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Protein

7-carboxy-7-deazaguanine synthase

Gene

queE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.UniRule annotation

Catalytic activityi

6-carboxy-5,6,7,8-tetrahydropterin = 7-carboxy-7-carbaguanine + NH3.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • S-adenosyl-L-methionineUniRule annotationNote: Binds 1 S-adenosyl-L-methionine per subunit.UniRule annotation
  • Mg2+UniRule annotation

Pathwayi: 7-cyano-7-deazaguanine biosynthesis

This protein is involved in the pathway 7-cyano-7-deazaguanine biosynthesis, which is part of Purine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway 7-cyano-7-deazaguanine biosynthesis and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei27SubstrateUniRule annotation1
Metal bindingi31Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi35Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi38Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi40MagnesiumUniRule annotation1
Binding sitei92SubstrateUniRule annotation1
Binding sitei94S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processQueuosine biosynthesis
Ligand4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:G7443-MONOMER
UniPathwayiUPA00391

Names & Taxonomyi

Protein namesi
Recommended name:
7-carboxy-7-deazaguanine synthaseUniRule annotation (EC:4.3.99.3UniRule annotation)
Short name:
CDG synthaseUniRule annotation
Alternative name(s):
Queuosine biosynthesis protein QueEUniRule annotation
Gene namesi
Name:queEUniRule annotation
Synonyms:ygcF
Ordered Locus Names:b2777, JW2748
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi

Organism-specific databases

EcoGeneiEG13034 queE

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001693161 – 2237-carboxy-7-deazaguanine synthaseAdd BLAST223

Proteomic databases

PaxDbiP64554
PRIDEiP64554

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

BioGridi4262295, 24 interactors
DIPiDIP-48192N
IntActiP64554, 3 interactors
STRINGi316385.ECDH10B_2944

Structurei

3D structure databases

ProteinModelPortaliP64554
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni12 – 14Substrate bindingUniRule annotation3
Regioni136 – 138S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the radical SAM superfamily. 7-carboxy-7-deazaguanine synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105EIZ Bacteria
COG0602 LUCA
HOGENOMiHOG000266143
InParanoidiP64554
KOiK10026
OMAiCDVKESW
PhylomeDBiP64554

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00917 QueE, 1 hit
InterProiView protein in InterPro
IPR024924 7-CO-7-deazaguanine_synth-like
IPR013785 Aldolase_TIM
IPR007197 rSAM
IPR027609 rSAM_QueE_Proteobac
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF000370 QueE, 1 hit
SFLDiSFLDS00029 Radical_SAM, 1 hit
TIGRFAMsiTIGR04322 rSAM_QueE_Ecoli, 1 hit

Sequencei

Sequence statusi: Complete.

P64554-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQYPINEMFQ TLQGEGYFTG VPAIFIRLQG CPVGCAWCDT KHTWEKLEDR
60 70 80 90 100
EVSLFSILAK TKESDKWGAA SSEDLLAVIG RQGYTARHVV ITGGEPCIHD
110 120 130 140 150
LLPLTDLLEK NGFSCQIETS GTHEVRCTPN TWVTVSPKLN MRGGYEVLSQ
160 170 180 190 200
ALERANEIKH PVGRVRDIEA LDELLATLTD DKPRVIALQP ISQKDDATRL
210 220
CIETCIARNW RLSMQTHKYL NIA
Length:223
Mass (Da):25,030
Last modified:October 11, 2004 - v1
Checksum:iA717AAF18F2A5D70
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29580 Genomic DNA Translation: AAA69287.1
U00096 Genomic DNA Translation: AAC75819.1
AP009048 Genomic DNA Translation: BAE76851.1
PIRiE65059
RefSeqiNP_417257.1, NC_000913.3
WP_001199973.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75819; AAC75819; b2777
BAE76851; BAE76851; BAE76851
GeneIDi947527
KEGGiecj:JW2748
eco:b2777
PATRICifig|1411691.4.peg.3959

Entry informationi

Entry nameiQUEE_ECOLI
AccessioniPrimary (citable) accession number: P64554
Secondary accession number(s): P55139, Q2MA55
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: March 28, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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