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Protein

mRNA interferase toxin HigB

Gene

higB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Toxic component of a type II toxin-antitoxin (TA) module. A probable translation-dependent mRNA interferase. Overexpression causes cessation of cell growth and inhibits cell proliferation via inhibition of translation; this blockage is overcome by subsequent expression of antitoxin HigA. Overexpression causes cleavage of a number of mRNAs in a translation-dependent fashion, suggesting this is an mRNA interferase. mRNA interferases play a role in bacterial persistence to antibiotics; overexpression of this protein induces persisters resistant to ciprofloxacin and ampicillin (PubMed:21788497).2 Publications

GO - Molecular functioni

  • endoribonuclease activity Source: EcoCyc
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • negative regulation of translation Source: EcoCyc
  • regulation of mRNA stability Source: EcoCyc

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease, RNA-binding, Toxin
Biological processStress response

Enzyme and pathway databases

BioCyciEcoCyc:G7602-MONOMER.
MetaCyc:G7602-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
mRNA interferase toxin HigB (EC:3.1.-.-)
Alternative name(s):
Endoribonuclease HigB
Toxin HigB
Gene namesi
Name:higB
Synonyms:ygjN
Ordered Locus Names:b3083, JW3054
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12725. higB.

Pathology & Biotechi

Disruption phenotypei

mRNA interferases play a role in bacterial persistence to antibiotics; as 10 mRNA interferases are successively deleted reduced levels of persisters are generated (PubMed:21788497).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001694211 – 104mRNA interferase toxin HigBAdd BLAST104

Proteomic databases

PaxDbiP64578.
PRIDEiP64578.

Expressioni

Inductioni

Induced by amino acid starvation and when translation is blocked. Induction is decreased in the absence of the Lon protease suggesting, by homology to other toxin-antitoxin systems, that Lon may degrade the HigA antitoxin. Transcription is negatively regulated by the cognate locus, probably by HigA. A member of the higB-higA operon.1 Publication

Interactioni

Subunit structurei

Probably forms a complex with the antitoxin HigA which inhibits the mRNA interferase activity.Curated

Protein-protein interaction databases

BioGridi4262402. 8 interactors.
IntActiP64578. 2 interactors.
STRINGi316385.ECDH10B_3258.

Structurei

Secondary structure

1104
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 4Combined sources3
Helixi7 – 15Combined sources9
Helixi17 – 19Combined sources3
Helixi20 – 32Combined sources13
Helixi38 – 44Combined sources7
Beta strandi52 – 54Combined sources3
Beta strandi57 – 62Combined sources6
Turni63 – 66Combined sources4
Beta strandi67 – 74Combined sources8
Turni75 – 78Combined sources4
Beta strandi79 – 87Combined sources9
Helixi88 – 97Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5IFGX-ray2.70A/C1-104[»]
ProteinModelPortaliP64578.
SMRiP64578.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HigB mRNA interferase family.Curated

Phylogenomic databases

eggNOGiENOG4105R48. Bacteria.
COG4680. LUCA.
HOGENOMiHOG000026126.
InParanoidiP64578.
KOiK19166.

Family and domain databases

InterProiView protein in InterPro
IPR018669. Toxin_HigB_put.
PfamiView protein in Pfam
PF09907. HigB_toxin. 1 hit.

Sequencei

Sequence statusi: Complete.

P64578-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLITQKALK DAAEKYPQHK TELVALGNTI AKGYFKKPES LKAVFPSLDN
60 70 80 90 100
FKYLDKHYVF NVGGNELRVV AMVFFESQKC YIREVMTHKE YDFFTAVHRT

KGKK
Length:104
Mass (Da):12,103
Last modified:October 11, 2004 - v1
Checksum:iF23FB5F433C52C22
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57884.1.
U00096 Genomic DNA. Translation: AAC76118.1.
AP009048 Genomic DNA. Translation: BAE77133.1.
PIRiH65096.
RefSeqiNP_417554.1. NC_000913.3.
WP_000550189.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76118; AAC76118; b3083.
BAE77133; BAE77133; BAE77133.
GeneIDi947591.
KEGGiecj:JW3054.
eco:b3083.
PATRICifig|1411691.4.peg.3646.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiHIGB_ECOLI
AccessioniPrimary (citable) accession number: P64578
Secondary accession number(s): P42595, Q2M9C3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: July 5, 2017
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families