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Protein

Probable phospholipid ABC transporter permease protein MlaE

Gene

mlaE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex MlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane.1 Publication

GO - Molecular functioni

  • phospholipid binding Source: EcoCyc
  • phospholipid transporter activity Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:YRBE-MONOMER.
ECOL316407:JW3161-MONOMER.

Protein family/group databases

TCDBi3.A.1.27.3. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable phospholipid ABC transporter permease protein MlaECurated
Gene namesi
Name:mlaE
Synonyms:yrbE
Ordered Locus Names:b3194, JW3161
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12800. mlaE.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 50Cytoplasmic1 PublicationAdd BLAST50
Transmembranei51 – 71HelicalSequence analysisAdd BLAST21
Topological domaini72 – 88Periplasmic1 PublicationAdd BLAST17
Transmembranei89 – 109HelicalSequence analysisAdd BLAST21
Topological domaini110 – 147Cytoplasmic1 PublicationAdd BLAST38
Transmembranei148 – 168HelicalSequence analysisAdd BLAST21
Topological domaini169 – 198Periplasmic1 PublicationAdd BLAST30
Transmembranei199 – 219HelicalSequence analysisAdd BLAST21
Topological domaini220 – 238Cytoplasmic1 PublicationAdd BLAST19
Transmembranei239 – 259HelicalSequence analysisAdd BLAST21
Topological domaini260Periplasmic1 Publication1

GO - Cellular componenti

  • ATP-binding cassette (ABC) transporter complex Source: InterPro
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutation confers sensitivity to SDS-EDTA and leads to accumulation of phospholipid in the outer leaflet of the outer membrane.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001694651 – 260Probable phospholipid ABC transporter permease protein MlaEAdd BLAST260

Proteomic databases

PaxDbiP64606.
PRIDEiP64606.

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (MlaF), two transmembrane proteins (MlaE), two cytoplasmic solute-binding proteins (MlaB) and a periplamic solute-binding protein (MlaD).1 Publication

Protein-protein interaction databases

BioGridi4263406. 8 interactors.
STRINGi511145.b3194.

Structurei

3D structure databases

ProteinModelPortaliP64606.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MlaE permease family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CY3. Bacteria.
COG0767. LUCA.
HOGENOMiHOG000255130.
InParanoidiP64606.
KOiK02066.
OMAiQLMESGP.
PhylomeDBiP64606.

Family and domain databases

InterProiIPR003453. ABC_MlaE_Proteobac.
IPR030802. Permease_MalE.
[Graphical view]
PANTHERiPTHR30188. PTHR30188. 1 hit.
PfamiPF02405. MlaE. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00056. TIGR00056. 1 hit.

Sequencei

Sequence statusi: Complete.

P64606-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLNALASLG HKGIKTLRTF GRAGLMLFNA LVGKPEFRKH APLLVRQLYN
60 70 80 90 100
VGVLSMLIIV VSGVFIGMVL GLQGYLVLTT YSAETSLGML VALSLLRELG
110 120 130 140 150
PVVAALLFAG RAGSALTAEI GLMRATEQLS SMEMMAVDPL RRVISPRFWA
160 170 180 190 200
GVISLPLLTV IFVAVGIWGG SLVGVSWKGI DSGFFWSAMQ NAVDWRMDLV
210 220 230 240 250
NCLIKSVVFA ITVTWISLFN GYDAIPTSAG ISRATTRTVV HSSLAVLGLD
260
FVLTALMFGN
Length:260
Mass (Da):27,863
Last modified:October 11, 2004 - v1
Checksum:i057409EA1E6E25EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57995.1.
U00096 Genomic DNA. Translation: AAC76226.1.
AP009048 Genomic DNA. Translation: BAE77238.1.
PIRiD65110.
RefSeqiNP_417661.1. NC_000913.3.
WP_000925795.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76226; AAC76226; b3194.
BAE77238; BAE77238; BAE77238.
GeneIDi947732.
KEGGiecj:JW3161.
eco:b3194.
PATRICi32121808. VBIEscCol129921_3288.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57995.1.
U00096 Genomic DNA. Translation: AAC76226.1.
AP009048 Genomic DNA. Translation: BAE77238.1.
PIRiD65110.
RefSeqiNP_417661.1. NC_000913.3.
WP_000925795.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP64606.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263406. 8 interactors.
STRINGi511145.b3194.

Protein family/group databases

TCDBi3.A.1.27.3. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP64606.
PRIDEiP64606.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76226; AAC76226; b3194.
BAE77238; BAE77238; BAE77238.
GeneIDi947732.
KEGGiecj:JW3161.
eco:b3194.
PATRICi32121808. VBIEscCol129921_3288.

Organism-specific databases

EchoBASEiEB2652.
EcoGeneiEG12800. mlaE.

Phylogenomic databases

eggNOGiENOG4105CY3. Bacteria.
COG0767. LUCA.
HOGENOMiHOG000255130.
InParanoidiP64606.
KOiK02066.
OMAiQLMESGP.
PhylomeDBiP64606.

Enzyme and pathway databases

BioCyciEcoCyc:YRBE-MONOMER.
ECOL316407:JW3161-MONOMER.

Miscellaneous databases

PROiP64606.

Family and domain databases

InterProiIPR003453. ABC_MlaE_Proteobac.
IPR030802. Permease_MalE.
[Graphical view]
PANTHERiPTHR30188. PTHR30188. 1 hit.
PfamiPF02405. MlaE. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00056. TIGR00056. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMLAE_ECOLI
AccessioniPrimary (citable) accession number: P64606
Secondary accession number(s): P45392, Q2M918
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: November 2, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.