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Protein

Cytoskeleton-binding toxin CbtA

Gene

cbtA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Toxic component of a type IV toxin-antitoxin (TA) module. Interacts with cytoskeletal proteins FtsZ and MreB; inhibits FtsZ GTP-dependent polymerization and GTPase activity as well as MreB ATP-dependent polymerization. Binds to both the N- and C-terminus of FtsZ, likely blocking its polymerization. Overexpression results in inhibition of growth in liquid cultures and decrease in colony formation; these effects are overcome by concomitant expression of antitoxin CbeA (YeeU). In other experiments after 6 hours cells are lemon-shaped and by 24 hours those that have not lysed are spherical with diminished polar regions. Toxic effects are neutralized by cognate antitoxin CbeA, although there is no direct interaction between the 2 proteins.3 Publications

GO - Molecular functioni

  • cytoskeletal protein binding Source: EcoCyc

GO - Biological processi

  • programmed cell death Source: EcoCyc
  • regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Toxin

Keywords - Biological processi

Cell shape

Enzyme and pathway databases

BioCyciEcoCyc:G7085-MONOMER.
ECOL316407:JW1987-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoskeleton-binding toxin CbtA1 Publication
Alternative name(s):
Toxin CbtA1 Publication
Toxin YeeV
Gene namesi
Name:cbtA1 Publication
Synonyms:yeeV
Ordered Locus Names:b2005, JW1987
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13389. cbtA.

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1 – 15Missing : No longer toxic, cells become lemon-shaped more quickly than wild-type on overexpression. 1 PublicationAdd BLAST15

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001691161 – 124Cytoskeleton-binding toxin CbtAAdd BLAST124

Proteomic databases

PaxDbiP64524.
PRIDEiP64524.

Interactioni

Subunit structurei

Interacts with FtsZ and MreB.1 Publication

GO - Molecular functioni

  • cytoskeletal protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4261508. 8 interactors.
IntActiP64524. 6 interactors.
STRINGi511145.b2005.

Structurei

3D structure databases

ProteinModelPortaliP64524.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 73Remains toxic, toxin less stabile; cells filament, do not interact with MreB but probably still interact with FtsZAdd BLAST73
Regioni74 – 124Required for interaction with MreBAdd BLAST51

Domaini

The N-terminal 15 residues are required for interaction with both FtsZ and MreB, while the C-terminal 63 residues are required for interaction with MreB.1 Publication

Sequence similaritiesi

Belongs to the CbtA/YkfI/YpjF toxin family.Curated

Phylogenomic databases

HOGENOMiHOG000124769.
KOiK18837.
PhylomeDBiP64524.

Family and domain databases

InterProiIPR009610. CbtA_toxin.
[Graphical view]
PfamiPF06755. CbtA_toxin. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P64524-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTLPVLPGQ AASSRPSPVE IWQILLSRLL DQHYGLTLND TPFADERVIE
60 70 80 90 100
QHIEAGISLC DAVNFLVEKY ALVRTDQPGF SACTRSQLIN SIDILRARRA
110 120
TGLMTRDNYR TVNNITLGKY PEAK
Length:124
Mass (Da):13,899
Last modified:October 11, 2004 - v1
Checksum:i4327D21EC2A8159A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75066.1.
AP009048 Genomic DNA. Translation: BAE76565.1.
PIRiD64965.
RefSeqiNP_416509.1. NC_000913.3.
WP_000854814.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75066; AAC75066; b2005.
BAE76565; BAE76565; BAE76565.
GeneIDi946534.
KEGGiecj:JW1987.
eco:b2005.
PATRICi32119343. VBIEscCol129921_2081.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC75066.1.
AP009048 Genomic DNA. Translation: BAE76565.1.
PIRiD64965.
RefSeqiNP_416509.1. NC_000913.3.
WP_000854814.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP64524.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261508. 8 interactors.
IntActiP64524. 6 interactors.
STRINGi511145.b2005.

Proteomic databases

PaxDbiP64524.
PRIDEiP64524.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75066; AAC75066; b2005.
BAE76565; BAE76565; BAE76565.
GeneIDi946534.
KEGGiecj:JW1987.
eco:b2005.
PATRICi32119343. VBIEscCol129921_2081.

Organism-specific databases

EchoBASEiEB3170.
EcoGeneiEG13389. cbtA.

Phylogenomic databases

HOGENOMiHOG000124769.
KOiK18837.
PhylomeDBiP64524.

Enzyme and pathway databases

BioCyciEcoCyc:G7085-MONOMER.
ECOL316407:JW1987-MONOMER.

Miscellaneous databases

PROiP64524.

Family and domain databases

InterProiIPR009610. CbtA_toxin.
[Graphical view]
PfamiPF06755. CbtA_toxin. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCBTA_ECOLI
AccessioniPrimary (citable) accession number: P64524
Secondary accession number(s): P76365, Q2MAZ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: November 2, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.