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Protein

Putative asparagine synthetase [glutamine-hydrolyzing]

Gene

asnB

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathway: L-asparagine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. Putative asparagine synthetase [glutamine-hydrolyzing] (asnB)
This subpathway is part of the pathway L-asparagine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route), the pathway L-asparagine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activityBy similarity
Binding sitei115 – 1151GlutamineBy similarity
Sitei384 – 3841Important for beta-aspartyl-AMP intermediate formationBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi382 – 3832ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.

Protein family/group databases

MEROPSiC44.976.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative asparagine synthetase [glutamine-hydrolyzing] (EC:6.3.5.4)
Gene namesi
Name:asnB
Ordered Locus Names:Mb2224
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 652651Putative asparagine synthetase [glutamine-hydrolyzing]PRO_0000056936Add
BLAST

Expressioni

Inductioni

Induced in response to the thiol oxidant diamide.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP64248.
SMRiP64248. Positions 30-398.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 231230Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni60 – 645Glutamine bindingBy similarity
Regioni89 – 913Glutamine bindingBy similarity

Sequence similaritiesi

Belongs to the asparagine synthetase family.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0367.
HOGENOMiHOG000027495.
KOiK01953.
OMAiAMRHNPN.
OrthoDBiEOG6P8TM1.

Family and domain databases

Gene3Di3.40.50.620. 2 hits.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P64248-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGLLAFVAA PAGAAGPEGA DAASAIARAS HLMRHRGPDE SGTWHAVDGA
60 70 80 90 100
SGGVVFGFNR LSIIDIAHSH QPLRWGPPEA PDRYVLVFNG EIYNYLELRD
110 120 130 140 150
ELRTQHGAVF ATDGDGEAIL AGYHHWGTEV LQRLRGMFAF ALWDTVTREL
160 170 180 190 200
FCARDPFGIK PLFIATGAGG TAVASEKKCL LDLVELVGFD TEIDHRALQH
210 220 230 240 250
YTVLQYVPEP ETLHRGVRRL ESGCFARIRA DQLAPVITRY FVPRFAASPI
260 270 280 290 300
TNDNDQARYD EITAVLEDSV AKHMRADVTV GAFLSGGIDS TAIAALAIRH
310 320 330 340 350
NPRLITFTTG FEREGFSEID VAVASAEAIG ARHIAKVVSA DEFVAALPEI
360 370 380 390 400
VWYLDEPVAD PALVPLFFVA REARKHVKVV LSGEGADELF GGYTIYREPL
410 420 430 440 450
SLRPFDYLPK PLRRSMGKVS KPLPEGMRGK SLLHRGSLTL EERYYGNARS
460 470 480 490 500
FSGAQLREVL PGFRPDWTHT DVTAPVYAES AGWDPVARMQ HIDLFTWLRG
510 520 530 540 550
DILVKADKIT MANSLELRVP FLDPEVFAVA SRLPAGAKIT RTTTKYALRR
560 570 580 590 600
ALEPIVPAHV LHRPKLGFPV PIRHWLRAGE LLEWAYATVG SSQAGHLVDI
610 620 630 640 650
AAVYRMLDEH RCGSSDHSRR LWTMLIFMLW HAIFVEHSVV PQISEPQYPV

QL
Length:652
Mass (Da):72,150
Last modified:October 11, 2004 - v1
Checksum:iC4624495A845F790
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO43478.1.
RefSeqiNP_855873.1. NC_002945.3.
WP_003411413.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCDO43478; CDO43478; Mb2224.
GeneIDi1091346.
KEGGimbo:Mb2224.
PATRICi18006588. VBIMycBov88188_2440.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO43478.1.
RefSeqiNP_855873.1. NC_002945.3.
WP_003411413.1. NC_002945.3.

3D structure databases

ProteinModelPortaliP64248.
SMRiP64248. Positions 30-398.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC44.976.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCDO43478; CDO43478; Mb2224.
GeneIDi1091346.
KEGGimbo:Mb2224.
PATRICi18006588. VBIMycBov88188_2440.

Phylogenomic databases

eggNOGiCOG0367.
HOGENOMiHOG000027495.
KOiK01953.
OMAiAMRHNPN.
OrthoDBiEOG6P8TM1.

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.

Family and domain databases

Gene3Di3.40.50.620. 2 hits.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-935 / AF2122/97.
  2. "Thiol specific oxidative stress response in Mycobacteria."
    Dosanjh N.S., Rawat M., Chung J.-H., Av-Gay Y.
    FEMS Microbiol. Lett. 249:87-94(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INDUCTION.
    Strain: BCG / Pasteur.

Entry informationi

Entry nameiASNH_MYCBO
AccessioniPrimary (citable) accession number: P64248
Secondary accession number(s): Q10374, X2BKF1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: June 24, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.