P64271 (GREA_BRUME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription elongation factor GreA

Alternative name(s):
Transcript cleavage factor GreA

Gene names

Name:	greA
Ordered Locus Names:	BMEI0508

Organism

Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]

Taxonomic identifier

224914 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella melitensis › Brucella melitensis bv. 1

Protein attributes

Sequence length	157 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function

Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides By similarity. HAMAP-Rule MF_00105

Sequence similarities

Belongs to the GreA/GreB family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	regulation of DNA-dependent transcription, elongation Inferred from electronic annotation. Source: HAMAP transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 157	157	Transcription elongation factor GreA HAMAP-Rule MF_00105		PRO_0000176910

Sequences

	Sequence		Length	Mass (Da)
	P64271 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 5651BAFED6942B3F	FASTA	157	17,529
	10 20 30 40 50 60 MEKFPMTPRG FEKLKEELRW RQQSERPRII EAIAEARAHG DLSENAEYHA AKEAQSLNEG 70 80 90 100 110 120 RINELEDLVA RAEVIDVSKL TGDRIKFGAT VTMIDEDTEE EKIYQIVGDQ EADVKEGRIS 130 140 150 ISSPIARALI GKGEGDTIEV NAPGGSRSYE IIALKFV

References

[1]

"The genome sequence of the facultative intracellular pathogen Brucella melitensis." DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T., Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G., Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E., Selkov E. , Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J., Haselkorn R., Kyrpides N.C., Overbeek R. Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 16M / ATCC 23456 / NCTC 10094.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008917 Genomic DNA. Translation: AAL51689.1.
PIR	AF3315.
RefSeq	NP_539425.1. NC_003317.1.
3D structure databases
ProteinModelPortal	P64271.
SMR	P64271. Positions 2-157.
ModBase	Search...
Protein-protein interaction databases
STRING	224914.BMEI0508.
Proteomic databases
PRIDE	P64271.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL51689; AAL51689; BMEI0508.
GeneID	1196219.
KEGG	bme:BMEI0508.
PATRIC	17801119. VBIBruMel146950_2885.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000241145.
KO	K03624.
OMA	HNEGRIA.
ProtClustDB	PRK00226.
Family and domain databases
Gene3D	1.10.287.180. 1 hit. 3.10.50.30. 1 hit.
HAMAP	MF_00105. GreA_GreB.
InterPro	IPR018151. TF_GreA/GreB_CS. IPR006359. Tscrpt_elong_fac_GreA. IPR023459. Tscrpt_elong_fac_GreA/B. IPR001437. Tscrpt_elong_fac_GreA/B_C. IPR022691. Tscrpt_elong_fac_GreA/B_N. [Graphical view]
Pfam	PF01272. GreA_GreB. 1 hit. PF03449. GreA_GreB_N. 1 hit. [Graphical view]
PIRSF	PIRSF006092. GreA_GreB. 1 hit.
SUPFAM	SSF46557. GreA_GreB. 1 hit.
TIGRFAMs	TIGR01462. greA. 1 hit.
PROSITE	PS00829. GREAB_1. 1 hit. PS00830. GREAB_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GREA_BRUME

Accession

Primary (citable) accession number: P64271
Secondary accession number(s): Q8YID6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program