P64461 (LSRG_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Autoinducer 2-degrading protein LsrG
Short name=AI-2-degrading protein LsrG

Gene names

Name:	lsrG
Synonyms:	yneC
Ordered Locus Names:	b1518, JW1511

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	96 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the degradation of phospho-AI-2, thereby terminating induction of the lsr operon and closing the AI-2 signaling cycle. Catalyzes the cleavage of phosphorylated 4,5-dihydroxy-2,3-pentanedione (P-DPD) to 2-phosphoglycolic acid (PG) and another three-carbon compound. Ref.5
Subcellular location	Cytoplasm Potential.
Induction	In the absence of AI-2, repressed by LsrR. Induced by AI-2, via release of the LsrR repressor. In the absence of glucose, induced by cAMP-CRP by direct binding to the upstream region of the lsr promoter. Ref.3 Ref.4
Sequence similarities	Belongs to the LsrG family.

Ontologies

Keywords
Cellular component	Cytoplasm
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	catalytic activity Inferred from direct assay Ref.5PubMed 21454635. Source: EcoCyc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
nadE	P18843	1	EBI-1124339,EBI-548960

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 96	96	Autoinducer 2-degrading protein LsrG		PRO_0000168947

Sequences

	Sequence		Length	Mass (Da)
	P64461 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 138099193F125EB8	FASTA	96	11,255
	10 20 30 40 50 60 MHVTLVEINV HEDKVDEFIE VFRQNHLGSV QEEGNLRFDV LQDPEVNSRF YIYEAYKDED 70 80 90 AVAFHKTTPH YKTCVAKLES LMTGPRKKRL FNGLMP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Regulation of uptake and processing of the quorum-sensing autoinducer AI-2 in Escherichia coli." Xavier K.B., Bassler B.L. J. Bacteriol. 187:238-248(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Cyclic AMP (cAMP) and cAMP receptor protein influence both synthesis and uptake of extracellular autoinducer 2 in Escherichia coli." Wang L., Hashimoto Y., Tsao C.-Y., Valdes J.J., Bentley W.E. J. Bacteriol. 187:2066-2076(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Phosphorylation and processing of the quorum-sensing molecule autoinducer-2 in enteric bacteria." Xavier K.B., Miller S.T., Lu W., Kim J.H., Rabinowitz J., Pelczer I., Semmelhack M.F., Bassler B.L. ACS Chem. Biol. 2:128-136(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN PHOSPHO-AI-2 DEGRADATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC74591.1. AP009048 Genomic DNA. Translation: BAE76458.1.
PIR	A64906.
RefSeq	NP_416035.1. NC_000913.2. YP_489781.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P64461.
SMR	P64461. Positions 1-96.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-47853N.
IntAct	P64461. 3 interactions.
STRING	511145.b1518.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74591; AAC74591; b1518. BAE76458; BAE76458; BAE76458.
GeneID	12931234. 946073.
KEGG	ecj:Y75_p1493. eco:b1518.
PATRIC	32118332. VBIEscCol129921_1586.
Organism-specific databases
EchoBASE	EB3572.
EcoGene	EG13811. lsrG.
Phylogenomic databases
eggNOG	COG1359.
HOGENOM	HOG000145619.
KO	K11530.
OMA	HYLACVD.
ProtClustDB	PRK10486.
Enzyme and pathway databases
BioCyc	EcoCyc:G6805-MONOMER. ECOL316407:JW1511-MONOMER. MetaCyc:G6805-MONOMER.
Gene expression databases
Genevestigator	P64461.
Family and domain databases
InterPro	IPR007138. Antibiotic_mOase. IPR011008. Dimeric_a/b-barrel. [Graphical view]
Pfam	PF03992. ABM. 1 hit. [Graphical view]
SUPFAM	SSF54909. Dimer_A_B_barrel. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LSRG_ECOLI

Accession

Primary (citable) accession number: P64461
Secondary accession number(s): P76144, Q2MB98

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program