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Protein

Orphan toxin OrtT

Gene

ortT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Acts as an orphan toxin which is important for maintaining cell fitness during stress related to the stringent response (decreased amino acid, purine and thymidine availability). Overexpression inhibits cell growth and increases the formation of persister cells. Causes 99.9% of cells to undergo bacterial lysis within 2 hours after induction; nucleoids condense, the cytoplasm seems empty and the periplasmic space enlarges. The intracellular ATP level decreases about 27-fold suggesting the membrane potential may be disrupted.1 Publication

GO - Biological processi

  • cytolysis Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Toxin

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciEcoCyc:G6756-MONOMER.
ECOL316407:JW5232-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Orphan toxin OrtT1 Publication
Gene namesi
Name:ortT1 Publication
Synonyms:ydcX
Ordered Locus Names:b1445, JW5232
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13767. ydcX.

Subcellular locationi

  • Cell inner membrane 1 Publication; Multi-pass membrane protein Curated

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei6 – 2621HelicalSequence analysisAdd
BLAST
Transmembranei34 – 5421HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Strongly represses cell swarming but no effect on cell swimming (PubMed:17122336). Increased metabolic activity in the presence of TMP, an inhibitor of dihydrofolate reductase (folA) and sulfamethoxazole, an inhibitor of dihydropteroate synthetase (folC). Cells grow faster than wild-type in the presence of TMP.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1 – 11M → T: Not toxic as no protein is produced. 1 Publication
Mutagenesisi38 – 381F → R: Reduced toxicity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5757Orphan toxin OrtTPRO_0000168933Add
BLAST

Proteomic databases

PaxDbiP64453.

Expressioni

Inductioni

By conditions that induce the stringent response such as increased expression of RelA, heat shock at 43 degres Celsius, probably under control of sigma factor E and/or H (rpoE, rpoH), or growth in the presence of trimethoprim (TMP), mupirocin or serine hydroxamate.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4262895. 100 interactions.
STRINGi511145.b1445.

Structurei

3D structure databases

ProteinModelPortaliP64453.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GhoT/OrtT toxin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410654E. Bacteria.
ENOG41129WE. LUCA.
HOGENOMiHOG000219287.
OMAiMSLYQKM.
OrthoDBiEOG6DG2Z7.

Family and domain databases

InterProiIPR019689. Toxin_GhoT/OrtT.
[Graphical view]
PfamiPF10753. DUF2566. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P64453-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLYQHMLVF YAVMAAIAFL ITWFLSHDKK RIRFLSAFLV GATWPMSFPV

ALLFSLF
Length:57
Mass (Da):6,646
Last modified:December 4, 2007 - v2
Checksum:i11D1EE35781F61B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74527.2.
AP009048 Genomic DNA. Translation: BAE76441.1.
PIRiH64896.
RefSeqiNP_415962.2. NC_000913.3.
WP_000061178.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74527; AAC74527; b1445.
BAE76441; BAE76441; BAE76441.
GeneIDi945936.
KEGGiecj:JW5232.
eco:b1445.
PATRICi32118182. VBIEscCol129921_1511.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74527.2.
AP009048 Genomic DNA. Translation: BAE76441.1.
PIRiH64896.
RefSeqiNP_415962.2. NC_000913.3.
WP_000061178.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP64453.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262895. 100 interactions.
STRINGi511145.b1445.

Proteomic databases

PaxDbiP64453.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74527; AAC74527; b1445.
BAE76441; BAE76441; BAE76441.
GeneIDi945936.
KEGGiecj:JW5232.
eco:b1445.
PATRICi32118182. VBIEscCol129921_1511.

Organism-specific databases

EchoBASEiEB3530.
EcoGeneiEG13767. ydcX.

Phylogenomic databases

eggNOGiENOG410654E. Bacteria.
ENOG41129WE. LUCA.
HOGENOMiHOG000219287.
OMAiMSLYQKM.
OrthoDBiEOG6DG2Z7.

Enzyme and pathway databases

BioCyciEcoCyc:G6756-MONOMER.
ECOL316407:JW5232-MONOMER.

Miscellaneous databases

PROiP64453.

Family and domain databases

InterProiIPR019689. Toxin_GhoT/OrtT.
[Graphical view]
PfamiPF10753. DUF2566. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Genome-wide screening of genes required for swarming motility in Escherichia coli K-12."
    Inoue T., Shingaki R., Hirose S., Waki K., Mori H., Fukui K.
    J. Bacteriol. 189:950-957(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
    Strain: K12 / BW25113.
  4. "Orphan toxin OrtT (YdcX) of Escherichia coli reduces growth during the stringent response."
    Islam S., Benedik M.J., Wood T.K.
    Toxins 7:299-321(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, LACK OF ANTITOXIN, SUBCELLULAR LOCATION, INDUCTION BY STRESS, DISRUPTION PHENOTYPE, MUTAGENESIS OF MET-1 AND PHE-38.
    Strain: K12 / BW25113.

Entry informationi

Entry nameiORTT_ECOLI
AccessioniPrimary (citable) accession number: P64453
Secondary accession number(s): P76109, Q2MBB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: December 4, 2007
Last modified: April 13, 2016
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Although this protein has high homology to GhoT, the toxic component of a type IV toxin-antitoxin module, no antitoxin has been found. GhoS (the antitoxin for GhoT), YdcY (the neighboring gene), 5'- and 3'-UTRs as well as approximately 620 bp lengths of the BW25113 genome DNA were tested.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.