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Protein

7-carboxy-7-deazaguanine synthase

Gene

queE

Organism
Shigella flexneri
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.UniRule annotation

Catalytic activityi

6-carboxy-5,6,7,8-tetrahydropterin = 7-carboxy-7-carbaguanine + NH3.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • S-adenosyl-L-methionineUniRule annotationNote: Binds 1 S-adenosyl-L-methionine per subunit.UniRule annotation
  • Mg2+UniRule annotation

Pathwayi: 7-cyano-7-deazaguanine biosynthesis

This protein is involved in the pathway 7-cyano-7-deazaguanine biosynthesis, which is part of Purine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway 7-cyano-7-deazaguanine biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei27SubstrateUniRule annotation1
Metal bindingi31Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi35Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi38Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi40MagnesiumUniRule annotation1
Binding sitei92SubstrateUniRule annotation1
Binding sitei94S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processQueuosine biosynthesis
Ligand4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00391

Names & Taxonomyi

Protein namesi
Recommended name:
7-carboxy-7-deazaguanine synthaseUniRule annotation (EC:4.3.99.3UniRule annotation)
Short name:
CDG synthaseUniRule annotation
Alternative name(s):
Queuosine biosynthesis protein QueEUniRule annotation
Gene namesi
Name:queEUniRule annotation
Ordered Locus Names:SF2790, S2984
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi
  • UP000002673 Componenti: Chromosome
  • UP000001006 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001693181 – 2237-carboxy-7-deazaguanine synthaseAdd BLAST223

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP64556
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni12 – 14Substrate bindingUniRule annotation3
Regioni136 – 138S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the radical SAM superfamily. 7-carboxy-7-deazaguanine synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105EIZ Bacteria
COG0602 LUCA
HOGENOMiHOG000266143
KOiK10026
OMAiCDVKESW

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00917 QueE, 1 hit
InterProiView protein in InterPro
IPR024924 7-CO-7-deazaguanine_synth-like
IPR013785 Aldolase_TIM
IPR007197 rSAM
IPR027609 rSAM_QueE_Proteobac
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF000370 QueE, 1 hit
SFLDiSFLDS00029 Radical_SAM, 1 hit
TIGRFAMsiTIGR04322 rSAM_QueE_Ecoli, 1 hit

Sequencei

Sequence statusi: Complete.

P64556-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQYPINEMFQ TLQGEGYFTG VPAIFIRLQG CPVGCAWCDT KHTWEKLEDR
60 70 80 90 100
EVSLFSILAK TKESDKWGAA SSEDLLAVIG RQGYTARHVV ITGGEPCIHD
110 120 130 140 150
LLPLTDLLEK NGFSCQIETS GTHEVRCTPN TWVTVSPKLN MRGGYEVLSQ
160 170 180 190 200
ALERANEIKH PVGRVRDIEA LDELLATLTD DKPRVIALQP ISQKDDATRL
210 220
CIETCIARNW RLSMQTHKYL NIA
Length:223
Mass (Da):25,030
Last modified:October 11, 2004 - v1
Checksum:iA717AAF18F2A5D70
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA Translation: AAN44278.1
AE014073 Genomic DNA Translation: AAP18104.1
RefSeqiNP_708571.1, NC_004337.2
WP_001199973.1, NZ_NIYR01000069.1

Genome annotation databases

EnsemblBacteriaiAAN44278; AAN44278; SF2790
AAP18104; AAP18104; S2984
GeneIDi1026921
KEGGisfl:SF2790
sfx:S2984
PATRICifig|198214.7.peg.3321

Similar proteinsi

Entry informationi

Entry nameiQUEE_SHIFL
AccessioniPrimary (citable) accession number: P64556
Secondary accession number(s): P55139
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: May 23, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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