P64534 (RCNB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nickel/cobalt homeostasis protein RcnB

Gene names

Name:	rcnB
Synonyms:	yohN
Ordered Locus Names:	b2107, JW5346

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	112 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Influences nickel and cobalt homeostasis. May act by modulating RcnA-mediated export of these ions to avoid excess efflux. Not involved in nickel import and does not bind nickel or cobalt ions directly. Ref.3
Subcellular location	Periplasm Ref.3.
Induction	Repressed by RcnR. Induced by nickel and cobalt. Ref.3
Disruption phenotype	Mutants show reduced intracellular nickel or cobalt levels. Ref.3
Sequence similarities	Belongs to the RcnB family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	response to DNA damage stimulus Inferred from expression pattern PubMed 11967071. Source: EcoliWiki response to cobalt ion Inferred from expression pattern Ref.3. Source: EcoCyc response to nickel cation Inferred from expression pattern Ref.3. Source: EcoCyc
Cellular_component	outer membrane-bounded periplasmic space Inferred from direct assay Ref.3. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Ref.3
	Chain	26 – 112	87	Nickel/cobalt homeostasis protein RcnB		PRO_0000013872

Sequences

	Sequence		Length	Mass (Da)
	P64534 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: C79685089608624F	FASTA	112	12,466
	10 20 30 40 50 60 MTIKNKMLLG ALLLVTSAAW AAPATAGSTN TSGISKYELS SFIADFKHFK PGDTVPEMYR 70 80 90 100 110 TDEYNIKQWQ LRNLPAPDAG THWTYMGGAY VLISDTDGKI IKAYDGEIFY HR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"RcnB is a periplasmic protein essential for maintaining intracellular Ni and Co concentrations in Escherichia coli." Bleriot C., Effantin G., Lagarde F., Mandrand-Berthelot M.A., Rodrigue A. J. Bacteriol. 193:3785-3793(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 26-31, FUNCTION, SUBCELLULAR LOCATION, INDUCTION, DISRUPTION PHENOTYPE. Strain: K12.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC75168.2. AP009048 Genomic DNA. Translation: BAE76587.1.
RefSeq	NP_416610.4. NC_000913.2. YP_490347.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P64534.
SMR	P64534. Positions 32-112.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48070N.
STRING	511145.b2107.
Protein family/group databases
TCDB	2.A.52.2.1. Ni2+-Co2+ transporter (NiCoT) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75168; AAC75168; b2107. BAE76587; BAE76587; BAE76587.
GeneID	12931439. 949080.
KEGG	ecj:Y75_p2069. eco:b2107.
PATRIC	32119547. VBIEscCol129921_2184.
Organism-specific databases
EchoBASE	EB3825.
EcoGene	EG14072. rcnB.
Phylogenomic databases
eggNOG	COG5455.
HOGENOM	HOG000122121.
OMA	NEDAFAT.
ProtClustDB	CLSK880293.
Enzyme and pathway databases
BioCyc	EcoCyc:G7139-MONOMER. ECOL316407:JW5346-MONOMER.
Gene expression databases
Genevestigator	P64534.
Family and domain databases
InterPro	IPR024572. DUF3315. [Graphical view]
Pfam	PF11776. DUF3315. 1 hit. [Graphical view]
ProDom	PD456999. PD456999. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

RCNB_ECOLI

Accession

Primary (citable) accession number: P64534
Secondary accession number(s): P76426, Q2MAW9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program