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Protein

OriC-binding nucleoid-associated protein

Gene

cnu

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The complex formed with H-NS binds to the specific 26-bp cnb site in the origin of replication oriC. Can complement, at least partially, the absence of the Hha protein in hha mutants.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei44 – 441Interacts with H-NS

GO - Molecular functioni

GO - Biological processi

  • negative regulation of single-species biofilm formation on inanimate substrate Source: EcoCyc
  • positive regulation of DNA-templated transcription, termination Source: EcoCyc
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6869-MONOMER.
ECOL316407:JW1617-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
OriC-binding nucleoid-associated protein
Alternative name(s):
H-NS/StpA-binding protein 2
Gene namesi
Name:cnu
Synonyms:ydgT
Ordered Locus Names:b1625, JW1617
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14303. cnu.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi44 – 441D → N: Abolishes heterocomplex formation with H-NS. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7171OriC-binding nucleoid-associated proteinPRO_0000201733Add
BLAST

Proteomic databases

PaxDbiP64467.

Interactioni

Subunit structurei

Forms complexes with both H-NS and StpA.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
hnsP0ACF82EBI-551907,EBI-544934

Protein-protein interaction databases

BioGridi4259630. 18 interactions.
DIPiDIP-48011N.
IntActiP64467. 3 interactions.
MINTiMINT-8175315.
STRINGi511145.b1625.

Structurei

Secondary structure

1
71
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 126Combined sources
Helixi17 – 2913Combined sources
Helixi33 – 5119Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JQTNMR-A1-71[»]
ProteinModelPortaliP64467.
SMRiP64467. Positions 1-57.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP64467.

Family & Domainsi

Sequence similaritiesi

Belongs to the Hha/YmoA/Cnu family.Curated

Phylogenomic databases

eggNOGiENOG4105QZ5. Bacteria.
ENOG4111UG9. LUCA.
HOGENOMiHOG000219358.
OMAiPKSVWHF.
OrthoDBiEOG6JQH6S.

Family and domain databases

Gene3Di1.20.1280.40. 1 hit.
InterProiIPR007985. Hemolysn_expr_modulating_HHA.
[Graphical view]
PfamiPF05321. HHA. 1 hit.
[Graphical view]
SUPFAMiSSF68989. SSF68989. 1 hit.

Sequencei

Sequence statusi: Complete.

P64467-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVQDYLLKF RKISSLESLE KLYDHLNYTL TDDQELINMY RAADHRRAEL
60 70
VSGGRLFDLG QVPKSVWHYV Q
Length:71
Mass (Da):8,417
Last modified:October 11, 2004 - v1
Checksum:iCE9FF2E890E78219
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY442175 Genomic DNA. Translation: AAS07631.1.
U00096 Genomic DNA. Translation: AAC74697.1.
AP009048 Genomic DNA. Translation: BAE76484.1.
PIRiC64919.
RefSeqiNP_416142.1. NC_000913.3.
WP_000217950.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74697; AAC74697; b1625.
BAE76484; BAE76484; BAE76484.
GeneIDi946152.
KEGGiecj:JW1617.
eco:b1625.
PATRICi32118552. VBIEscCol129921_1696.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY442175 Genomic DNA. Translation: AAS07631.1.
U00096 Genomic DNA. Translation: AAC74697.1.
AP009048 Genomic DNA. Translation: BAE76484.1.
PIRiC64919.
RefSeqiNP_416142.1. NC_000913.3.
WP_000217950.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2JQTNMR-A1-71[»]
ProteinModelPortaliP64467.
SMRiP64467. Positions 1-57.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259630. 18 interactions.
DIPiDIP-48011N.
IntActiP64467. 3 interactions.
MINTiMINT-8175315.
STRINGi511145.b1625.

Proteomic databases

PaxDbiP64467.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74697; AAC74697; b1625.
BAE76484; BAE76484; BAE76484.
GeneIDi946152.
KEGGiecj:JW1617.
eco:b1625.
PATRICi32118552. VBIEscCol129921_1696.

Organism-specific databases

EchoBASEiEB4050.
EcoGeneiEG14303. cnu.

Phylogenomic databases

eggNOGiENOG4105QZ5. Bacteria.
ENOG4111UG9. LUCA.
HOGENOMiHOG000219358.
OMAiPKSVWHF.
OrthoDBiEOG6JQH6S.

Enzyme and pathway databases

BioCyciEcoCyc:G6869-MONOMER.
ECOL316407:JW1617-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP64467.
PROiP64467.

Family and domain databases

Gene3Di1.20.1280.40. 1 hit.
InterProiIPR007985. Hemolysn_expr_modulating_HHA.
[Graphical view]
PfamiPF05321. HHA. 1 hit.
[Graphical view]
SUPFAMiSSF68989. SSF68989. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN BINDING TO ORIC.
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "YdgT, the Hha paralogue in Escherichia coli, forms heteromeric complexes with H-NS and StpA."
    Paytubi S., Madrid C., Forns N., Nieto J.M., Balsalobre C., Uhlin B.E., Juarez A.
    Mol. Microbiol. 54:251-263(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY FUNCTION, SUBUNIT.
  5. "Essential residues in the H-NS binding site of Hha, a co-regulator of horizontally acquired genes in Enterobacteria."
    de Alba C.F., Solorzano C., Paytubi S., Madrid C., Juarez A., Garcia J., Pons M.
    FEBS Lett. 585:1765-1770(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH H-NS, MUTAGENESIS OF ASP-44.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiCNU_ECOLI
AccessioniPrimary (citable) accession number: P64467
Secondary accession number(s): P76179, Q2MB72, Q5VJ24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: January 20, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Expression becomes maximal as cells enter the stationary phase but decreases later in the stationary phase.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.