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Protein

7-carboxy-7-deazaguanine synthase

Gene

queE

Organism
Shigella flexneri
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.UniRule annotation

Catalytic activityi

6-carboxy-5,6,7,8-tetrahydropterin = 7-carboxy-7-carbaguanine + NH3.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • S-adenosyl-L-methionineUniRule annotationNote: Binds 1 S-adenosyl-L-methionine per subunit.UniRule annotation
  • Mg2+UniRule annotation

Pathwayi: 7-cyano-7-deazaguanine biosynthesis

This protein is involved in the pathway 7-cyano-7-deazaguanine biosynthesis, which is part of Purine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway 7-cyano-7-deazaguanine biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei27 – 271SubstrateUniRule annotation
Metal bindingi31 – 311Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi35 – 351Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi38 – 381Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi40 – 401MagnesiumUniRule annotation
Binding sitei92 – 921SubstrateUniRule annotation
Binding sitei94 – 941S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Queuosine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00391.

Names & Taxonomyi

Protein namesi
Recommended name:
7-carboxy-7-deazaguanine synthaseUniRule annotation (EC:4.3.99.3UniRule annotation)
Short name:
CDG synthaseUniRule annotation
Alternative name(s):
Queuosine biosynthesis protein QueEUniRule annotation
Gene namesi
Name:queEUniRule annotation
Ordered Locus Names:SF2790, S2984
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
Proteomesi
  • UP000002673 Componenti: Chromosome
  • UP000001006 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2232237-carboxy-7-deazaguanine synthasePRO_0000169318Add
BLAST

Proteomic databases

PaxDbiP64556.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi198214.SF2790.

Structurei

3D structure databases

ProteinModelPortaliP64556.
SMRiP64556. Positions 3-222.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni12 – 143Substrate bindingUniRule annotation
Regioni136 – 1383S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the radical SAM superfamily. 7-carboxy-7-deazaguanine synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105EIZ. Bacteria.
COG0602. LUCA.
HOGENOMiHOG000266143.
KOiK10026.
OMAiPCIHDLT.
OrthoDBiEOG6RJV40.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00917. QueE.
InterProiIPR024924. 7-CO-7-deazaguanine_synth-like.
IPR013785. Aldolase_TIM.
IPR007197. rSAM.
IPR027609. rSAM_QueE_Proteobac.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF000370. QueE. 1 hit.
TIGRFAMsiTIGR04322. rSAM_QueE_Ecoli. 1 hit.

Sequencei

Sequence statusi: Complete.

P64556-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQYPINEMFQ TLQGEGYFTG VPAIFIRLQG CPVGCAWCDT KHTWEKLEDR
60 70 80 90 100
EVSLFSILAK TKESDKWGAA SSEDLLAVIG RQGYTARHVV ITGGEPCIHD
110 120 130 140 150
LLPLTDLLEK NGFSCQIETS GTHEVRCTPN TWVTVSPKLN MRGGYEVLSQ
160 170 180 190 200
ALERANEIKH PVGRVRDIEA LDELLATLTD DKPRVIALQP ISQKDDATRL
210 220
CIETCIARNW RLSMQTHKYL NIA
Length:223
Mass (Da):25,030
Last modified:October 11, 2004 - v1
Checksum:iA717AAF18F2A5D70
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA. Translation: AAN44278.1.
AE014073 Genomic DNA. Translation: AAP18104.1.
RefSeqiNP_708571.1. NC_004337.2.
WP_001199973.1. NZ_LM651928.1.

Genome annotation databases

EnsemblBacteriaiAAN44278; AAN44278; SF2790.
AAP18104; AAP18104; S2984.
GeneIDi1026921.
KEGGisfl:SF2790.
sft:NCTC1_03070.
sfx:S2984.
PATRICi18707643. VBIShiFle31049_3276.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA. Translation: AAN44278.1.
AE014073 Genomic DNA. Translation: AAP18104.1.
RefSeqiNP_708571.1. NC_004337.2.
WP_001199973.1. NZ_LM651928.1.

3D structure databases

ProteinModelPortaliP64556.
SMRiP64556. Positions 3-222.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198214.SF2790.

Proteomic databases

PaxDbiP64556.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN44278; AAN44278; SF2790.
AAP18104; AAP18104; S2984.
GeneIDi1026921.
KEGGisfl:SF2790.
sft:NCTC1_03070.
sfx:S2984.
PATRICi18707643. VBIShiFle31049_3276.

Phylogenomic databases

eggNOGiENOG4105EIZ. Bacteria.
COG0602. LUCA.
HOGENOMiHOG000266143.
KOiK10026.
OMAiPCIHDLT.
OrthoDBiEOG6RJV40.

Enzyme and pathway databases

UniPathwayiUPA00391.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00917. QueE.
InterProiIPR024924. 7-CO-7-deazaguanine_synth-like.
IPR013785. Aldolase_TIM.
IPR007197. rSAM.
IPR027609. rSAM_QueE_Proteobac.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF000370. QueE. 1 hit.
TIGRFAMsiTIGR04322. rSAM_QueE_Ecoli. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
    Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y.
    , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
    Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 301 / Serotype 2a.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700930 / 2457T / Serotype 2a.

Entry informationi

Entry nameiQUEE_SHIFL
AccessioniPrimary (citable) accession number: P64556
Secondary accession number(s): P55139
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: July 6, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.