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Protein

Toxin GhoT

Gene

ghoT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Toxic component of a type V toxin-antitoxin (TA) system. Causes membrane damage when induced by MqsR, slowing cell growth and increasing the formation of dormant persister cells; involved with GhoS, its antitoxin, in reducing cell growth during antibacterial stress (PubMed:24373067). Overexpression causes cell lysis, forming ghost cells; both effects are neutralized by expression of GhoS. Overexpression in the presence of ampicillin increases persister cell formation (persister cells exhibit antibiotic tolerance without genetic change) (PubMed:22941047). Overexpression causes about 90-fold reduction in cellular ATP levels and dissipates the membrane potential (PubMed:24373067).2 Publications

GO - Biological processi

  • cell death Source: EcoCyc

Keywordsi

Biological processToxin-antitoxin system

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-2694

Names & Taxonomyi

Protein namesi
Recommended name:
Toxin GhoT1 Publication
Gene namesi
Name:ghoT1 Publication
Synonyms:yjdO
Ordered Locus Names:b4559, JW5732
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14342 ghoT

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei7 – 27HelicalSequence analysisAdd BLAST21
Transmembranei37 – 57HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • cell pole Source: EcoCyc
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: EcoCyc
  • plasma membrane Source: UniProtKB-SubCell

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible effect in the absence of stress (PubMed:24373067). Reduces MqsR-mediated persistence (overexpression of MqsR increases persister cell formation). Decreased biofilm formation after 8 hours at 30 and 37 degrees Celsius, biofilm production has risen by 24 hours. Slight decrease in swimming motility (PubMed:22941047). Cells grown in 20 µg/ml ampicillin in the absence of ghoT and which overexpress MqsR elongate, suggesting MqsR inhibits cell elongation via ghoT (PubMed:23289863). When single mutant is grown in the presence of antibiotics carbenicillin or cefoxitin initial metabolism is significantly increased over that of wild-type, after 14 hours wild-type is slighlty less active. In a double ghoS-ghoT mutant in presence of the 2 antibiotics metabolism is significantly increased over that of wild-type, but by 9 hours wild-type has caught up and eventually has slightly greater metablic rates (PubMed:24373067).3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1M → T: Not toxic in a disrupted ghoS strain as no protein produced. 1 Publication1
Mutagenesisi21I → R: Protein remains toxic. 1 Publication1
Mutagenesisi38F → R: Not toxic, protein still targeted to cell pole. No change in intracellular ATP levels, no dissipation of the membrane potential. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001697351 – 57Toxin GhoTAdd BLAST57

Proteomic databases

PaxDbiP64646
PRIDEiP64646

Expressioni

Inductioni

Expression controlled by its antitoxin GhoS, which digests ghoT transcripts in a sequence-specific manner (PubMed:22941047). Post-transcriptionally regulated by MqsR which acts on the ghoST transcript selectively, degrading the ghoS segment while leaving ghoT intact; conditions which induce MqsR (e.g. overexpression, nalidixic acid, azolocillin or H2O2) decrease ghoS expression and thus increase ghoT transcripts (PubMed:23289863).2 Publications

Interactioni

Protein-protein interaction databases

BioGridi4261768, 10 interactors
STRINGi316385.ECDH10B_4321

Structurei

3D structure databases

ProteinModelPortaliP64646
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GhoT/OrtT toxin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000219287
KOiK18839
OMAiITWYLAR

Family and domain databases

InterProiView protein in InterPro
IPR019689 Toxin_GhoT/OrtT
PfamiView protein in Pfam
PF10753 Toxin_GhoT_OrtT, 1 hit

Sequencei

Sequence statusi: Complete.

P64646-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALFSKILIF YVIGVNISFV IIWFISHEKT HIRLLSAFLV GITWPMSLPV

ALLFSLF
Length:57
Mass (Da):6,555
Last modified:October 11, 2004 - v1
Checksum:iA3670A19500F75D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: ABD18711.1
AP009048 Genomic DNA Translation: BAE78131.1
RefSeqiWP_001173343.1, NZ_LN832404.1
YP_588474.1, NC_000913.3

Genome annotation databases

EnsemblBacteriaiABD18711; ABD18711; b4559
BAE78131; BAE78131; BAE78131
GeneIDi1450308
KEGGiecj:JW5732
eco:b4559
PATRICifig|511145.12.peg.4260

Similar proteinsi

Entry informationi

Entry nameiGHOT_ECOLI
AccessioniPrimary (citable) accession number: P64646
Secondary accession number(s): P58038, Q2EEU1, Q2M6H5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: March 28, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health