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Protein

Ribonuclease toxin YhaV

Gene

yhaV

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Toxic component of a type II toxin-antitoxin (TA) system. Has RNase activity in vitro. Overexpression leads to growth arrest after 30 minutes; these effects are overcome by concomitant expression of antitoxin SohA (PrlF). Massive overexpression is toxic. Unlike most other characterized TA systems degrades rRNA, and co-folding of the both TA proteins is necessary in vitro for inhibition of the RNase activity. It is not known if it has any sequence-specificity. Acts as a transcription factor. The YhaV/PrlF complex binds the prlF-yhaV operon, probably negatively regulating its expression. mRNA interferases play a role in bacterial persistence to antibiotics (PubMed:21788497).2 Publications

GO - Molecular functioni

  • endonuclease activity Source: UniProtKB-KW
  • ribonuclease activity Source: EcoCyc

GO - Biological processi

  • negative regulation of cell growth Source: EcoCyc
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • RNA phosphodiester bond hydrolysis Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease, Repressor
Biological processToxin-antitoxin system, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:G7629-MONOMER
MetaCyc:G7629-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease toxin YhaV (EC:3.1.-.-)
Alternative name(s):
Ribonuclease YhaV
Gene namesi
Name:yhaV
Ordered Locus Names:b3130, JW3099
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12761 yhaV

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Disruption phenotypei

mRNA interferases play a role in bacterial persistence to antibiotics; as 10 mRNA interferases are successively deleted reduced levels of persisters are generated (PubMed:21788497).1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi85 – 94RVKFGAGRYR → AVKFGAGRYA: Complete loss of toxicity. 1 Publication10
Mutagenesisi85R → A: Partial loss of toxicity. 1 Publication1
Mutagenesisi94R → A: Partial loss of toxicity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001694501 – 154Ribonuclease toxin YhaVAdd BLAST154

Proteomic databases

PaxDbiP64594
PRIDEiP64594

Interactioni

Subunit structurei

Homohexamer; forms a complex with PrlF (SohA) with stoichiometry PrlF2-YhaV4, possibly as a YhaV2-PrlF2-YhaV2 complex like the MazFE complex. This complex is seen to dimerize in solution.1 Publication

Protein-protein interaction databases

BioGridi4262413, 9 interactors
STRINGi316385.ECDH10B_3303

Structurei

3D structure databases

ProteinModelPortaliP64594
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4108R8Z Bacteria
ENOG4111FPC LUCA
HOGENOMiHOG000229556
InParanoidiP64594
KOiK19155
OMAiMQRHGWT
PhylomeDBiP64594

Family and domain databases

InterProiView protein in InterPro
IPR021679 Toxin_endonuclease_YhaV
PfamiView protein in Pfam
PF11663 Toxin_YhaV, 1 hit

Sequencei

Sequence statusi: Complete.

P64594-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFPQRVNGW ALYAHPCFQE TYDALVAEVE TLKGKDPENY QRKAATKLLA
60 70 80 90 100
VVHKVIEEHI TVNPSSPAFR HGKSLGSGKN KDWSRVKFGA GRYRLFFRYS
110 120 130 140 150
EKEKVIILGW MNDENTLRTY GKKTDAYTVF SKMLKRGHPP ADWETLTRET

EETH
Length:154
Mass (Da):17,836
Last modified:October 11, 2004 - v1
Checksum:i758EBC8263326962
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA Translation: AAA57933.1
U00096 Genomic DNA Translation: AAC76164.1
AP009048 Genomic DNA Translation: BAE77177.1
PIRiF65102
RefSeqiNP_417599.1, NC_000913.3
WP_000347273.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76164; AAC76164; b3130
BAE77177; BAE77177; BAE77177
GeneIDi947638
KEGGiecj:JW3099
eco:b3130
PATRICifig|1411691.4.peg.3601

Similar proteinsi

Entry informationi

Entry nameiYHAV_ECOLI
AccessioniPrimary (citable) accession number: P64594
Secondary accession number(s): P42901, Q2M979
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: March 28, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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