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Protein

Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B

Gene

gatB

Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation
ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit BUniRule annotation (EC:6.3.5.-UniRule annotation)
Short name:
Asp/Glu-ADT subunit BUniRule annotation
Gene namesi
Name:gatBUniRule annotation
Ordered Locus Names:SAV1899
OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
Taxonomic identifieri158878 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000002481 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001488341 – 475Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit BAdd BLAST475

Proteomic databases

PaxDbiP64201.
PRIDEiP64201.

2D gel databases

World-2DPAGE0002:P64201.

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

STRINGi158878.SAV1899.

Structurei

Secondary structure

1475
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 14Combined sources12
Beta strandi21 – 28Combined sources8
Turni39 – 43Combined sources5
Helixi53 – 65Combined sources13
Beta strandi72 – 74Combined sources3
Beta strandi77 – 80Combined sources4
Beta strandi89 – 93Combined sources5
Beta strandi95 – 97Combined sources3
Beta strandi99 – 107Combined sources9
Beta strandi109 – 112Combined sources4
Beta strandi114 – 125Combined sources12
Beta strandi129 – 133Combined sources5
Beta strandi136 – 140Combined sources5
Turni142 – 145Combined sources4
Beta strandi147 – 153Combined sources7
Helixi160 – 177Combined sources18
Helixi184 – 186Combined sources3
Beta strandi188 – 199Combined sources12
Beta strandi200 – 202Combined sources3
Beta strandi208 – 212Combined sources5
Helixi217 – 237Combined sources21
Beta strandi243 – 248Combined sources6
Turni250 – 252Combined sources3
Beta strandi255 – 261Combined sources7
Beta strandi262 – 264Combined sources3
Helixi282 – 289Combined sources8
Helixi296 – 305Combined sources10
Helixi311 – 317Combined sources7
Beta strandi318 – 320Combined sources3
Helixi321 – 331Combined sources11
Turni332 – 334Combined sources3
Helixi337 – 345Combined sources9
Helixi347 – 354Combined sources8
Helixi359 – 361Combined sources3
Helixi366 – 377Combined sources12
Beta strandi379 – 381Combined sources3
Helixi383 – 396Combined sources14
Helixi400 – 405Combined sources6
Turni406 – 408Combined sources3
Helixi415 – 428Combined sources14
Helixi430 – 437Combined sources8
Helixi443 – 454Combined sources12
Helixi461 – 474Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DF4X-ray3.20B1-475[»]
2DQNX-ray2.55B1-475[»]
2F2AX-ray2.30B1-475[»]
2G5HX-ray2.50B1-475[»]
2G5IX-ray3.35B1-475[»]
3IP4X-ray1.90B1-475[»]
ProteinModelPortaliP64201.
SMRiP64201.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP64201.

Family & Domainsi

Sequence similaritiesi

Belongs to the GatB/GatE family. GatB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CHT. Bacteria.
COG0064. LUCA.
HOGENOMiHOG000223742.
KOiK02434.
OMAiESADYRY.
PhylomeDBiP64201.

Family and domain databases

Gene3Di1.10.10.410. 1 hit.
HAMAPiMF_00121. GatB. 1 hit.
InterProiIPR004413. Apn/Gln-ADT_bsu.
IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR023168. GatB_Yqey_C.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 1 hit.
PfamiPF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00133. gatB. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P64201-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHFETVIGLE VHVELKTDSK MFSPSPAHFG AEPNSNTNVI DLAYPGVLPV
60 70 80 90 100
VNKRAVDWAM RAAMALNMEI ATESKFDRKN YFYPDNPKAY QISQFDQPIG
110 120 130 140 150
ENGYIDIEVD GETKRIGITR LHMEEDAGKS THKGEYSLVD LNRQGTPLIE
160 170 180 190 200
IVSEPDIRSP KEAYAYLEKL RSIIQYTGVS DVKMEEGSLR CDANISLRPY
210 220 230 240 250
GQEKFGTKAE LKNLNSFNYV RKGLEYEEKR QEEELLNGGE IGQETRRFDE
260 270 280 290 300
STGKTILMRV KEGSDDYRYF PEPDIVPLYI DDAWKERVRQ TIPELPDERK
310 320 330 340 350
AKYVNELGLP AYDAHVLTLT KEMSDFFEST IEHGADVKLT SNWLMGGVNE
360 370 380 390 400
YLNKNQVELL DTKLTPENLA GMIKLIEDGT MSSKIAKKVF PELAAKGGNA
410 420 430 440 450
KQIMEDNGLV QISDEATLLK FVNEALDNNE QSVEDYKNGK GKAMGFLVGQ
460 470
IMKASKGQAN PQLVNQLLKQ ELDKR
Length:475
Mass (Da):53,657
Last modified:October 11, 2004 - v1
Checksum:i96805EE591658154
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA. Translation: BAB58061.1.
RefSeqiWP_000545370.1. NC_002758.2.

Genome annotation databases

EnsemblBacteriaiBAB58061; BAB58061; SAV1899.
GeneIDi28380454.
KEGGisav:SAV1899.
PATRICi19564652. VBIStaAur52173_1966.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA. Translation: BAB58061.1.
RefSeqiWP_000545370.1. NC_002758.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DF4X-ray3.20B1-475[»]
2DQNX-ray2.55B1-475[»]
2F2AX-ray2.30B1-475[»]
2G5HX-ray2.50B1-475[»]
2G5IX-ray3.35B1-475[»]
3IP4X-ray1.90B1-475[»]
ProteinModelPortaliP64201.
SMRiP64201.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi158878.SAV1899.

2D gel databases

World-2DPAGE0002:P64201.

Proteomic databases

PaxDbiP64201.
PRIDEiP64201.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB58061; BAB58061; SAV1899.
GeneIDi28380454.
KEGGisav:SAV1899.
PATRICi19564652. VBIStaAur52173_1966.

Phylogenomic databases

eggNOGiENOG4105CHT. Bacteria.
COG0064. LUCA.
HOGENOMiHOG000223742.
KOiK02434.
OMAiESADYRY.
PhylomeDBiP64201.

Miscellaneous databases

EvolutionaryTraceiP64201.

Family and domain databases

Gene3Di1.10.10.410. 1 hit.
HAMAPiMF_00121. GatB. 1 hit.
InterProiIPR004413. Apn/Gln-ADT_bsu.
IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR023168. GatB_Yqey_C.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 1 hit.
PfamiPF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00133. gatB. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGATB_STAAM
AccessioniPrimary (citable) accession number: P64201
Secondary accession number(s): Q99SY7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: November 2, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.