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Protein

L-alanine exporter AlaE

Gene

alaE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Exports L-alanine.UniRule annotation1 Publication

GO - Molecular functioni

  • L-amino acid efflux transmembrane transporter activity Source: EcoCyc
  • solute:proton antiporter activity Source: EcoCyc

GO - Biological processi

  • L-alanine transport Source: EcoCyc

Keywordsi

Biological processAmino-acid transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:G7399-MONOMER
MetaCyc:G7399-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
L-alanine exporter AlaEUniRule annotation
Gene namesi
Name:alaEUniRule annotation
Synonyms:ygaW
Ordered Locus Names:b2670, JW2645
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13525 alaE

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei16 – 36HelicalUniRule annotationAdd BLAST21
Transmembranei46 – 66HelicalUniRule annotationAdd BLAST21
Transmembranei85 – 105HelicalUniRule annotationAdd BLAST21
Transmembranei112 – 132HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutants show a significant accumulation of intracellular L-alanine and a reduction in the L-alanine export rate.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001693021 – 149L-alanine exporter AlaEAdd BLAST149

Proteomic databases

PaxDbiP64550
PRIDEiP64550

Expressioni

Inductioni

Induced in the presence of L-alanyl-L-alanine dipeptides.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4261134, 9 interactors
STRINGi316385.ECDH10B_2837

Structurei

3D structure databases

ProteinModelPortaliP64550
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AlaE exporter family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108RNS Bacteria
ENOG4111J5P LUCA
HOGENOMiHOG000123992
OMAiADWHQIA

Family and domain databases

HAMAPiMF_00914 L_Ala_exporter, 1 hit
InterProiView protein in InterPro
IPR010574 Ala_export_AlaE
PfamiView protein in Pfam
PF06610 AlaE, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD090021 DUF1144, 1 hit

Sequencei

Sequence statusi: Complete.

P64550-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSPQSRLRH AVADTFAMVV YCSVVNMCIE VFLSGMSFEQ SFYSRLVAIP
60 70 80 90 100
VNILIAWPYG MYRDLFMRAA RKVSPSGWIK NLADILAYVT FQSPVYVAIL
110 120 130 140
LVVGADWHQI MAAVSSNIVV SMLMGAVYGY FLDYCRRLFK VSRYQQVKA
Length:149
Mass (Da):16,949
Last modified:October 11, 2004 - v1
Checksum:i0B1D31F498DBE4BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75717.1
AP009048 Genomic DNA Translation: BAE76781.1
PIRiG65046
RefSeqiNP_417156.1, NC_000913.3
WP_000492656.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75717; AAC75717; b2670
BAE76781; BAE76781; BAE76781
GeneIDi947147
KEGGiecj:JW2645
eco:b2670
PATRICifig|1411691.4.peg.4071

Similar proteinsi

Entry informationi

Entry nameiALAE_ECOLI
AccessioniPrimary (citable) accession number: P64550
Secondary accession number(s): P76626, Q2MAC5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: March 28, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health