P64306 (NTPA_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

BR0175, BS1330_I0175

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 220	220	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_0000178142
Regions
	Region	13 – 18	6	Substrate binding By similarity
	Region	74 – 75	2	Substrate binding By similarity
Sites
	Metal binding	45	1	Magnesium or manganese By similarity
	Metal binding	74	1	Magnesium or manganese By similarity
	Binding site	166	1	Substrate By similarity
	Binding site	186	1	Substrate By similarity
	Binding site	200	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P64306 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 0055354117112336	FASTA	220	23,795
	10 20 30 40 50 60 MRMLEKGKLI VASHNAGKLR EFDGLIGPFG FEVSSVAALG LPEPDETGTT FEENAYIKAL 70 80 90 100 110 120 AAAKATGFPA LSDDSGLMVD ALDGEPGVYT ANWAETEDGK RDFDMAMQKV ENLLQEKGAT 130 140 150 160 170 180 TPDKRKARFV SVICLAWPDG EAEYFRGEVE GTLVWPPRGN IGFGYDPVFL PDGYGKTFGE 190 200 210 220 MTAEEKHGWK PGDASALSHR ARAFKLFAEK ALNVVSAPAE

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed: 22038969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN29128.1. CP002997 Genomic DNA. Translation: AEM17540.1.
RefSeq	NP_697213.1. NC_004310.3.
3D structure databases
ProteinModelPortal	P64306.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1165832.
GenomeReviews	Gene locus BR0175 in contig AE014291_GR.
KEGG	bms:BR0175.
PATRIC	17788620. VBIBruSui107850_0183.
TIGR	BR0175.
Phylogenomic databases
HOGENOM	HBG697237.
OMA	GVYTADW.
PhylomeDB	P64306.
ProtClustDB	PRK00120.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_0175-MONOMER.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_BRUSU

Accession

Primary (citable) accession number: P64306
Secondary accession number(s): G0KBE8, Q8YEV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program