Reviewed, UniProtKB/Swiss-Prot P64461 (LSRG_ECOLI)

Names and origin

Protein names

Recommended name:
    Autoinducer 2-degrading protein lsrG
      Short name=AI-2-degrading protein lsrG

Gene names

Name:	lsrG
Synonyms:	yneC
Ordered Locus Names:	b1518, JW1511

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	96 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the degradation of phospho-AI-2, thereby terminating induction of the lsr operon and closing the AI-2 signaling cycle. Catalyzes the cleavage of phosphorylated 4,5-dihydroxy-2,3-pentanedione (P-DPD) to 2-phosphoglycolic acid (PG) and another three-carbon compound.
Subcellular location	CytoplasmPotential.
Induction	In the absence of AI-2, repressed by lsrR. Induced by AI-2, via release of the lsrR repressor. In the absence of glucose, induced by cAMP-CRP by direct binding to the upstream region of the lsr promoter.
Sequence similarities	Belongs to the lsrG family.

Ontologies

Keywords
Cellular component	Cytoplasm
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	antibiotic biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
nadE	P18843	1	EBI-1124339,EBI-548960

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 96	96	Autoinducer 2-degrading protein lsrG		PRO_0000168947

Sequences

	Sequence		Length	Mass (Da)
	P64461-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 138099193F125EB8	FASTA	96	11,255
	10 20 30 40 50 60 MHVTLVEINV HEDKVDEFIE VFRQNHLGSV QEEGNLRFDV LQDPEVNSRF YIYEAYKDED 70 80 90 AVAFHKTTPH YKTCVAKLES LMTGPRKKRL FNGLMP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Regulation of uptake and processing of the quorum-sensing autoinducer AI-2 in Escherichia coli." Xavier K.B., Bassler B.L. J. Bacteriol. 187:238-248(2005) [PubMed: 15601708] [Abstract] Cited for: INDUCTION. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Cyclic AMP (cAMP) and cAMP receptor protein influence both synthesis and uptake of extracellular autoinducer 2 in Escherichia coli." Wang L., Hashimoto Y., Tsao C.-Y., Valdes J.J., Bentley W.E. J. Bacteriol. 187:2066-2076(2005) [PubMed: 15743955] [Abstract] Cited for: INDUCTION. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Phosphorylation and processing of the quorum-sensing molecule autoinducer-2 in enteric bacteria." Xavier K.B., Miller S.T., Lu W., Kim J.H., Rabinowitz J., Pelczer I., Semmelhack M.F., Bassler B.L. ACS Chem. Biol. 2:128-136(2007) [PubMed: 17274596] [Abstract] Cited for: FUNCTION IN PHOSPHO-AI-2 DEGRADATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00096 Genomic DNA. Translation: AAC74591.1. AP009048 Genomic DNA. Translation: BAE76458.1.
PIR	A64906.
RefSeq	AP_002139.1. NP_416035.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P64461.
Genome annotation databases
GeneID	946073.
GenomeReviews	Gene locus b1518 in contig U00096_GR. Gene locus JW1511 in contig AP009048_GR.
KEGG	ecj:JW1511. eco:b1518.
Organism-specific databases
EchoBASE	EB3572.
EcoGene	EG13811. lsrG.
CMR	Search...
Phylogenomic databases
HOGENOM	P64461.
Enzyme and pathway databases
BioCyc	EcoCyc:G6805-MON.
Family and domain databases
InterPro	IPR007138. Antibiotic_mOase. [Graphical view]
Pfam	PF03992. ABM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSRG_ECOLI

Accession

Primary (citable) accession number: P64461
Secondary accession number(s): P76144, Q2MB98

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	November 25, 2008
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)