustDB	PRK05479.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. [Graphical view]
Gene3D	G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00053.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_MYCTU

Accession

Primary (citable) accession number: P65149
Secondary accession number(s): O53248

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program