Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B

Gene

gatB

Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation
ATP + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + phosphate + L-asparaginyl-tRNA(Asn) + L-glutamate.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAUR158878:GJJ5-1954-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit BUniRule annotation (EC:6.3.5.-UniRule annotation)
Short name:
Asp/Glu-ADT subunit BUniRule annotation
Gene namesi
Name:gatBUniRule annotation
Ordered Locus Names:SAV1899
OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
Taxonomic identifieri158878 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000002481 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 475475Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit BPRO_0000148834Add
BLAST

Proteomic databases

PaxDbiP64201.

2D gel databases

World-2DPAGE0002:P64201.

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

STRINGi158878.SAV1899.

Structurei

Secondary structure

1
475
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 1412Combined sources
Beta strandi21 – 288Combined sources
Turni39 – 435Combined sources
Helixi53 – 6513Combined sources
Beta strandi72 – 743Combined sources
Beta strandi77 – 804Combined sources
Beta strandi89 – 935Combined sources
Beta strandi95 – 973Combined sources
Beta strandi99 – 1079Combined sources
Beta strandi109 – 1124Combined sources
Beta strandi114 – 12512Combined sources
Beta strandi129 – 1335Combined sources
Beta strandi136 – 1405Combined sources
Turni142 – 1454Combined sources
Beta strandi147 – 1537Combined sources
Helixi160 – 17718Combined sources
Helixi184 – 1863Combined sources
Beta strandi188 – 19912Combined sources
Beta strandi200 – 2023Combined sources
Beta strandi208 – 2125Combined sources
Helixi217 – 23721Combined sources
Beta strandi243 – 2486Combined sources
Turni250 – 2523Combined sources
Beta strandi255 – 2617Combined sources
Beta strandi262 – 2643Combined sources
Helixi282 – 2898Combined sources
Helixi296 – 30510Combined sources
Helixi311 – 3177Combined sources
Beta strandi318 – 3203Combined sources
Helixi321 – 33111Combined sources
Turni332 – 3343Combined sources
Helixi337 – 3459Combined sources
Helixi347 – 3548Combined sources
Helixi359 – 3613Combined sources
Helixi366 – 37712Combined sources
Beta strandi379 – 3813Combined sources
Helixi383 – 39614Combined sources
Helixi400 – 4056Combined sources
Turni406 – 4083Combined sources
Helixi415 – 42814Combined sources
Helixi430 – 4378Combined sources
Helixi443 – 45412Combined sources
Helixi461 – 47414Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DF4X-ray3.20B1-475[»]
2DQNX-ray2.55B1-475[»]
2F2AX-ray2.30B1-475[»]
2G5HX-ray2.50B1-475[»]
2G5IX-ray3.35B1-475[»]
3IP4X-ray1.90B1-475[»]
ProteinModelPortaliP64201.
SMRiP64201. Positions 1-475.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP64201.

Family & Domainsi

Sequence similaritiesi

Belongs to the GatB/GatE family. GatB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CHT. Bacteria.
COG0064. LUCA.
HOGENOMiHOG000223742.
KOiK02434.
OMAiESADYRY.
PhylomeDBiP64201.

Family and domain databases

Gene3Di1.10.10.410. 1 hit.
HAMAPiMF_00121. GatB. 1 hit.
InterProiIPR004413. Apn/Gln-ADT_bsu.
IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR023168. GatB_Yqey_C.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 1 hit.
PfamiPF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00133. gatB. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P64201-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHFETVIGLE VHVELKTDSK MFSPSPAHFG AEPNSNTNVI DLAYPGVLPV
60 70 80 90 100
VNKRAVDWAM RAAMALNMEI ATESKFDRKN YFYPDNPKAY QISQFDQPIG
110 120 130 140 150
ENGYIDIEVD GETKRIGITR LHMEEDAGKS THKGEYSLVD LNRQGTPLIE
160 170 180 190 200
IVSEPDIRSP KEAYAYLEKL RSIIQYTGVS DVKMEEGSLR CDANISLRPY
210 220 230 240 250
GQEKFGTKAE LKNLNSFNYV RKGLEYEEKR QEEELLNGGE IGQETRRFDE
260 270 280 290 300
STGKTILMRV KEGSDDYRYF PEPDIVPLYI DDAWKERVRQ TIPELPDERK
310 320 330 340 350
AKYVNELGLP AYDAHVLTLT KEMSDFFEST IEHGADVKLT SNWLMGGVNE
360 370 380 390 400
YLNKNQVELL DTKLTPENLA GMIKLIEDGT MSSKIAKKVF PELAAKGGNA
410 420 430 440 450
KQIMEDNGLV QISDEATLLK FVNEALDNNE QSVEDYKNGK GKAMGFLVGQ
460 470
IMKASKGQAN PQLVNQLLKQ ELDKR
Length:475
Mass (Da):53,657
Last modified:October 11, 2004 - v1
Checksum:i96805EE591658154
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA. Translation: BAB58061.1.
RefSeqiWP_000545370.1. NC_002758.2.

Genome annotation databases

EnsemblBacteriaiBAB58061; BAB58061; SAV1899.
KEGGisav:SAV1899.
PATRICi19564652. VBIStaAur52173_1966.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA. Translation: BAB58061.1.
RefSeqiWP_000545370.1. NC_002758.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DF4X-ray3.20B1-475[»]
2DQNX-ray2.55B1-475[»]
2F2AX-ray2.30B1-475[»]
2G5HX-ray2.50B1-475[»]
2G5IX-ray3.35B1-475[»]
3IP4X-ray1.90B1-475[»]
ProteinModelPortaliP64201.
SMRiP64201. Positions 1-475.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi158878.SAV1899.

2D gel databases

World-2DPAGE0002:P64201.

Proteomic databases

PaxDbiP64201.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB58061; BAB58061; SAV1899.
KEGGisav:SAV1899.
PATRICi19564652. VBIStaAur52173_1966.

Phylogenomic databases

eggNOGiENOG4105CHT. Bacteria.
COG0064. LUCA.
HOGENOMiHOG000223742.
KOiK02434.
OMAiESADYRY.
PhylomeDBiP64201.

Enzyme and pathway databases

BioCyciSAUR158878:GJJ5-1954-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP64201.

Family and domain databases

Gene3Di1.10.10.410. 1 hit.
HAMAPiMF_00121. GatB. 1 hit.
InterProiIPR004413. Apn/Gln-ADT_bsu.
IPR017959. Asn/Gln-tRNA_amidoTrfase_suB/E.
IPR006075. Asn/Gln-tRNA_Trfase_suB/E_cat.
IPR018027. Asn/Gln_amidotransferase.
IPR003789. Asn/Gln_tRNA_amidoTrfrase-rel.
IPR023168. GatB_Yqey_C.
IPR017958. Gln-tRNA_amidoTrfase_suB_CS.
[Graphical view]
PANTHERiPTHR11659. PTHR11659. 1 hit.
PfamiPF02934. GatB_N. 1 hit.
PF02637. GatB_Yqey. 1 hit.
[Graphical view]
SMARTiSM00845. GatB_Yqey. 1 hit.
[Graphical view]
SUPFAMiSSF89095. SSF89095. 1 hit.
TIGRFAMsiTIGR00133. gatB. 1 hit.
PROSITEiPS01234. GATB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGATB_STAAM
AccessioniPrimary (citable) accession number: P64201
Secondary accession number(s): Q99SY7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: September 7, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.