Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cell division protein ZapE

Gene

zapE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Reduces the stability of FtsZ polymers in the presence of ATP. Required for cell division under low-oxygen conditions. Hydrolyzes ATP but not GTP.UniRule annotation1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi78 – 858ATPCurated

GO - Molecular functioni

  • ATPase activity Source: EcoCyc
  • ATP binding Source: UniProtKB-HAMAP

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cell cycle, Cell division

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7680-MONOMER.
ECOL316407:JW3201-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division protein ZapEUniRule annotation
Alternative name(s):
Z ring-associated protein ZapEUniRule annotation
Gene namesi
Name:zapEUniRule annotation
Synonyms:yhcM
Ordered Locus Names:b3232, JW3201
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12819. zapE.

Subcellular locationi

GO - Cellular componenti

  • cell division site Source: EcoCyc
  • cytoplasm Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mutants have no growth defect in aerobic conditions. However, inactivation leads to a stress-dependent elongated phenotype (in anaerobiosis or at temperatures over 37 degrees Celsius).1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi84 – 841K → A: Abolishes ATPase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 375375Cell division protein ZapEPRO_0000169487Add
BLAST

Proteomic databases

PaxDbiP64612.

Interactioni

Subunit structurei

Interacts with FtsZ.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi4261913. 10 interactions.
DIPiDIP-48198N.
IntActiP64612. 1 interaction.
STRINGi511145.b3232.

Structurei

3D structure databases

ProteinModelPortaliP64612.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AFG1 ATPase family. ZapE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D6Z. Bacteria.
COG1485. LUCA.
HOGENOMiHOG000259599.
InParanoidiP64612.
KOiK06916.
OMAiMRTHFHR.
PhylomeDBiP64612.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_01919. ZapE. 1 hit.
InterProiIPR005654. ATPase_AFG1-like.
IPR027417. P-loop_NTPase.
IPR030870. ZapE.
[Graphical view]
PANTHERiPTHR12169. PTHR12169. 1 hit.
PfamiPF03969. AFG1_ATPase. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P64612-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSVTPTSQY LKALNEGSHQ PDDVQKEAVS RLEIIYQELI NSTPPAPRTS
60 70 80 90 100
GLMARVGKLW GKREDTKHTP VRGLYMWGGV GRGKTWLMDL FYQSLPGERK
110 120 130 140 150
QRLHFHRFML RVHEELTALQ GQTDPLEIIA DRFKAETDVL CFDEFFVSDI
160 170 180 190 200
TDAMLLGGLM KALFARGITL VATSNIPPDE LYRNGLQRAR FLPAIDAIKQ
210 220 230 240 250
HCDVMNVDAG VDYRLRTLTQ AHLWLSPLHD ETRAQMDKLW LALAGGKREN
260 270 280 290 300
SPTLEINHRP LATMGVENQT LAVSFTTLCV DARSQHDYIA LSRLFHTVML
310 320 330 340 350
FDVPVMTRLM ESEARRFIAL VDEFYERHVK LVVSAEVPLY EIYQGDRLKF
360 370
EFQRCLSRLQ EMQSEEYLKR EHLAG
Length:375
Mass (Da):43,064
Last modified:October 11, 2004 - v1
Checksum:i24866D51E551452D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58034.1.
U00096 Genomic DNA. Translation: AAC76264.1.
AP009048 Genomic DNA. Translation: BAE77275.1.
PIRiB65115.
RefSeqiNP_417699.1. NC_000913.3.
WP_001192332.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76264; AAC76264; b3232.
BAE77275; BAE77275; BAE77275.
GeneIDi947821.
KEGGiecj:JW3201.
eco:b3232.
PATRICi32121890. VBIEscCol129921_3329.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58034.1.
U00096 Genomic DNA. Translation: AAC76264.1.
AP009048 Genomic DNA. Translation: BAE77275.1.
PIRiB65115.
RefSeqiNP_417699.1. NC_000913.3.
WP_001192332.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP64612.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261913. 10 interactions.
DIPiDIP-48198N.
IntActiP64612. 1 interaction.
STRINGi511145.b3232.

Proteomic databases

PaxDbiP64612.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76264; AAC76264; b3232.
BAE77275; BAE77275; BAE77275.
GeneIDi947821.
KEGGiecj:JW3201.
eco:b3232.
PATRICi32121890. VBIEscCol129921_3329.

Organism-specific databases

EchoBASEiEB2670.
EcoGeneiEG12819. zapE.

Phylogenomic databases

eggNOGiENOG4105D6Z. Bacteria.
COG1485. LUCA.
HOGENOMiHOG000259599.
InParanoidiP64612.
KOiK06916.
OMAiMRTHFHR.
PhylomeDBiP64612.

Enzyme and pathway databases

BioCyciEcoCyc:G7680-MONOMER.
ECOL316407:JW3201-MONOMER.

Miscellaneous databases

PROiP64612.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_01919. ZapE. 1 hit.
InterProiIPR005654. ATPase_AFG1-like.
IPR027417. P-loop_NTPase.
IPR030870. ZapE.
[Graphical view]
PANTHERiPTHR12169. PTHR12169. 1 hit.
PfamiPF03969. AFG1_ATPase. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiZAPE_ECOLI
AccessioniPrimary (citable) accession number: P64612
Secondary accession number(s): P46442, Q2M8Y1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: September 7, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

ZapE abundance has to be tightly regulated to allow cell division. Loss or overexpression of ZapE alters Z-ring stability and leads to bacterial filamentation (PubMed:24595368).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.