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Protein

Probable phospholipid ABC transporter-binding protein MlaD

Gene

mlaD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex MlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane.1 Publication

GO - Molecular functioni

  • phospholipid binding Source: EcoCyc
  • phospholipid transporter activity Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG12799-MONOMER.
ECOL316407:JW3160-MONOMER.

Protein family/group databases

TCDBi3.A.1.27.3. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable phospholipid ABC transporter-binding protein MlaDCurated
Gene namesi
Name:mlaD
Synonyms:yrbD
Ordered Locus Names:b3193, JW3160
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12799. mlaD.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77Cytoplasmic1 Publication
Transmembranei8 – 2821Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini29 – 183155Periplasmic1 PublicationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutation confers sensitivity to SDS-EDTA and leads to accumulation of phospholipid in the outer leaflet of the outer membrane.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 183183Probable phospholipid ABC transporter-binding protein MlaDPRO_0000013918Add
BLAST

Proteomic databases

EPDiP64604.
PaxDbiP64604.
PRIDEiP64604.

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (MlaF), two transmembrane proteins (MlaE), two cytoplasmic solute-binding proteins (MlaB) and a periplamic solute-binding protein (MlaD).1 Publication

Protein-protein interaction databases

BioGridi4262424. 6 interactions.
DIPiDIP-12906N.
IntActiP64604. 1 interaction.
MINTiMINT-6478352.
STRINGi511145.b3193.

Structurei

3D structure databases

ProteinModelPortaliP64604.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MlaD family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4107PE3. Bacteria.
COG1463. LUCA.
HOGENOMiHOG000255143.
InParanoidiP64604.
KOiK02067.
OMAiEQYIGLE.
OrthoDBiEOG6F296G.
PhylomeDBiP64604.

Family and domain databases

InterProiIPR030970. ABC_MlaD.
IPR003399. Mce/MlaD.
[Graphical view]
PfamiPF02470. MlaD. 1 hit.
[Graphical view]
TIGRFAMsiTIGR04430. OM_asym_MlaD. 1 hit.

Sequencei

Sequence statusi: Complete.

P64604-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQTKKNEIWV GIFLLAALLA ALFVCLKAAN VTSIRTEPTY TLYATFDNIG
60 70 80 90 100
GLKARSPVSI GGVVVGRVAD ITLDPKTYLP RVTLEIEQRY NHIPDTSSLS
110 120 130 140 150
IRTSGLLGEQ YLALNVGFED PELGTAILKD GDTIQDTKSA MVLEDLIGQF
160 170 180
LYGSKGDDNK NSGDAPAAAP GNNETTEPVG TTK
Length:183
Mass (Da):19,576
Last modified:October 11, 2004 - v1
Checksum:i6BD8B922002EFD28
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57994.1.
U00096 Genomic DNA. Translation: AAC76225.1.
AP009048 Genomic DNA. Translation: BAE77237.1.
PIRiC65110.
RefSeqiNP_417660.1. NC_000913.3.
WP_001296448.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76225; AAC76225; b3193.
BAE77237; BAE77237; BAE77237.
GeneIDi947712.
KEGGiecj:JW3160.
eco:b3193.
PATRICi32121806. VBIEscCol129921_3287.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57994.1.
U00096 Genomic DNA. Translation: AAC76225.1.
AP009048 Genomic DNA. Translation: BAE77237.1.
PIRiC65110.
RefSeqiNP_417660.1. NC_000913.3.
WP_001296448.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP64604.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262424. 6 interactions.
DIPiDIP-12906N.
IntActiP64604. 1 interaction.
MINTiMINT-6478352.
STRINGi511145.b3193.

Protein family/group databases

TCDBi3.A.1.27.3. the atp-binding cassette (abc) superfamily.

Proteomic databases

EPDiP64604.
PaxDbiP64604.
PRIDEiP64604.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76225; AAC76225; b3193.
BAE77237; BAE77237; BAE77237.
GeneIDi947712.
KEGGiecj:JW3160.
eco:b3193.
PATRICi32121806. VBIEscCol129921_3287.

Organism-specific databases

EchoBASEiEB2651.
EcoGeneiEG12799. mlaD.

Phylogenomic databases

eggNOGiENOG4107PE3. Bacteria.
COG1463. LUCA.
HOGENOMiHOG000255143.
InParanoidiP64604.
KOiK02067.
OMAiEQYIGLE.
OrthoDBiEOG6F296G.
PhylomeDBiP64604.

Enzyme and pathway databases

BioCyciEcoCyc:EG12799-MONOMER.
ECOL316407:JW3160-MONOMER.

Miscellaneous databases

PROiP64604.

Family and domain databases

InterProiIPR030970. ABC_MlaD.
IPR003399. Mce/MlaD.
[Graphical view]
PfamiPF02470. MlaD. 1 hit.
[Graphical view]
TIGRFAMsiTIGR04430. OM_asym_MlaD. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "An ABC transport system that maintains lipid asymmetry in the gram-negative outer membrane."
    Malinverni J.C., Silhavy T.J.
    Proc. Natl. Acad. Sci. U.S.A. 106:8009-8014(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN PHOSPHOLIPID TRANSPORT, SUBUNIT, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
    Strain: K12 / MC4100 / JA176.

Entry informationi

Entry nameiMLAD_ECOLI
AccessioniPrimary (citable) accession number: P64604
Secondary accession number(s): P45391, Q2M919
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: April 13, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.