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Protein

Nickel/cobalt homeostasis protein RcnB

Gene

rcnB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Influences nickel and cobalt homeostasis. May act by modulating RcnA-mediated export of these ions to avoid excess efflux. Not involved in nickel import and does not bind nickel or cobalt ions directly.1 Publication

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • response to cobalt ion Source: EcoCyc
  • response to nickel cation Source: EcoCyc
  • response to peptide Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:G7139-MONOMER.
ECOL316407:JW5346-MONOMER.

Protein family/group databases

TCDBi2.A.113.1.1. 2.a.113. the nickel/cobalt transporter (nico) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Nickel/cobalt homeostasis protein RcnB
Gene namesi
Name:rcnB
Synonyms:yohN
Ordered Locus Names:b2107, JW5346
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14072. rcnB.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • outer membrane-bounded periplasmic space Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Disruption phenotypei

Mutants show reduced intracellular nickel or cobalt levels.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 25251 PublicationAdd
BLAST
Chaini26 – 11287Nickel/cobalt homeostasis protein RcnBPRO_0000013872Add
BLAST

Proteomic databases

PaxDbiP64534.

Expressioni

Inductioni

Repressed by RcnR. Induced by nickel and cobalt.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4260433. 2 interactions.
DIPiDIP-48070N.
IntActiP64534. 5 interactions.
STRINGi511145.b2107.

Structurei

3D structure databases

ProteinModelPortaliP64534.
SMRiP64534. Positions 32-112.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RcnB family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108Z1S. Bacteria.
COG5455. LUCA.
HOGENOMiHOG000122121.
OMAiILKSAYS.
OrthoDBiEOG69GZRG.

Family and domain databases

InterProiIPR024572. RcnB.
[Graphical view]
PfamiPF11776. RcnB. 1 hit.
[Graphical view]
ProDomiPD456999. PD456999. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P64534-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIKNKMLLG ALLLVTSAAW AAPATAGSTN TSGISKYELS SFIADFKHFK
60 70 80 90 100
PGDTVPEMYR TDEYNIKQWQ LRNLPAPDAG THWTYMGGAY VLISDTDGKI
110
IKAYDGEIFY HR
Length:112
Mass (Da):12,466
Last modified:October 11, 2004 - v1
Checksum:iC79685089608624F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75168.2.
AP009048 Genomic DNA. Translation: BAE76587.1.
RefSeqiNP_416610.4. NC_000913.3.
WP_000153067.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75168; AAC75168; b2107.
BAE76587; BAE76587; BAE76587.
GeneIDi949080.
KEGGiecj:JW5346.
eco:b2107.
PATRICi32119547. VBIEscCol129921_2184.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75168.2.
AP009048 Genomic DNA. Translation: BAE76587.1.
RefSeqiNP_416610.4. NC_000913.3.
WP_000153067.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP64534.
SMRiP64534. Positions 32-112.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260433. 2 interactions.
DIPiDIP-48070N.
IntActiP64534. 5 interactions.
STRINGi511145.b2107.

Protein family/group databases

TCDBi2.A.113.1.1. 2.a.113. the nickel/cobalt transporter (nico) family.

Proteomic databases

PaxDbiP64534.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75168; AAC75168; b2107.
BAE76587; BAE76587; BAE76587.
GeneIDi949080.
KEGGiecj:JW5346.
eco:b2107.
PATRICi32119547. VBIEscCol129921_2184.

Organism-specific databases

EchoBASEiEB3825.
EcoGeneiEG14072. rcnB.

Phylogenomic databases

eggNOGiENOG4108Z1S. Bacteria.
COG5455. LUCA.
HOGENOMiHOG000122121.
OMAiILKSAYS.
OrthoDBiEOG69GZRG.

Enzyme and pathway databases

BioCyciEcoCyc:G7139-MONOMER.
ECOL316407:JW5346-MONOMER.

Miscellaneous databases

PROiP64534.

Family and domain databases

InterProiIPR024572. RcnB.
[Graphical view]
PfamiPF11776. RcnB. 1 hit.
[Graphical view]
ProDomiPD456999. PD456999. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "RcnB is a periplasmic protein essential for maintaining intracellular Ni and Co concentrations in Escherichia coli."
    Bleriot C., Effantin G., Lagarde F., Mandrand-Berthelot M.A., Rodrigue A.
    J. Bacteriol. 193:3785-3793(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 26-31, FUNCTION, SUBCELLULAR LOCATION, INDUCTION, DISRUPTION PHENOTYPE.
    Strain: K12.

Entry informationi

Entry nameiRCNB_ECOLI
AccessioniPrimary (citable) accession number: P64534
Secondary accession number(s): P76426, Q2MAW9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: January 20, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.