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Protein

PhoP/PhoQ regulator MgrB

Gene

mgrB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Represses PhoP/PhoQ signaling, possibly by binding to the periplasmic domain of PhoQ, altering its activity and that of downstream effector PhoP. PhoP-regulated transcription is redox-sensitive, being activated when the periplasm becomes more reducing (deletion of dsbA/dsbB, treatment with dithiothreitol). MgrB acts between DsbA/DsbB and PhoP/PhoQ in this pathway; the 2 periplasmic Cys residues of MgrB are required for its action on PhoQ, and thus PhoP.2 Publications

GO - Biological processi

  • cellular response to magnesium ion Source: EcoCyc
  • negative regulation of molecular function Source: EcoCyc
  • negative regulation of phosphorelay signal transduction system Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • response to acidic pH Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:G7002-MONOMER.
ECOL316407:JW1815-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
PhoP/PhoQ regulator MgrBUniRule annotation
Gene namesi
Name:mgrBUniRule annotation
Synonyms:yobG
Ordered Locus Names:b1826, JW1815
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14383. mgrB.

Subcellular locationi

  • Cell inner membrane 1 Publication; Single-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 5CytoplasmicSequence analysis5
Transmembranei6 – 26HelicalUniRule annotationAdd BLAST21
Topological domaini27 – 47Periplasmic1 PublicationAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Induction of genes regulated by PhoP, not suppressed by a dsbA deletion or 50 µM CuSO4.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi16C → A: Still represses PhoP-regulated transcription. 1 Publication1
Mutagenesisi28C → A: No longer represses PhoP-regulated transcription. 1 Publication1
Mutagenesisi39C → A: No longer represses PhoP-regulated transcription. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001690661 – 47PhoP/PhoQ regulator MgrBAdd BLAST47

Proteomic databases

PaxDbiP64512.
PRIDEiP64512.

Expressioni

Inductioni

Induced by low extracellular levels of Mg2+ via the PhoP/PhoQ two-component regulatory system (PubMed:10464230) (Probable). In exponential phase (at protein level) (PubMed:19121005). By dithiothreitol (PubMed:22267510).Curated6 Publications

Interactioni

Subunit structurei

May form homooligomers. Probably interacts with the periplasmic domain of PhoQ.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi4261732. 6 interactors.
STRINGi511145.b1826.

Structurei

3D structure databases

ProteinModelPortaliP64512.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MgrB family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410XW9Z. LUCA.
HOGENOMiHOG000059286.

Family and domain databases

HAMAPiMF_01596. MgrB. 1 hit.
InterProiIPR020907. MgrB.
[Graphical view]
PfamiPF13998. MgrB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P64512-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40 
MKKFRWVVLV VVVLACLLLW AQVFNMMCDQ DVQFFSGICA INQFIPW
Length:47
Mass (Da):5,552
Last modified:October 11, 2004 - v1
Checksum:iB1F03878D23CDDDE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74896.1.
AP009048 Genomic DNA. Translation: BAE76539.1.
PIRiB64944.
RefSeqiNP_416340.1. NC_000913.3.
WP_000714550.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74896; AAC74896; b1826.
BAE76539; BAE76539; BAE76539.
GeneIDi946351.
KEGGiecj:JW1815.
eco:b1826.
PATRICi32118971. VBIEscCol129921_1903.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74896.1.
AP009048 Genomic DNA. Translation: BAE76539.1.
PIRiB64944.
RefSeqiNP_416340.1. NC_000913.3.
WP_000714550.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP64512.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261732. 6 interactors.
STRINGi511145.b1826.

Proteomic databases

PaxDbiP64512.
PRIDEiP64512.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74896; AAC74896; b1826.
BAE76539; BAE76539; BAE76539.
GeneIDi946351.
KEGGiecj:JW1815.
eco:b1826.
PATRICi32118971. VBIEscCol129921_1903.

Organism-specific databases

EchoBASEiEB4126.
EcoGeneiEG14383. mgrB.

Phylogenomic databases

eggNOGiENOG410XW9Z. LUCA.
HOGENOMiHOG000059286.

Enzyme and pathway databases

BioCyciEcoCyc:G7002-MONOMER.
ECOL316407:JW1815-MONOMER.

Miscellaneous databases

PROiP64512.

Family and domain databases

HAMAPiMF_01596. MgrB. 1 hit.
InterProiIPR020907. MgrB.
[Graphical view]
PfamiPF13998. MgrB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMGRB_ECOLI
AccessioniPrimary (citable) accession number: P64512
Secondary accession number(s): P76267, Q2MB17
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: November 2, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.