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Protein

OriC-binding nucleoid-associated protein

Gene

cnu

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Modifies the set of genes regulated by H-NS; Hha and Cnu (YdgT) increase the number of genes bound by H-NS/StpA and may also modulate the oligomerization of the H-NS/StpA-complex on DNA (PubMed:23543115). The complex formed with H-NS binds to the specific 26-bp cnb site in the origin of replication oriC (PubMed:16199570). Can complement, at least partially, the absence of the Hha protein in hha mutants.2 Publications

Miscellaneous

Expression becomes maximal as cells enter the stationary phase but decreases later in the stationary phase.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei44Interacts with H-NS1

GO - Molecular functioni

GO - Biological processi

  • negative regulation of single-species biofilm formation on inanimate substrate Source: EcoCyc
  • positive regulation of DNA-templated transcription, termination Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:G6869-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
OriC-binding nucleoid-associated protein
Alternative name(s):
H-NS/StpA-binding protein 2
Transcription modulator YdgT
Gene namesi
Name:cnu1 Publication
Synonyms:ydgT
Ordered Locus Names:b1625, JW1617
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG14303. cnu.

Pathology & Biotechi

Disruption phenotypei

At 0.3 M NaCl up-regulation of 1 gene and down-regulation of 6 genes was observed; a double cnu-hha deletion up-regulated 134 and down-regulated 5 genes, most of which are thought to have been acquired horizontally and are also up-regulated in double hns-stpA deletions (PubMed:23543115).1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi44D → N: Abolishes heterocomplex formation with H-NS. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002017331 – 71OriC-binding nucleoid-associated proteinAdd BLAST71

Proteomic databases

PaxDbiP64467.
PRIDEiP64467.

Interactioni

Subunit structurei

Forms complexes with both H-NS and StpA.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
hnsP0ACF82EBI-551907,EBI-544934

Protein-protein interaction databases

BioGridi4259630. 18 interactors.
DIPiDIP-48011N.
IntActiP64467. 3 interactors.
MINTiMINT-8175315.
STRINGi511145.b1625.

Structurei

Secondary structure

171
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 12Combined sources6
Helixi17 – 29Combined sources13
Helixi33 – 51Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JQTNMR-A1-71[»]
ProteinModelPortaliP64467.
SMRiP64467.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP64467.

Family & Domainsi

Sequence similaritiesi

Belongs to the Hha/YmoA/Cnu family.Curated

Phylogenomic databases

eggNOGiENOG4105QZ5. Bacteria.
ENOG4111UG9. LUCA.
HOGENOMiHOG000219358.
OMAiPKSVWHF.

Family and domain databases

Gene3Di1.20.1280.40. 1 hit.
InterProiView protein in InterPro
IPR007985. Hemolysn_expr_modulating_HHA.
PfamiView protein in Pfam
PF05321. HHA. 1 hit.
SUPFAMiSSF68989. SSF68989. 1 hit.

Sequencei

Sequence statusi: Complete.

P64467-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVQDYLLKF RKISSLESLE KLYDHLNYTL TDDQELINMY RAADHRRAEL
60 70
VSGGRLFDLG QVPKSVWHYV Q
Length:71
Mass (Da):8,417
Last modified:October 11, 2004 - v1
Checksum:iCE9FF2E890E78219
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY442175 Genomic DNA. Translation: AAS07631.1.
U00096 Genomic DNA. Translation: AAC74697.1.
AP009048 Genomic DNA. Translation: BAE76484.1.
PIRiC64919.
RefSeqiNP_416142.1. NC_000913.3.
WP_000217950.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74697; AAC74697; b1625.
BAE76484; BAE76484; BAE76484.
GeneIDi946152.
KEGGiecj:JW1617.
eco:b1625.
PATRICi32118552. VBIEscCol129921_1696.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY442175 Genomic DNA. Translation: AAS07631.1.
U00096 Genomic DNA. Translation: AAC74697.1.
AP009048 Genomic DNA. Translation: BAE76484.1.
PIRiC64919.
RefSeqiNP_416142.1. NC_000913.3.
WP_000217950.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JQTNMR-A1-71[»]
ProteinModelPortaliP64467.
SMRiP64467.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259630. 18 interactors.
DIPiDIP-48011N.
IntActiP64467. 3 interactors.
MINTiMINT-8175315.
STRINGi511145.b1625.

Proteomic databases

PaxDbiP64467.
PRIDEiP64467.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74697; AAC74697; b1625.
BAE76484; BAE76484; BAE76484.
GeneIDi946152.
KEGGiecj:JW1617.
eco:b1625.
PATRICi32118552. VBIEscCol129921_1696.

Organism-specific databases

EchoBASEiEB4050.
EcoGeneiEG14303. cnu.

Phylogenomic databases

eggNOGiENOG4105QZ5. Bacteria.
ENOG4111UG9. LUCA.
HOGENOMiHOG000219358.
OMAiPKSVWHF.

Enzyme and pathway databases

BioCyciEcoCyc:G6869-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP64467.
PROiPR:P64467.

Family and domain databases

Gene3Di1.20.1280.40. 1 hit.
InterProiView protein in InterPro
IPR007985. Hemolysn_expr_modulating_HHA.
PfamiView protein in Pfam
PF05321. HHA. 1 hit.
SUPFAMiSSF68989. SSF68989. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCNU_ECOLI
AccessioniPrimary (citable) accession number: P64467
Secondary accession number(s): P76179, Q2MB72, Q5VJ24
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: February 15, 2017
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.