P64275 (GREA_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription elongation factor GreA

Alternative name(s):
Transcript cleavage factor GreA

Gene names

Name:	greA
Ordered Locus Names:	HP_0866

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	164 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function

Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides By similarity. HAMAP-Rule MF_00105

Sequence similarities

Belongs to the GreA/GreB family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of DNA-dependent transcription, elongation Inferred from electronic annotation. Source: HAMAP transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 164	164	Transcription elongation factor GreA HAMAP-Rule MF_00105		PRO_0000176931

Sequences

	Sequence		Length	Mass (Da)
	P64275 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 439E43A5FD0965BE	FASTA	164	18,199
	10 20 30 40 50 60 MNKEPMSMHG YNKICAELKQ LKEVERPNIV KEIDIARGHG DLKENAEYHA AKEKQRFIEA 70 80 90 100 110 120 RIVDLSEIVA NAQVIDPSAL AHNKVSFGST IKILNLDNDK EFSYTIVGSV ESDPAKGLIS 130 140 150 160 FGSPIAKSLI GKSKGDAVSI QLPNGESDFE ILDIYYKEIC FDEN

References

[1]

"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000511 Genomic DNA. Translation: AAD07908.1.
PIR	B64628.
RefSeq	NP_207660.1. NC_000915.1. YP_006934780.1. NC_018939.1.
3D structure databases
ProteinModelPortal	P64275.
ModBase	Search...
Protein-protein interaction databases
STRING	85962.HP0866.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD07908; AAD07908; HP_0866.
GeneID	13870050. 899395.
KEGG	heo:C694_04435. hpy:HP0866.
PATRIC	20593021. VBIHelPyl33062_0898.
Phylogenomic databases
eggNOG	COG0782.
KO	K03624.
OMA	HNEGRIA.
ProtClustDB	PRK00226.
Family and domain databases
Gene3D	1.10.287.180. 1 hit. 3.10.50.30. 1 hit.
HAMAP	MF_00105. GreA_GreB.
InterPro	IPR018151. TF_GreA/GreB_CS. IPR006359. Tscrpt_elong_fac_GreA. IPR023459. Tscrpt_elong_fac_GreA/B. IPR001437. Tscrpt_elong_fac_GreA/B_C. IPR022691. Tscrpt_elong_fac_GreA/B_N. [Graphical view]
Pfam	PF01272. GreA_GreB. 1 hit. PF03449. GreA_GreB_N. 1 hit. [Graphical view]
PIRSF	PIRSF006092. GreA_GreB. 1 hit.
SUPFAM	SSF46557. GreA_GreB. 1 hit.
TIGRFAMs	TIGR01462. greA. 1 hit.
PROSITE	PS00829. GREAB_1. 1 hit. PS00830. GREAB_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GREA_HELPY

Accession

Primary (citable) accession number: P64275
Secondary accession number(s): P55978

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program