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Protein

mRNA interferase HigB

Gene

higB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Toxic component of a toxin-antitoxin (TA) module. A probable translation-dependent mRNA interferase. Overexpression causes cessation of cell growth and inhibits cell proliferation via inhibition of translation; this blockage is overcome by subsequent expression of antitoxin HigA. Overexpression causes cleavage of a number of mRNAs in a translation-dependent fashion, suggesting this is an mRNA interferase.1 Publication

GO - Molecular functioni

  • endoribonuclease activity Source: EcoCyc
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • negative regulation of translation Source: EcoCyc
  • regulation of mRNA stability Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease, Toxin

Keywords - Biological processi

Stress response

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7602-MONOMER.
ECOL316407:JW3054-MONOMER.
MetaCyc:G7602-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
mRNA interferase HigB (EC:3.1.-.-)
Alternative name(s):
Endoribonuclease HigB
Toxin HigB
Gene namesi
Name:higB
Synonyms:ygjN
Ordered Locus Names:b3083, JW3054
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12725. higB.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 104104mRNA interferase HigBPRO_0000169421Add
BLAST

Proteomic databases

PaxDbiP64578.

Expressioni

Inductioni

Induced by amino acid starvation and when translation is blocked. Induction is decreased in the absence of the Lon protease suggesting, by homology to other toxin-antitoxin systems, that Lon may degrade the HigA antitoxin. Transcription is negatively regulated by the cognate locus, probably by HigA. A member of the higB-higA operon.1 Publication

Interactioni

Subunit structurei

Probably forms a complex with the antitoxin HigA which inhibits the mRNA interferase activity.Curated

Protein-protein interaction databases

BioGridi4262402. 8 interactions.
IntActiP64578. 2 interactions.
STRINGi511145.b3083.

Structurei

3D structure databases

ProteinModelPortaliP64578.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HigB mRNA interferase family.Curated

Phylogenomic databases

eggNOGiENOG4105R48. Bacteria.
COG4680. LUCA.
HOGENOMiHOG000026126.
InParanoidiP64578.
KOiK19166.
OMAiTIFPSAD.
OrthoDBiEOG6G20P2.

Family and domain databases

InterProiIPR018669. Toxin_HigB_put.
[Graphical view]
PfamiPF09907. HigB_toxin. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P64578-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLITQKALK DAAEKYPQHK TELVALGNTI AKGYFKKPES LKAVFPSLDN
60 70 80 90 100
FKYLDKHYVF NVGGNELRVV AMVFFESQKC YIREVMTHKE YDFFTAVHRT

KGKK
Length:104
Mass (Da):12,103
Last modified:October 11, 2004 - v1
Checksum:iF23FB5F433C52C22
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57884.1.
U00096 Genomic DNA. Translation: AAC76118.1.
AP009048 Genomic DNA. Translation: BAE77133.1.
PIRiH65096.
RefSeqiNP_417554.1. NC_000913.3.
WP_000550189.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76118; AAC76118; b3083.
BAE77133; BAE77133; BAE77133.
GeneIDi947591.
KEGGiecj:JW3054.
eco:b3083.
PATRICi32121584. VBIEscCol129921_3178.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA57884.1.
U00096 Genomic DNA. Translation: AAC76118.1.
AP009048 Genomic DNA. Translation: BAE77133.1.
PIRiH65096.
RefSeqiNP_417554.1. NC_000913.3.
WP_000550189.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP64578.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262402. 8 interactions.
IntActiP64578. 2 interactions.
STRINGi511145.b3083.

Proteomic databases

PaxDbiP64578.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76118; AAC76118; b3083.
BAE77133; BAE77133; BAE77133.
GeneIDi947591.
KEGGiecj:JW3054.
eco:b3083.
PATRICi32121584. VBIEscCol129921_3178.

Organism-specific databases

EchoBASEiEB2584.
EcoGeneiEG12725. higB.

Phylogenomic databases

eggNOGiENOG4105R48. Bacteria.
COG4680. LUCA.
HOGENOMiHOG000026126.
InParanoidiP64578.
KOiK19166.
OMAiTIFPSAD.
OrthoDBiEOG6G20P2.

Enzyme and pathway databases

BioCyciEcoCyc:G7602-MONOMER.
ECOL316407:JW3054-MONOMER.
MetaCyc:G7602-MONOMER.

Miscellaneous databases

PROiP64578.

Family and domain databases

InterProiIPR018669. Toxin_HigB_put.
[Graphical view]
PfamiPF09907. HigB_toxin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Three new RelE-homologous mRNA interferases of Escherichia coli differentially induced by environmental stresses."
    Christensen-Dalsgaard M., Jorgensen M.G., Gerdes K.
    Mol. Microbiol. 75:333-348(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS AN MRNA INTERFERASE, INDUCTION, OPERON STRUCTURE.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiHIGB_ECOLI
AccessioniPrimary (citable) accession number: P64578
Secondary accession number(s): P42595, Q2M9C3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: July 6, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.