Reviewed, UniProtKB/Swiss-Prot P64554 (YGCF_ECOLI)

Names and origin

Protein names

Recommended name:
    Uncharacterized protein ygcF

Gene names

Name:	ygcF
Ordered Locus Names:	b2777, JW2748

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	223 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine Potential.
Sequence similarities	To H.influenzae HI1189.

Ontologies

Keywords
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW catalytic activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
rplB	P60422	1	EBI-1126335,EBI-543515

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 223	223	Uncharacterized protein ygcF		PRO_0000169316
Sites
	Metal binding	31	1	Iron-sulfur (4Fe-4S-S-AdoMet) Potential
	Metal binding	35	1	Iron-sulfur (4Fe-4S-S-AdoMet) Potential
	Metal binding	38	1	Iron-sulfur (4Fe-4S-S-AdoMet) Potential

Sequences

	Sequence		Length	Mass (Da)
	P64554-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: A717AAF18F2A5D70	FASTA	223	25,030
	10 20 30 40 50 60 MQYPINEMFQ TLQGEGYFTG VPAIFIRLQG CPVGCAWCDT KHTWEKLEDR EVSLFSILAK 70 80 90 100 110 120 TKESDKWGAA SSEDLLAVIG RQGYTARHVV ITGGEPCIHD LLPLTDLLEK NGFSCQIETS 130 140 150 160 170 180 GTHEVRCTPN TWVTVSPKLN MRGGYEVLSQ ALERANEIKH PVGRVRDIEA LDELLATLTD 190 200 210 220 DKPRVIALQP ISQKDDATRL CIETCIARNW RLSMQTHKYL NIA

References

[1]

"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.

[2]

"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U29580 Genomic DNA. Translation: AAA69287.1. U00096 Genomic DNA. Translation: AAC75819.1. AP009048 Genomic DNA. Translation: BAE76851.1.
PIR	E65059.
RefSeq	AP_003343.1. NP_417257.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P64554. 2 interactions.
STRING	P64554.
Genome annotation databases
GeneID	947527.
GenomeReviews	Gene locus JW2748 in contig AP009048_GR. Gene locus b2777 in contig U00096_GR.
KEGG	ecj:JW2748. eco:b2777.
Organism-specific databases
EchoBASE	EB2849.
EcoGene	EG13034. ygcF.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG507714.
OMA	TICLQPI.
Enzyme and pathway databases
BioCyc	EcoCyc:G7443-MON. ECOL168927:B2777-MON.
Gene expression databases
Genevestigator	P64554.
Family and domain databases
InterPro	IPR007197. Radical_SAM. [Graphical view]
Pfam	PF04055. Radical_SAM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YGCF_ECOLI

Accession

Primary (citable) accession number: P64554
Secondary accession number(s): P55139, Q2MA55

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	December 15, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)