Skip Header

Contribute Send feedback
Read comments (?) or add your own

P64306 (NTPA_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Non-canonical purine NTP pyrophosphatase

EC=3.6.1.19
Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase
Gene names
Ordered Locus Names:BR0175, BS1330_I0175
OrganismBrucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]
Taxonomic identifier204722 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length220 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405

Catalytic activity

A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405

Cofactor

Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.

Subunit structure

Homodimer By similarity. HAMAP MF_01405

Sequence similarities

Belongs to the HAM1 NTPase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 220220Non-canonical purine NTP pyrophosphatase HAMAP MF_01405
PRO_0000178142

Regions

Region13 – 186Substrate binding By similarity
Region74 – 752Substrate binding By similarity

Sites

Metal binding451Magnesium or manganese By similarity
Metal binding741Magnesium or manganese By similarity
Binding site1661Substrate By similarity
Binding site1861Substrate By similarity
Binding site2001Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P64306 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 0055354117112336

FASTA22023,795
        10         20         30         40         50         60 
MRMLEKGKLI VASHNAGKLR EFDGLIGPFG FEVSSVAALG LPEPDETGTT FEENAYIKAL 

        70         80         90        100        110        120 
AAAKATGFPA LSDDSGLMVD ALDGEPGVYT ANWAETEDGK RDFDMAMQKV ENLLQEKGAT 

       130        140        150        160        170        180 
TPDKRKARFV SVICLAWPDG EAEYFRGEVE GTLVWPPRGN IGFGYDPVFL PDGYGKTFGE 

       190        200        210        220 
MTAEEKHGWK PGDASALSHR ARAFKLFAEK ALNVVSAPAE 

« Hide

References

[1]"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts."
Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. expand/collapse author list , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.
Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.
[2]"Revised genome sequence of Brucella suis 1330."
Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.
J. Bacteriol. 193:6410-6410(2011) [PubMed: 22038969] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014291 Genomic DNA. Translation: AAN29128.1.
CP002997 Genomic DNA. Translation: AEM17540.1.
RefSeqNP_697213.1. NC_004310.3.

3D structure databases

ProteinModelPortalP64306.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1165832.
GenomeReviewsGene locus BR0175 in contig AE014291_GR.
KEGGbms:BR0175.
PATRIC17788620. VBIBruSui107850_0183.
TIGRBR0175.

Phylogenomic databases

HOGENOMHBG697237.
OMAGVYTADW.
PhylomeDBP64306.
ProtClustDBPRK00120.

Enzyme and pathway databases

BioCycBSUI204722:BR_0175-MONOMER.

Family and domain databases

HAMAPMF_01405. Non_canon_purine_NTPase.
[Tree]
InterProIPR002637. Ham1p-like.
IPR020922. Nucleoside-triphosphatase.
[Graphical view]
KOK02428.
PANTHERPTHR11067. Ham1p_like. 1 hit.
PfamPF01725. Ham1p_like. 1 hit.
[Graphical view]
TIGRFAMsTIGR00042. TIGR00042. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNTPA_BRUSU
AccessionPrimary (citable) accession number: P64306
Secondary accession number(s): G0KBE8, Q8YEV5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Brucella suis

Brucella suis (strain 1330): entries and gene names

SIMILARITY comments

Index of protein domains and families