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Protein

(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase

Gene

lsrG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the degradation of phospho-AI-2, thereby terminating induction of the lsr operon and closing the AI-2 signaling cycle. Catalyzes the conversion of (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione (P-DPD) to 3-hydroxy-5-phosphonooxypentane-2,4-dione (P-HPD).UniRule annotation2 Publications

Catalytic activityi

(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione = 3-hydroxy-5-phosphonooxypentane-2,4-dione.UniRule annotation1 Publication

GO - Molecular functioni

  • (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase activity Source: EcoCyc
  • isomerase activity Source: EcoCyc
  • oxidoreductase activity Source: GO_Central

Keywordsi

Molecular functionIsomerase

Enzyme and pathway databases

BioCyciEcoCyc:G6805-MONOMER
MetaCyc:G6805-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomeraseUniRule annotation (EC:5.3.1.32UniRule annotation1 Publication)
Alternative name(s):
Autoinducer 2-degrading protein LsrGUniRule annotation
Short name:
AI-2-degrading protein LsrGUniRule annotation
Phospho-(S)-4,5-dihydroxy-2,3-pentanedione isomerase1 PublicationUniRule annotation
Phospho-AI-2 isomeraseUniRule annotation
Gene namesi
Name:lsrGUniRule annotation
Synonyms:yneC
Ordered Locus Names:b1518, JW1511
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13811 lsrG

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi25N → A: 3-fold lower specific activity. 1 Publication1
Mutagenesisi54E → A: No activity. 1 Publication1
Mutagenesisi65H → A: No activity. 1 Publication1
Mutagenesisi70H → A: 100-fold lower specific activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001689471 – 96(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomeraseAdd BLAST96

Proteomic databases

PaxDbiP64461
PRIDEiP64461

Expressioni

Inductioni

In the absence of AI-2, repressed by LsrR. Induced by AI-2, via release of the LsrR repressor. In the absence of glucose, induced by cAMP-CRP by direct binding to the upstream region of the lsr promoter.2 Publications

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi4260228, 12 interactors
DIPiDIP-47853N
IntActiP64461, 6 interactors
STRINGi316385.ECDH10B_1649

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QMQX-ray1.80A/B/C/D1-96[»]
ProteinModelPortaliP64461
SMRiP64461
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 91ABMUniRule annotationAdd BLAST90

Sequence similaritiesi

Belongs to the LsrG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105K34 Bacteria
COG1359 LUCA
HOGENOMiHOG000145619
InParanoidiP64461
KOiK11530
OMAiWRDTVAP

Family and domain databases

HAMAPiMF_02051 LsrG, 1 hit
InterProiView protein in InterPro
IPR007138 ABM_dom
IPR011008 Dimeric_a/b-barrel
IPR033672 LsrG
PfamiView protein in Pfam
PF03992 ABM, 1 hit
SUPFAMiSSF54909 SSF54909, 1 hit
PROSITEiView protein in PROSITE
PS51725 ABM, 1 hit

Sequencei

Sequence statusi: Complete.

P64461-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHVTLVEINV HEDKVDEFIE VFRQNHLGSV QEEGNLRFDV LQDPEVNSRF
60 70 80 90
YIYEAYKDED AVAFHKTTPH YKTCVAKLES LMTGPRKKRL FNGLMP
Length:96
Mass (Da):11,255
Last modified:October 11, 2004 - v1
Checksum:i138099193F125EB8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74591.1
AP009048 Genomic DNA Translation: BAE76458.1
PIRiA64906
RefSeqiNP_416035.1, NC_000913.3
WP_000558527.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74591; AAC74591; b1518
BAE76458; BAE76458; BAE76458
GeneIDi946073
KEGGiecj:JW1511
eco:b1518
PATRICifig|1411691.4.peg.749

Similar proteinsi

Entry informationi

Entry nameiLSRG_ECOLI
AccessioniPrimary (citable) accession number: P64461
Secondary accession number(s): P76144, Q2MB98
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: March 28, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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