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Protein

Toxin YhaV

Gene

yhaV

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Toxic component of a toxin-antitoxin (TA) module. Has RNase activity in vitro. Overexpression leads to growth arrest after 30 minutes; these effects are overcome by concomitant expression of antitoxin SohA (PrlF). Massive overexpression is toxic. Unlike most other characterized TA systems degrades rRNA, and co-folding of the proteins is necessary in vitro for inhibition of the RNase activity. It is not known if it has any sequence-specificity. Acts as a transcription factor. The YhaV/PrlF complex binds the prlF-yhaV operon, probably negatively regulating its expression.1 Publication

GO - Molecular functioni

  • endonuclease activity Source: UniProtKB-KW
  • ribonuclease activity Source: EcoCyc

GO - Biological processi

  • negative regulation of cell growth Source: EcoCyc
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • RNA phosphodiester bond hydrolysis Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease, Repressor, Toxin

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:G7629-MONOMER.
ECOL316407:JW3099-MONOMER.
MetaCyc:G7629-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Toxin YhaV (EC:3.1.-.-)
Alternative name(s):
Ribonuclease YhaV
Gene namesi
Name:yhaV
Ordered Locus Names:b3130, JW3099
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12761. yhaV.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi85 – 9410RVKFGAGRYR → AVKFGAGRYA: Complete loss of toxicity. 1 Publication
Mutagenesisi85 – 851R → A: Partial loss of toxicity. 1 Publication
Mutagenesisi94 – 941R → A: Partial loss of toxicity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 154154Toxin YhaVPRO_0000169450Add
BLAST

Proteomic databases

PaxDbiP64594.

Interactioni

Subunit structurei

Homohexamer; forms a complex with PrlF (SohA) with stoichiometry PrlF(2)-YhaV4, possibly as a YhaV(2)-PrlF(2)-YhaV2 complex like the MazFE complex. This complex is seen to dimerize in solution.1 Publication

Protein-protein interaction databases

BioGridi4262413. 9 interactions.
STRINGi511145.b3130.

Structurei

3D structure databases

ProteinModelPortaliP64594.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4108R8Z. Bacteria.
ENOG4111FPC. LUCA.
HOGENOMiHOG000229556.
InParanoidiP64594.
KOiK19155.
OMAiAWVNDET.
OrthoDBiEOG6358DR.
PhylomeDBiP64594.

Family and domain databases

InterProiIPR021679. Toxin_endonuclease_YhaV.
[Graphical view]
PfamiPF11663. Toxin_YhaV. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P64594-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFPQRVNGW ALYAHPCFQE TYDALVAEVE TLKGKDPENY QRKAATKLLA
60 70 80 90 100
VVHKVIEEHI TVNPSSPAFR HGKSLGSGKN KDWSRVKFGA GRYRLFFRYS
110 120 130 140 150
EKEKVIILGW MNDENTLRTY GKKTDAYTVF SKMLKRGHPP ADWETLTRET

EETH
Length:154
Mass (Da):17,836
Last modified:October 11, 2004 - v1
Checksum:i758EBC8263326962
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57933.1.
U00096 Genomic DNA. Translation: AAC76164.1.
AP009048 Genomic DNA. Translation: BAE77177.1.
PIRiF65102.
RefSeqiNP_417599.1. NC_000913.3.
WP_000347273.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76164; AAC76164; b3130.
BAE77177; BAE77177; BAE77177.
GeneIDi947638.
KEGGiecj:JW3099.
eco:b3130.
PATRICi32121676. VBIEscCol129921_3224.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57933.1.
U00096 Genomic DNA. Translation: AAC76164.1.
AP009048 Genomic DNA. Translation: BAE77177.1.
PIRiF65102.
RefSeqiNP_417599.1. NC_000913.3.
WP_000347273.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP64594.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262413. 9 interactions.
STRINGi511145.b3130.

Proteomic databases

PaxDbiP64594.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76164; AAC76164; b3130.
BAE77177; BAE77177; BAE77177.
GeneIDi947638.
KEGGiecj:JW3099.
eco:b3130.
PATRICi32121676. VBIEscCol129921_3224.

Organism-specific databases

EchoBASEiEB2614.
EcoGeneiEG12761. yhaV.

Phylogenomic databases

eggNOGiENOG4108R8Z. Bacteria.
ENOG4111FPC. LUCA.
HOGENOMiHOG000229556.
InParanoidiP64594.
KOiK19155.
OMAiAWVNDET.
OrthoDBiEOG6358DR.
PhylomeDBiP64594.

Enzyme and pathway databases

BioCyciEcoCyc:G7629-MONOMER.
ECOL316407:JW3099-MONOMER.
MetaCyc:G7629-MONOMER.

Miscellaneous databases

PROiP64594.

Family and domain databases

InterProiIPR021679. Toxin_endonuclease_YhaV.
[Graphical view]
PfamiPF11663. Toxin_YhaV. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "prlF and yhaV encode a new toxin-antitoxin system in Escherichia coli."
    Schmidt O., Schuenemann V.J., Hand N.J., Silhavy T.J., Martin J., Lupas A.N., Djuranovic S.
    J. Mol. Biol. 372:894-905(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A TOXIN, FUNCTION AS A RIBONUCLEASE, SUBUNIT, MUTAGENESIS OF ARG-85; 85-ARG--ARG-94 AND ARG-94, OPERON STRUCTURE.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "mRNA interferases, sequence-specific endoribonucleases from the toxin-antitoxin systems."
    Yamaguchi Y., Inouye M.
    Prog. Mol. Biol. Transl. Sci. 85:467-500(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiYHAV_ECOLI
AccessioniPrimary (citable) accession number: P64594
Secondary accession number(s): P42901, Q2M979
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: January 20, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.