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Protein

L-alanine exporter AlaE

Gene

alaE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Exports L-alanine.UniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:G7399-MONOMER.
ECOL316407:JW2645-MONOMER.
MetaCyc:G7399-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
L-alanine exporter AlaEUniRule annotation
Gene namesi
Name:alaEUniRule annotation
Synonyms:ygaW
Ordered Locus Names:b2670, JW2645
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13525. alaE.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei16 – 3621HelicalUniRule annotationAdd
BLAST
Transmembranei46 – 6621HelicalUniRule annotationAdd
BLAST
Transmembranei85 – 10521HelicalUniRule annotationAdd
BLAST
Transmembranei112 – 13221HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: EcoCyc
  • integral component of plasma membrane Source: UniProtKB-HAMAP
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutants show a significant accumulation of intracellular L-alanine and a reduction in the L-alanine export rate.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 149149L-alanine exporter AlaEPRO_0000169302Add
BLAST

Proteomic databases

PaxDbiP64550.
PRIDEiP64550.

Expressioni

Inductioni

Induced in the presence of L-alanyl-L-alanine dipeptides.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4261134. 9 interactions.
STRINGi511145.b2670.

Structurei

3D structure databases

ProteinModelPortaliP64550.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AlaE exporter family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108RNS. Bacteria.
ENOG4111J5P. LUCA.
HOGENOMiHOG000123992.
OMAiADWHQIA.
OrthoDBiEOG647V0P.

Family and domain databases

HAMAPiMF_00914. L_Ala_exporter.
InterProiIPR010574. Ala_export_AlaE.
[Graphical view]
PfamiPF06610. AlaE. 1 hit.
[Graphical view]
ProDomiPD090021. DUF1144. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

P64550-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSPQSRLRH AVADTFAMVV YCSVVNMCIE VFLSGMSFEQ SFYSRLVAIP
60 70 80 90 100
VNILIAWPYG MYRDLFMRAA RKVSPSGWIK NLADILAYVT FQSPVYVAIL
110 120 130 140
LVVGADWHQI MAAVSSNIVV SMLMGAVYGY FLDYCRRLFK VSRYQQVKA
Length:149
Mass (Da):16,949
Last modified:October 11, 2004 - v1
Checksum:i0B1D31F498DBE4BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75717.1.
AP009048 Genomic DNA. Translation: BAE76781.1.
PIRiG65046.
RefSeqiNP_417156.1. NC_000913.3.
WP_000492656.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75717; AAC75717; b2670.
BAE76781; BAE76781; BAE76781.
GeneIDi947147.
KEGGiecj:JW2645.
eco:b2670.
PATRICi32120730. VBIEscCol129921_2762.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75717.1.
AP009048 Genomic DNA. Translation: BAE76781.1.
PIRiG65046.
RefSeqiNP_417156.1. NC_000913.3.
WP_000492656.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP64550.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261134. 9 interactions.
STRINGi511145.b2670.

Proteomic databases

PaxDbiP64550.
PRIDEiP64550.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75717; AAC75717; b2670.
BAE76781; BAE76781; BAE76781.
GeneIDi947147.
KEGGiecj:JW2645.
eco:b2670.
PATRICi32120730. VBIEscCol129921_2762.

Organism-specific databases

EchoBASEiEB3297.
EcoGeneiEG13525. alaE.

Phylogenomic databases

eggNOGiENOG4108RNS. Bacteria.
ENOG4111J5P. LUCA.
HOGENOMiHOG000123992.
OMAiADWHQIA.
OrthoDBiEOG647V0P.

Enzyme and pathway databases

BioCyciEcoCyc:G7399-MONOMER.
ECOL316407:JW2645-MONOMER.
MetaCyc:G7399-MONOMER.

Miscellaneous databases

PROiP64550.

Family and domain databases

HAMAPiMF_00914. L_Ala_exporter.
InterProiIPR010574. Ala_export_AlaE.
[Graphical view]
PfamiPF06610. AlaE. 1 hit.
[Graphical view]
ProDomiPD090021. DUF1144. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Inducible L-alanine exporter encoded by the novel gene ygaW (alaE) in Escherichia coli."
    Hori H., Yoneyama H., Tobe R., Ando T., Isogai E., Katsumata R.
    Appl. Environ. Microbiol. 77:4027-4034(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN ALANINE EXPORT, SUBCELLULAR LOCATION, INDUCTION, DISRUPTION PHENOTYPE, GENE NAME.
    Strain: K12.

Entry informationi

Entry nameiALAE_ECOLI
AccessioniPrimary (citable) accession number: P64550
Secondary accession number(s): P76626, Q2MAC5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: January 20, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.