P64578 (HIGB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
mRNA interferase HigB
EC=3.1.-.-

Alternative name(s):
Endoribonuclease HigB
Toxin HigB

Gene names

Name:	higB
Synonyms:	ygjN
Ordered Locus Names:	b3083, JW3054

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	104 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Toxic component of a toxin-antitoxin (TA) module. A probable translation-dependent mRNA interferase. Overexpression causes cessation of cell growth and inhibits cell proliferation via inhibition of translation; this blockage is overcome by subsequent expression of antitoxin HigA. Overexpression causes cleavage of a number of mRNAs in a translation-dependent fashion, suggesting this is an mRNA interferase. Ref.3
Subunit structure	Probably forms a complex with the antitoxin HigA which inhibits the mRNA interferase activity By similarity.
Induction	Induced by amino acid starvation and when translation is blocked. Induction is decreased in the absence of the Lon protease suggesting, by homology to other toxin-antitoxin systems, that Lon may degrade the HigA antitoxin. Transcription is negatively regulated by the cognate locus, probably by HigA. A member of the higB-higA operon. Ref.3
Sequence similarities	Belongs to the HigB mRNA interferase family.

Ontologies

Keywords
Biological process	Stress response
Ligand	RNA-binding
Molecular function	Endonuclease Hydrolase Nuclease Toxin
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of translation Inferred from direct assay Ref.3. Source: EcoCyc regulation of mRNA stability Inferred from direct assay Ref.3. Source: EcoCyc response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW endoribonuclease activity Inferred from direct assay Ref.3. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 104	104	mRNA interferase HigB		PRO_0000169421

Sequences

	Sequence		Length	Mass (Da)
	P64578 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: F23FB5F433C52C22	FASTA	104	12,103
	10 20 30 40 50 60 MHLITQKALK DAAEKYPQHK TELVALGNTI AKGYFKKPES LKAVFPSLDN FKYLDKHYVF 70 80 90 100 NVGGNELRVV AMVFFESQKC YIREVMTHKE YDFFTAVHRT KGKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Three new RelE-homologous mRNA interferases of Escherichia coli differentially induced by environmental stresses." Christensen-Dalsgaard M., Jorgensen M.G., Gerdes K. Mol. Microbiol. 75:333-348(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION AS AN MRNA INTERFERASE, INDUCTION, OPERON STRUCTURE. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18997 Genomic DNA. Translation: AAA57884.1. U00096 Genomic DNA. Translation: AAC76118.1. AP009048 Genomic DNA. Translation: BAE77133.1.
PIR	H65096.
RefSeq	NP_417554.1. NC_000913.2. YP_491274.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P64578.
ModBase	Search...
Protein-protein interaction databases
IntAct	P64578. 1 interaction.
STRING	511145.b3083.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76118; AAC76118; b3083. BAE77133; BAE77133; BAE77133.
GeneID	12933408. 947591.
KEGG	ecj:Y75_p3008. eco:b3083.
PATRIC	32121584. VBIEscCol129921_3178.
Organism-specific databases
EchoBASE	EB2584.
EcoGene	EG12725. higB.
Phylogenomic databases
eggNOG	COG4680.
HOGENOM	HOG000026126.
OMA	LTHDEYD.
ProtClustDB	CLSK880573.
Enzyme and pathway databases
BioCyc	EcoCyc:G7602-MONOMER. ECOL316407:JW3054-MONOMER. MetaCyc:G7602-MONOMER.
Gene expression databases
Genevestigator	P64578.
Family and domain databases
InterPro	IPR018669. Toxin_HigB_put. [Graphical view]
Pfam	PF09907. DUF2136. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HIGB_ECOLI

Accession

Primary (citable) accession number: P64578
Secondary accession number(s): P42595, Q2M9C3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program