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P64248

- ASNH_MYCBO

UniProt

P64248 - ASNH_MYCBO

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Protein
Putative asparagine synthetase [glutamine-hydrolyzing]
Gene
asnB, Mb2224
Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activity By similarity
Binding sitei115 – 1151Glutamine By similarity
Sitei384 – 3841Important for beta-aspartyl-AMP intermediate formation By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi382 – 3832ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. asparagine synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC

GO - Biological processi

  1. L-asparagine biosynthetic process Source: UniProtKB-UniPathway
  2. glutamine metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative asparagine synthetase [glutamine-hydrolyzing] (EC:6.3.5.4)
Gene namesi
Name:asnB
Ordered Locus Names:Mb2224
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001419: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 652651Putative asparagine synthetase [glutamine-hydrolyzing]
PRO_0000056936Add
BLAST

Expressioni

Inductioni

Induced in response to the thiol oxidant diamide.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi233413.Mb2224.

Structurei

3D structure databases

ProteinModelPortaliP64248.
SMRiP64248. Positions 30-398.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 231230Glutamine amidotransferase type-2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni60 – 645Glutamine binding By similarity
Regioni89 – 913Glutamine binding By similarity

Sequence similaritiesi

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0367.
HOGENOMiHOG000027495.
KOiK01953.
OMAiAMRHNPN.
OrthoDBiEOG6P8TM1.

Family and domain databases

Gene3Di3.40.50.620. 2 hits.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P64248-1 [UniParc]FASTAAdd to Basket

« Hide

MCGLLAFVAA PAGAAGPEGA DAASAIARAS HLMRHRGPDE SGTWHAVDGA    50
SGGVVFGFNR LSIIDIAHSH QPLRWGPPEA PDRYVLVFNG EIYNYLELRD 100
ELRTQHGAVF ATDGDGEAIL AGYHHWGTEV LQRLRGMFAF ALWDTVTREL 150
FCARDPFGIK PLFIATGAGG TAVASEKKCL LDLVELVGFD TEIDHRALQH 200
YTVLQYVPEP ETLHRGVRRL ESGCFARIRA DQLAPVITRY FVPRFAASPI 250
TNDNDQARYD EITAVLEDSV AKHMRADVTV GAFLSGGIDS TAIAALAIRH 300
NPRLITFTTG FEREGFSEID VAVASAEAIG ARHIAKVVSA DEFVAALPEI 350
VWYLDEPVAD PALVPLFFVA REARKHVKVV LSGEGADELF GGYTIYREPL 400
SLRPFDYLPK PLRRSMGKVS KPLPEGMRGK SLLHRGSLTL EERYYGNARS 450
FSGAQLREVL PGFRPDWTHT DVTAPVYAES AGWDPVARMQ HIDLFTWLRG 500
DILVKADKIT MANSLELRVP FLDPEVFAVA SRLPAGAKIT RTTTKYALRR 550
ALEPIVPAHV LHRPKLGFPV PIRHWLRAGE LLEWAYATVG SSQAGHLVDI 600
AAVYRMLDEH RCGSSDHSRR LWTMLIFMLW HAIFVEHSVV PQISEPQYPV 650
QL 652
Length:652
Mass (Da):72,150
Last modified:October 11, 2004 - v1
Checksum:iC4624495A845F790
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX248333 Genomic DNA. Translation: CDO43478.1.
RefSeqiNP_855873.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCAD97077; CAD97077; Mb2224.
GeneIDi1091346.
KEGGimbo:Mb2224.
PATRICi18006588. VBIMycBov88188_2440.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX248333 Genomic DNA. Translation: CDO43478.1 .
RefSeqi NP_855873.1. NC_002945.3.

3D structure databases

ProteinModelPortali P64248.
SMRi P64248. Positions 30-398.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 233413.Mb2224.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD97077 ; CAD97077 ; Mb2224 .
GeneIDi 1091346.
KEGGi mbo:Mb2224.
PATRICi 18006588. VBIMycBov88188_2440.

Phylogenomic databases

eggNOGi COG0367.
HOGENOMi HOG000027495.
KOi K01953.
OMAi AMRHNPN.
OrthoDBi EOG6P8TM1.

Enzyme and pathway databases

UniPathwayi UPA00134 ; UER00195 .

Family and domain databases

Gene3Di 3.40.50.620. 2 hits.
3.60.20.10. 1 hit.
InterProi IPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view ]
PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMi SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01536. asn_synth_AEB. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-935 / AF2122/97.
  2. "Thiol specific oxidative stress response in Mycobacteria."
    Dosanjh N.S., Rawat M., Chung J.-H., Av-Gay Y.
    FEMS Microbiol. Lett. 249:87-94(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INDUCTION.
    Strain: BCG / Pasteur.

Entry informationi

Entry nameiASNH_MYCBO
AccessioniPrimary (citable) accession number: P64248
Secondary accession number(s): Q10374, X2BKF1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: June 11, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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