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Reviewed, UniProtKB/Swiss-Prot P64248 (ASNH_MYCBO)

Last modified November 3, 2009. Version 32. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Putative asparagine synthetase [glutamine-hydrolyzing]
    EC=6.3.5.4
Gene names
Name: asnB
Ordered Locus Names: Mb2224
OrganismMycobacterium bovis [Complete proteome] [HAMAP]
Taxonomic identifier1765 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

Protein attributes

Sequence length652 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Catalytic activity

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathway

Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.

Induction

Induced in response to the thiol oxidant diamide. Ref.2

Sequence similarities

Belongs to the asparagine synthetase family.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 652652Putative asparagine synthetase [glutamine-hydrolyzing]
PRO_0000056936

Regions

Domain2 – 231230Glutamine amidotransferase type-2

Sites

Active site21For GATase activity By similarity

Sequences

Sequence LengthMass (Da)Tools
P64248-1 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: C4624495A845F790

FASTA65272,150
        10         20         30         40         50         60 
MCGLLAFVAA PAGAAGPEGA DAASAIARAS HLMRHRGPDE SGTWHAVDGA SGGVVFGFNR 

        70         80         90        100        110        120 
LSIIDIAHSH QPLRWGPPEA PDRYVLVFNG EIYNYLELRD ELRTQHGAVF ATDGDGEAIL 

       130        140        150        160        170        180 
AGYHHWGTEV LQRLRGMFAF ALWDTVTREL FCARDPFGIK PLFIATGAGG TAVASEKKCL 

       190        200        210        220        230        240 
LDLVELVGFD TEIDHRALQH YTVLQYVPEP ETLHRGVRRL ESGCFARIRA DQLAPVITRY 

       250        260        270        280        290        300 
FVPRFAASPI TNDNDQARYD EITAVLEDSV AKHMRADVTV GAFLSGGIDS TAIAALAIRH 

       310        320        330        340        350        360 
NPRLITFTTG FEREGFSEID VAVASAEAIG ARHIAKVVSA DEFVAALPEI VWYLDEPVAD 

       370        380        390        400        410        420 
PALVPLFFVA REARKHVKVV LSGEGADELF GGYTIYREPL SLRPFDYLPK PLRRSMGKVS 

       430        440        450        460        470        480 
KPLPEGMRGK SLLHRGSLTL EERYYGNARS FSGAQLREVL PGFRPDWTHT DVTAPVYAES 

       490        500        510        520        530        540 
AGWDPVARMQ HIDLFTWLRG DILVKADKIT MANSLELRVP FLDPEVFAVA SRLPAGAKIT 

       550        560        570        580        590        600 
RTTTKYALRR ALEPIVPAHV LHRPKLGFPV PIRHWLRAGE LLEWAYATVG SSQAGHLVDI 

       610        620        630        640        650 
AAVYRMLDEH RCGSSDHSRR LWTMLIFMLW HAIFVEHSVV PQISEPQYPV QL 

« Hide

Cross-references

Sequence databases

BX248341 Genomic DNA. Translation: CAD97077.1.
RefSeqNP_855873.1.

3D structure databases

HSSPHSSP built from PDB template 1CT9 based on UniProtKB P22106.
ModBaseSearch...

Genome annotation databases

GeneID1091346.
GenomeReviewsGene locus Mb2224 in contig BX248333_GR.
KEGGmbo:Mb2224.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMP64248.
OMAKPFDYLP.

Enzyme and pathway databases

BRENDA6.3.5.4. 3091.

Family and domain databases

InterProIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR000583. GATase_2.
IPR017932. GATase_II.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
Gene3DG3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PfamPF00733. Asn_synthase. 1 hit.
PF00310. GATase_2. 1 hit.
[Graphical view]
TIGRFAMsTIGR01536. asn_synth_AEB. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASNH_MYCBO
AccessionPrimary (citable) accession number: P64248
Secondary accession number(s): Q10374
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: November 3, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents