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Protein

Glycerol-3-phosphate dehydrogenase [NAD(P)+]

Gene

gpsA

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

sn-glycerol 3-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H.UniRule annotation

Pathwayi: glycerophospholipid metabolism

This protein is involved in the pathway glycerophospholipid metabolism, which is part of Membrane lipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway glycerophospholipid metabolism and in Membrane lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei107NAD; via amide nitrogenUniRule annotation1
Binding sitei107SubstrateUniRule annotation1
Binding sitei139NAD; via amide nitrogenUniRule annotation1
Active sitei190Proton acceptorUniRule annotation1
Binding sitei254NADUniRule annotation1
Binding sitei275NADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi9 – 14NADUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00940.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate dehydrogenase [NAD(P)+]UniRule annotation (EC:1.1.1.94UniRule annotation)
Alternative name(s):
NAD(P)H-dependent glycerol-3-phosphate dehydrogenaseUniRule annotation
Gene namesi
Name:gpsAUniRule annotation
Ordered Locus Names:BMEI0174
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000000419 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001379341 – 326Glycerol-3-phosphate dehydrogenase [NAD(P)+]Add BLAST326

Interactioni

Protein-protein interaction databases

STRINGi224914.BAWG_0023.

Structurei

3D structure databases

ProteinModelPortaliP64186.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni254 – 255Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CSF. Bacteria.
COG0240. LUCA.
HOGENOMiHOG000246853.
KOiK00057.
OMAiRNHTAGR.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_00394. NAD_Glyc3P_dehydrog. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSiPR00077. GPDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS00957. NAD_G3PDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P64186-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTKIAVLGG GAWGTALAAM AAKGGHESWL YARDAETVVA INKDRRNPRY
60 70 80 90 100
LGDITLADGI RASTDAAAVV TGADAVLAVI PAQAMRNGLS ELGTLIPQAS
110 120 130 140 150
PIVLCAKGIE QNTGRLMSEV VAEILPDHRI AALSGPSFAS DVARGLPTAV
160 170 180 190 200
TVACEDANTA DRLAALLSGP AFRCYSTTDL KGVETGGALK NVLAIAAGAA
210 220 230 240 250
IGRGYGASAQ AALVTRGFAE LRRIGQAMSA RPETIMGLSG LGDLMLTCSS
260 270 280 290 300
SQSRNYSYGL ALGRGEDLTS RPLAEGVATA PIAAELCRKH NISAPIIDAV
310 320
GALLDGKITI DEAVTALLNR PLKTED
Length:326
Mass (Da):33,377
Last modified:October 11, 2004 - v1
Checksum:i9853E6E4335CA53C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA. Translation: AAL51356.1.
PIRiAI3273.
RefSeqiWP_002964959.1. NZ_GG703778.1.

Genome annotation databases

EnsemblBacteriaiAAL51356; AAL51356; BMEI0174.
GeneIDi3788737.
KEGGibme:BMEI0174.
bmel:DK63_1262.
PATRICi17795327. VBIBruMel146950_0027.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008917 Genomic DNA. Translation: AAL51356.1.
PIRiAI3273.
RefSeqiWP_002964959.1. NZ_GG703778.1.

3D structure databases

ProteinModelPortaliP64186.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224914.BAWG_0023.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL51356; AAL51356; BMEI0174.
GeneIDi3788737.
KEGGibme:BMEI0174.
bmel:DK63_1262.
PATRICi17795327. VBIBruMel146950_0027.

Phylogenomic databases

eggNOGiENOG4105CSF. Bacteria.
COG0240. LUCA.
HOGENOMiHOG000246853.
KOiK00057.
OMAiRNHTAGR.

Enzyme and pathway databases

UniPathwayiUPA00940.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_00394. NAD_Glyc3P_dehydrog. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSiPR00077. GPDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiPS00957. NAD_G3PDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGPDA_BRUME
AccessioniPrimary (citable) accession number: P64186
Secondary accession number(s): Q8YJB2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: September 7, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.