P64207 (GCH1_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTP cyclohydrolase 1
EC=3.5.4.16

Alternative name(s):
GTP cyclohydrolase I
Short name=GTP-CH-I

Gene names

Name:	folE
Synonyms:	gchA
Ordered Locus Names:	Rv3609c, MT3713
ORF Names:	MTCY07H7B.13

Organism

Mycobacterium tuberculosis [Reference proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	GTP + H2O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate. HAMAP-Rule MF_00223
Pathway	Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1. HAMAP-Rule MF_00223
Subunit structure	Toroid-shaped homodecamer, composed of two pentamers of five dimers By similarity.
Sequence similarities	Belongs to the GTP cyclohydrolase I family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	GTP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	7,8-dihydroneopterin 3'-triphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway growth Inferred from mutant phenotype PubMed 12657046. Source: MTBBASE one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP tetrahydrofolate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP cyclohydrolase I activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 202	202	GTP cyclohydrolase 1 HAMAP-Rule MF_00223		PRO_0000119423
Sites
	Metal binding	90	1	Zinc By similarity
	Metal binding	93	1	Zinc By similarity
	Metal binding	163	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	P64207 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 7ED8293B83CB39DC	FASTA	202	22,395
	10 20 30 40 50 60 MSQLDSRSAS ARIRVFDQQR AEAAVRELLY AIGEDPDRDG LVATPSRVAR SYREMFAGLY 70 80 90 100 110 120 TDPDSVLNTM FDEDHDELVL VKEIPMYSTC EHHLVAFHGV AHVGYIPGDD GRVTGLSKIA 130 140 150 160 170 180 RLVDLYAKRP QVQERLTSQI ADALMKKLDP RGVIVVIEAE HLCMAMRGVR KPGSVTTTSA 190 200 VRGLFKTNAA SRAEALDLIL RK

References

[1]

"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.

[2]

"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842583 Genomic DNA. Translation: CAB08935.1. AE000516 Genomic DNA. Translation: AAK48072.1. AL123456 Genomic DNA. Translation: CCP46432.1.
PIR	B70956.
RefSeq	NP_218126.1. NC_000962.3. NP_338258.1. NC_002755.2. YP_006517098.1. NC_018143.1.
3D structure databases
ProteinModelPortal	P64207.
SMR	P64207. Positions 23-199.
ModBase	Search...
Protein-protein interaction databases
STRING	83332.Rv3609c.
Proteomic databases
PRIDE	P64207.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK48072; AAK48072; MT3713.
GeneID	13317217. 885346. 922777.
KEGG	mtc:MT3713. mtu:Rv3609c. mtv:RVBD_3609c.
PATRIC	18129919. VBIMycTub22151_4054.
Organism-specific databases
TubercuList	Rv3609c.
Phylogenomic databases
eggNOG	COG0302.
HOGENOM	HOG000221222.
KO	K01495.
OMA	LIRHQPA.
ProtClustDB	PRK09347.
Enzyme and pathway databases
UniPathway	UPA00848; UER00151.
Family and domain databases
HAMAP	MF_00223. FolE.
InterPro	IPR001474. GTP_CycHdrlase_I. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. IPR018234. GTP_CycHdrlase_I_CS. [Graphical view]
PANTHER	PTHR11109. PTHR11109. 1 hit.
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
TIGRFAMs	TIGR00063. folE. 1 hit.
PROSITE	PS00859. GTP_CYCLOHYDROL_1_1. 1 hit. PS00860. GTP_CYCLOHYDROL_1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCH1_MYCTU

Accession

Primary (citable) accession number: P64207
Secondary accession number(s): L0TEP6, O06273

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program