Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

GMP/IMP nucleotidase YrfG

Gene

yrfG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the dephosphorylation of different purine nucleotides (GMP and IMP). Also hydrolyzes flavin mononucleotide (FMN).1 Publication

Catalytic activityi

A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.

Cofactori

Mg2+1 Publication, Mn2+1 Publication, Co2+1 Publication, Zn2+1 PublicationNote: Magnesium. Can also use other divalent metal cations as manganese, cobalt or zinc.1 Publication

Kineticsi

  1. KM=0.010 mM for IMP (with manganese ions as cofactor and at pH 9)1 Publication
  2. KM=0.012 mM for GMP (with manganese ions as cofactor and at pH 9)1 Publication
  3. KM=0.062 mM for GMP (with magnesium ions as cofactor and at pH 9)1 Publication
  4. KM=0.22 mM for IMP (with magnesium ions as cofactor and at pH 9)1 Publication

    pH dependencei

    Optimum pH is between 6 and 7.5.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei9NucleophileBy similarity1
    Metal bindingi9MagnesiumBy similarity1
    Metal bindingi11MagnesiumBy similarity1
    Binding sitei149SubstrateBy similarity1
    Metal bindingi174MagnesiumBy similarity1

    GO - Molecular functioni

    • GMP 5'-nucleotidase activity Source: EcoCyc
    • IMP 5'-nucleotidase activity Source: EcoCyc
    • magnesium ion binding Source: UniProtKB
    • manganese ion binding Source: UniProtKB
    • purine nucleosidase activity Source: EcoliWiki

    GO - Biological processi

    • dephosphorylation Source: EcoliWiki

    Keywordsi

    Molecular functionHydrolase
    LigandMagnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:G7742-MONOMER
    MetaCyc:G7742-MONOMER

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GMP/IMP nucleotidase YrfG (EC:3.1.3.5)
    Gene namesi
    Name:yrfG
    Ordered Locus Names:b3399, JW5865
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG12928 yrfG

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001695421 – 222GMP/IMP nucleotidase YrfGAdd BLAST222

    Proteomic databases

    PaxDbiP64636
    PRIDEiP64636

    Interactioni

    Protein-protein interaction databases

    BioGridi4260978, 19 interactors
    STRINGi316385.ECDH10B_3574

    Structurei

    3D structure databases

    ProteinModelPortaliP64636
    SMRiP64636
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni9 – 11Substrate bindingBy similarity3

    Sequence similaritiesi

    Belongs to the HAD-like hydrolase superfamily.Curated

    Phylogenomic databases

    eggNOGiENOG4107Y3D Bacteria
    COG1011 LUCA
    HOGENOMiHOG000271571
    InParanoidiP64636
    KOiK20881
    OMAiNPDSQKP
    PhylomeDBiP64636

    Family and domain databases

    Gene3Di3.40.50.1000, 1 hit
    InterProiView protein in InterPro
    IPR036412 HAD-like_sf
    IPR006439 HAD-SF_hydro_IA
    IPR023214 HAD_sf
    PRINTSiPR00413 HADHALOGNASE
    SUPFAMiSSF56784 SSF56784, 1 hit
    TIGRFAMsiTIGR01509 HAD-SF-IA-v3, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P64636-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MHINIAWQDV DTVLLDMDGT LLDLAFDNYF WQKLVPETWG AKNGVTPQEA
    60 70 80 90 100
    MEYMRQQYHD VQHTLNWYCL DYWSEQLGLD ICAMTTEMGP RAVLREDTIP
    110 120 130 140 150
    FLEALKASGK QRILLTNAHP HNLAVKLEHT GLDAHLDLLL STHTFGYPKE
    160 170 180 190 200
    DQRLWHAVAE ATGLKAERTL FIDDSEAILD AAAQFGIRYC LGVTNPDSGI
    210 220
    AEKQYQRHPS LNDYRRLIPS LM
    Length:222
    Mass (Da):25,399
    Last modified:October 11, 2004 - v1
    Checksum:iAC2AB3BCFE779491
    GO

    Sequence cautioni

    The sequence AAA58196 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U18997 Genomic DNA Translation: AAA58196.1 Different initiation.
    U00096 Genomic DNA Translation: AAC76424.2
    AP009048 Genomic DNA Translation: BAE77892.1
    PIRiB65135
    RefSeqiNP_417858.4, NC_000913.3
    WP_001295168.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC76424; AAC76424; b3399
    BAE77892; BAE77892; BAE77892
    GeneIDi947904
    KEGGiecj:JW5865
    eco:b3399
    PATRICifig|1411691.4.peg.3331

    Similar proteinsi

    Entry informationi

    Entry nameiYRFG_ECOLI
    AccessioniPrimary (citable) accession number: P64636
    Secondary accession number(s): P45801, Q2M764
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: October 11, 2004
    Last modified: March 28, 2018
    This is version 97 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Cookie policy

    We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health