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P64186 (GPDA_BRUME) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycerol-3-phosphate dehydrogenase [NAD(P)+]

EC=1.1.1.94
Alternative name(s):
NAD(P)H-dependent glycerol-3-phosphate dehydrogenase
Gene names
Name:gpsA
Ordered Locus Names:BMEI0174
OrganismBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) [Complete proteome] [HAMAP]
Taxonomic identifier224914 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length326 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

sn-glycerol 3-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H. HAMAP-Rule MF_00394

Pathway

Membrane lipid metabolism; glycerophospholipid metabolism. HAMAP-Rule MF_00394

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00394.

Sequence similarities

Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 326326Glycerol-3-phosphate dehydrogenase [NAD(P)+] HAMAP-Rule MF_00394
PRO_0000137934

Regions

Nucleotide binding9 – 146NAD By similarity
Region254 – 2552Substrate binding By similarity

Sites

Active site1901Proton acceptor By similarity
Binding site1071NAD; via amide nitrogen By similarity
Binding site1071Substrate By similarity
Binding site1391NAD; via amide nitrogen By similarity
Binding site2541NAD By similarity
Binding site2751NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
P64186 [UniParc].

Last modified October 11, 2004. Version 1.
Checksum: 9853E6E4335CA53C

FASTA32633,377
        10         20         30         40         50         60 
MSTKIAVLGG GAWGTALAAM AAKGGHESWL YARDAETVVA INKDRRNPRY LGDITLADGI 

        70         80         90        100        110        120 
RASTDAAAVV TGADAVLAVI PAQAMRNGLS ELGTLIPQAS PIVLCAKGIE QNTGRLMSEV 

       130        140        150        160        170        180 
VAEILPDHRI AALSGPSFAS DVARGLPTAV TVACEDANTA DRLAALLSGP AFRCYSTTDL 

       190        200        210        220        230        240 
KGVETGGALK NVLAIAAGAA IGRGYGASAQ AALVTRGFAE LRRIGQAMSA RPETIMGLSG 

       250        260        270        280        290        300 
LGDLMLTCSS SQSRNYSYGL ALGRGEDLTS RPLAEGVATA PIAAELCRKH NISAPIIDAV 

       310        320 
GALLDGKITI DEAVTALLNR PLKTED 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008917 Genomic DNA. Translation: AAL51356.1.
PIRAI3273.
RefSeqNP_539092.1. NC_003317.1.

3D structure databases

ProteinModelPortalP64186.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224914.BMEI0174.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL51356; AAL51356; BMEI0174.
GeneID1195886.
KEGGbme:BMEI0174.
PATRIC17795327. VBIBruMel146950_0027.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000246853.
KOK00057.
OMARNHTAGR.
OrthoDBEOG6JDWF4.
ProtClustDBPRK00094.

Enzyme and pathway databases

UniPathwayUPA00940.

Family and domain databases

Gene3D1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPMF_00394. NAD_Glyc3P_dehydrog.
InterProIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11728. PTHR11728. 1 hit.
PfamPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PIRSFPIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTSPR00077. GPDHDRGNASE.
SUPFAMSSF48179. SSF48179. 1 hit.
PROSITEPS00957. NAD_G3PDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGPDA_BRUME
AccessionPrimary (citable) accession number: P64186
Secondary accession number(s): Q8YJB2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: February 19, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Brucella melitensis

Brucella melitensis (strain 16M): entries and gene names