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Protein

OriC-binding nucleoid-associated protein

Gene

cnu

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Modifies the set of genes regulated by H-NS; Hha and Cnu (YdgT) increase the number of genes bound by H-NS/StpA and may also modulate the oligomerization of the H-NS/StpA-complex on DNA (PubMed:23543115). The complex formed with H-NS binds to the specific 26-bp cnb site in the origin of replication oriC (PubMed:16199570). Can complement, at least partially, the absence of the Hha protein in hha mutants.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei44Interacts with H-NS1

GO - Molecular functioni

GO - Biological processi

  • negative regulation of single-species biofilm formation on inanimate substrate Source: EcoCyc
  • positive regulation of DNA-templated transcription, termination Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:G6869-MONOMER.
ECOL316407:JW1617-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
OriC-binding nucleoid-associated protein
Alternative name(s):
H-NS/StpA-binding protein 2
Transcription modulator YdgT
Gene namesi
Name:cnu1 Publication
Synonyms:ydgT
Ordered Locus Names:b1625, JW1617
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14303. cnu.

Pathology & Biotechi

Disruption phenotypei

At 0.3 M NaCl up-regulation of 1 gene and down-regulation of 6 genes was observed; a double cnu-hha deletion up-regulated 134 and down-regulated 5 genes, most of which are thought to have been acquired horizontally and are also up-regulated in double hns-stpA deletions (PubMed:23543115).1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi44D → N: Abolishes heterocomplex formation with H-NS. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002017331 – 71OriC-binding nucleoid-associated proteinAdd BLAST71

Proteomic databases

PaxDbiP64467.
PRIDEiP64467.

Interactioni

Subunit structurei

Forms complexes with both H-NS and StpA.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
hnsP0ACF82EBI-551907,EBI-544934

Protein-protein interaction databases

BioGridi4259630. 18 interactors.
DIPiDIP-48011N.
IntActiP64467. 3 interactors.
MINTiMINT-8175315.
STRINGi511145.b1625.

Structurei

Secondary structure

171
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 12Combined sources6
Helixi17 – 29Combined sources13
Helixi33 – 51Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JQTNMR-A1-71[»]
ProteinModelPortaliP64467.
SMRiP64467.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP64467.

Family & Domainsi

Sequence similaritiesi

Belongs to the Hha/YmoA/Cnu family.Curated

Phylogenomic databases

eggNOGiENOG4105QZ5. Bacteria.
ENOG4111UG9. LUCA.
HOGENOMiHOG000219358.
OMAiPKSVWHF.

Family and domain databases

Gene3Di1.20.1280.40. 1 hit.
InterProiIPR007985. Hemolysn_expr_modulating_HHA.
[Graphical view]
PfamiPF05321. HHA. 1 hit.
[Graphical view]
SUPFAMiSSF68989. SSF68989. 1 hit.

Sequencei

Sequence statusi: Complete.

P64467-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVQDYLLKF RKISSLESLE KLYDHLNYTL TDDQELINMY RAADHRRAEL
60 70
VSGGRLFDLG QVPKSVWHYV Q
Length:71
Mass (Da):8,417
Last modified:October 11, 2004 - v1
Checksum:iCE9FF2E890E78219
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY442175 Genomic DNA. Translation: AAS07631.1.
U00096 Genomic DNA. Translation: AAC74697.1.
AP009048 Genomic DNA. Translation: BAE76484.1.
PIRiC64919.
RefSeqiNP_416142.1. NC_000913.3.
WP_000217950.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74697; AAC74697; b1625.
BAE76484; BAE76484; BAE76484.
GeneIDi946152.
KEGGiecj:JW1617.
eco:b1625.
PATRICi32118552. VBIEscCol129921_1696.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY442175 Genomic DNA. Translation: AAS07631.1.
U00096 Genomic DNA. Translation: AAC74697.1.
AP009048 Genomic DNA. Translation: BAE76484.1.
PIRiC64919.
RefSeqiNP_416142.1. NC_000913.3.
WP_000217950.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JQTNMR-A1-71[»]
ProteinModelPortaliP64467.
SMRiP64467.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259630. 18 interactors.
DIPiDIP-48011N.
IntActiP64467. 3 interactors.
MINTiMINT-8175315.
STRINGi511145.b1625.

Proteomic databases

PaxDbiP64467.
PRIDEiP64467.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74697; AAC74697; b1625.
BAE76484; BAE76484; BAE76484.
GeneIDi946152.
KEGGiecj:JW1617.
eco:b1625.
PATRICi32118552. VBIEscCol129921_1696.

Organism-specific databases

EchoBASEiEB4050.
EcoGeneiEG14303. cnu.

Phylogenomic databases

eggNOGiENOG4105QZ5. Bacteria.
ENOG4111UG9. LUCA.
HOGENOMiHOG000219358.
OMAiPKSVWHF.

Enzyme and pathway databases

BioCyciEcoCyc:G6869-MONOMER.
ECOL316407:JW1617-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP64467.
PROiP64467.

Family and domain databases

Gene3Di1.20.1280.40. 1 hit.
InterProiIPR007985. Hemolysn_expr_modulating_HHA.
[Graphical view]
PfamiPF05321. HHA. 1 hit.
[Graphical view]
SUPFAMiSSF68989. SSF68989. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCNU_ECOLI
AccessioniPrimary (citable) accession number: P64467
Secondary accession number(s): P76179, Q2MB72, Q5VJ24
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: November 2, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Expression becomes maximal as cells enter the stationary phase but decreases later in the stationary phase.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.