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P64247 (ASNH_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative asparagine synthetase [glutamine-hydrolyzing]

EC=6.3.5.4
Gene names
Name:asnB
Ordered Locus Names:Rv2201, MT2257
ORF Names:MTCY190.12
OrganismMycobacterium tuberculosis [Reference proteome] [HAMAP]
Taxonomic identifier1773 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

Protein attributes

Sequence length652 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathway

Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.

Sequence similarities

Belongs to the asparagine synthetase family.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 652651Putative asparagine synthetase [glutamine-hydrolyzing]
PRO_0000056935

Regions

Domain2 – 231230Glutamine amidotransferase type-2
Nucleotide binding382 – 3832ATP By similarity
Region60 – 645Glutamine binding By similarity
Region89 – 913Glutamine binding By similarity

Sites

Active site21For GATase activity By similarity
Binding site1151Glutamine By similarity
Site3841Important for beta-aspartyl-AMP intermediate formation By similarity

Sequences

Sequence LengthMass (Da)Tools
P64247 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: C4624495A845F790

FASTA65272,150
        10         20         30         40         50         60 
MCGLLAFVAA PAGAAGPEGA DAASAIARAS HLMRHRGPDE SGTWHAVDGA SGGVVFGFNR 

        70         80         90        100        110        120 
LSIIDIAHSH QPLRWGPPEA PDRYVLVFNG EIYNYLELRD ELRTQHGAVF ATDGDGEAIL 

       130        140        150        160        170        180 
AGYHHWGTEV LQRLRGMFAF ALWDTVTREL FCARDPFGIK PLFIATGAGG TAVASEKKCL 

       190        200        210        220        230        240 
LDLVELVGFD TEIDHRALQH YTVLQYVPEP ETLHRGVRRL ESGCFARIRA DQLAPVITRY 

       250        260        270        280        290        300 
FVPRFAASPI TNDNDQARYD EITAVLEDSV AKHMRADVTV GAFLSGGIDS TAIAALAIRH 

       310        320        330        340        350        360 
NPRLITFTTG FEREGFSEID VAVASAEAIG ARHIAKVVSA DEFVAALPEI VWYLDEPVAD 

       370        380        390        400        410        420 
PALVPLFFVA REARKHVKVV LSGEGADELF GGYTIYREPL SLRPFDYLPK PLRRSMGKVS 

       430        440        450        460        470        480 
KPLPEGMRGK SLLHRGSLTL EERYYGNARS FSGAQLREVL PGFRPDWTHT DVTAPVYAES 

       490        500        510        520        530        540 
AGWDPVARMQ HIDLFTWLRG DILVKADKIT MANSLELRVP FLDPEVFAVA SRLPAGAKIT 

       550        560        570        580        590        600 
RTTTKYALRR ALEPIVPAHV LHRPKLGFPV PIRHWLRAGE LLEWAYATVG SSQAGHLVDI 

       610        620        630        640        650 
AAVYRMLDEH RCGSSDHSRR LWTMLIFMLW HAIFVEHSVV PQISEPQYPV QL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000516 Genomic DNA. Translation: AAK46543.1.
AL123456 Genomic DNA. Translation: CCP44978.1.
PIRB70785.
RefSeqNP_216717.1. NC_000962.3.
NP_336729.1. NC_002755.2.
YP_006515621.1. NC_018143.2.

3D structure databases

ProteinModelPortalP64247.
SMRP64247. Positions 2-590.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING83332.Rv2201.

Proteomic databases

PRIDEP64247.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK46543; AAK46543; MT2257.
CCP44978; CCP44978; Rv2201.
GeneID13318890.
888472.
924169.
KEGGmtc:MT2257.
mtu:Rv2201.
mtv:RVBD_2201.
PATRIC18126716. VBIMycTub22151_2470.

Organism-specific databases

TubercuListRv2201.

Phylogenomic databases

eggNOGCOG0367.
HOGENOMHOG000027495.
KOK01953.
OMAAFVIWDR.
OrthoDBEOG6P8TM1.
ProtClustDBCLSK2484247.

Enzyme and pathway databases

BioCycMTBCDC:MT2257-MONOMER.
MTBRV:RV2201-MONOMER.
UniPathwayUPA00134; UER00195.

Family and domain databases

Gene3D3.40.50.620. 2 hits.
InterProIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFPIRSF001589. Asn_synthetase_glu-h. 1 hit.
TIGRFAMsTIGR01536. asn_synth_AEB. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASNH_MYCTU
AccessionPrimary (citable) accession number: P64247
Secondary accession number(s): L0TAH2, Q10374
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: March 19, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh

Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names