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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi9 – 14FADUniRule annotation6

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Synonyms:gid
Ordered Locus Names:SAV1251
OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
Taxonomic identifieri158878 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000002481 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001172621 – 435Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOAdd BLAST435

Proteomic databases

PaxDbiP64234.
PRIDEiP64234.

2D gel databases

World-2DPAGE0002:P64234.

Interactioni

Protein-protein interaction databases

STRINGi158878.SAV1251.

Structurei

3D structure databases

ProteinModelPortaliP64234.
SMRiP64234.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
KOiK04094.
OMAiDYLNCPM.
PhylomeDBiP64234.

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P64234-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQTVNVIGA GLAGSEAAYQ LAERGIKVNL IEMRPVKQTP AHHTDKFAEL
60 70 80 90 100
VCSNSLRGNA LTNGVGVLKE EMRRLNSIII EAADKARVPA GGALAVDRHD
110 120 130 140 150
FSGYITETLK NHENITVINE EINAIPDGYT IIATGPLTTE TLAQEIVDIT
160 170 180 190 200
GKDQLYFYDA AAPIIEKESI DMDKVYLKSR YDKGEAAYLN CPMTEDEFNR
210 220 230 240 250
FYDAVLEAEV APVNSFEKEK YFEGCMPFEV MAERGRKTLL FGPMKPVGLE
260 270 280 290 300
DPKTGKRPYA VVQLRQDDAA GTLYNIVGFQ THLKWGAQKE VIKLIPGLEN
310 320 330 340 350
VDIVRYGVMH RNTFINSPDV LNEKYELISQ PNIQFAGQMT GVEGYVESAA
360 370 380 390 400
SGLVAGINLA HKILGKGEVV FPRETMIGSM AYYISHAKNN KNFQPMNANF
410 420 430
GLLPSLETRI KDKKERYEAQ ANRALDYLEN FKKTL
Length:435
Mass (Da):48,371
Last modified:October 11, 2004 - v1
Checksum:iB76C254394051425
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA. Translation: BAB57413.1.
RefSeqiWP_000195254.1. NC_002758.2.

Genome annotation databases

EnsemblBacteriaiBAB57413; BAB57413; SAV1251.
GeneIDi28381190.
KEGGisav:SAV1251.
PATRICi19563215. VBIStaAur52173_1285.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA. Translation: BAB57413.1.
RefSeqiWP_000195254.1. NC_002758.2.

3D structure databases

ProteinModelPortaliP64234.
SMRiP64234.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi158878.SAV1251.

2D gel databases

World-2DPAGE0002:P64234.

Proteomic databases

PaxDbiP64234.
PRIDEiP64234.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB57413; BAB57413; SAV1251.
GeneIDi28381190.
KEGGisav:SAV1251.
PATRICi19563215. VBIStaAur52173_1285.

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
KOiK04094.
OMAiDYLNCPM.
PhylomeDBiP64234.

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMFO_STAAM
AccessioniPrimary (citable) accession number: P64234
Secondary accession number(s): Q99UM0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: November 2, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.