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P64461

- LSRG_ECOLI

UniProt

P64461 - LSRG_ECOLI

Protein

Autoinducer 2-degrading protein LsrG

Gene

lsrG

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Involved in the degradation of phospho-AI-2, thereby terminating induction of the lsr operon and closing the AI-2 signaling cycle. Catalyzes the cleavage of phosphorylated 4,5-dihydroxy-2,3-pentanedione (P-DPD) to 2-phosphoglycolic acid (PG) and another three-carbon compound.1 Publication

    GO - Molecular functioni

    1. catalytic activity Source: EcoCyc

    GO - Biological processi

    1. metabolic process Source: GOC

    Enzyme and pathway databases

    BioCyciEcoCyc:G6805-MONOMER.
    ECOL316407:JW1511-MONOMER.
    MetaCyc:G6805-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Autoinducer 2-degrading protein LsrG
    Short name:
    AI-2-degrading protein LsrG
    Gene namesi
    Name:lsrG
    Synonyms:yneC
    Ordered Locus Names:b1518, JW1511
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG13811. lsrG.

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 9696Autoinducer 2-degrading protein LsrGPRO_0000168947Add
    BLAST

    Expressioni

    Inductioni

    In the absence of AI-2, repressed by LsrR. Induced by AI-2, via release of the LsrR repressor. In the absence of glucose, induced by cAMP-CRP by direct binding to the upstream region of the lsr promoter.2 Publications

    Gene expression databases

    GenevestigatoriP64461.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    nadEP188431EBI-1124339,EBI-548960

    Protein-protein interaction databases

    DIPiDIP-47853N.
    IntActiP64461. 5 interactions.
    STRINGi511145.b1518.

    Structurei

    3D structure databases

    ProteinModelPortaliP64461.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 9190ABMAdd
    BLAST

    Sequence similaritiesi

    Belongs to the LsrG family.Curated
    Contains 1 ABM domain.Curated

    Phylogenomic databases

    eggNOGiCOG1359.
    HOGENOMiHOG000145619.
    KOiK11530.
    OMAiASILTRI.
    OrthoDBiEOG6K6VBX.

    Family and domain databases

    InterProiIPR007138. ABM-like.
    IPR011008. Dimeric_a/b-barrel.
    [Graphical view]
    PfamiPF03992. ABM. 1 hit.
    [Graphical view]
    SUPFAMiSSF54909. SSF54909. 1 hit.
    PROSITEiPS51725. ABM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P64461-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHVTLVEINV HEDKVDEFIE VFRQNHLGSV QEEGNLRFDV LQDPEVNSRF   50
    YIYEAYKDED AVAFHKTTPH YKTCVAKLES LMTGPRKKRL FNGLMP 96
    Length:96
    Mass (Da):11,255
    Last modified:October 11, 2004 - v1
    Checksum:i138099193F125EB8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC74591.1.
    AP009048 Genomic DNA. Translation: BAE76458.1.
    PIRiA64906.
    RefSeqiNP_416035.1. NC_000913.3.
    YP_489781.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74591; AAC74591; b1518.
    BAE76458; BAE76458; BAE76458.
    GeneIDi12931234.
    946073.
    KEGGiecj:Y75_p1493.
    eco:b1518.
    PATRICi32118332. VBIEscCol129921_1586.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC74591.1 .
    AP009048 Genomic DNA. Translation: BAE76458.1 .
    PIRi A64906.
    RefSeqi NP_416035.1. NC_000913.3.
    YP_489781.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P64461.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-47853N.
    IntActi P64461. 5 interactions.
    STRINGi 511145.b1518.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74591 ; AAC74591 ; b1518 .
    BAE76458 ; BAE76458 ; BAE76458 .
    GeneIDi 12931234.
    946073.
    KEGGi ecj:Y75_p1493.
    eco:b1518.
    PATRICi 32118332. VBIEscCol129921_1586.

    Organism-specific databases

    EchoBASEi EB3572.
    EcoGenei EG13811. lsrG.

    Phylogenomic databases

    eggNOGi COG1359.
    HOGENOMi HOG000145619.
    KOi K11530.
    OMAi ASILTRI.
    OrthoDBi EOG6K6VBX.

    Enzyme and pathway databases

    BioCyci EcoCyc:G6805-MONOMER.
    ECOL316407:JW1511-MONOMER.
    MetaCyc:G6805-MONOMER.

    Miscellaneous databases

    PROi P64461.

    Gene expression databases

    Genevestigatori P64461.

    Family and domain databases

    InterProi IPR007138. ABM-like.
    IPR011008. Dimeric_a/b-barrel.
    [Graphical view ]
    Pfami PF03992. ABM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54909. SSF54909. 1 hit.
    PROSITEi PS51725. ABM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. "Regulation of uptake and processing of the quorum-sensing autoinducer AI-2 in Escherichia coli."
      Xavier K.B., Bassler B.L.
      J. Bacteriol. 187:238-248(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Cyclic AMP (cAMP) and cAMP receptor protein influence both synthesis and uptake of extracellular autoinducer 2 in Escherichia coli."
      Wang L., Hashimoto Y., Tsao C.-Y., Valdes J.J., Bentley W.E.
      J. Bacteriol. 187:2066-2076(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Phosphorylation and processing of the quorum-sensing molecule autoinducer-2 in enteric bacteria."
      Xavier K.B., Miller S.T., Lu W., Kim J.H., Rabinowitz J., Pelczer I., Semmelhack M.F., Bassler B.L.
      ACS Chem. Biol. 2:128-136(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN PHOSPHO-AI-2 DEGRADATION.

    Entry informationi

    Entry nameiLSRG_ECOLI
    AccessioniPrimary (citable) accession number: P64461
    Secondary accession number(s): P76144, Q2MB98
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3