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Protein

Endonuclease 8 1

Gene

nei1

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates (By similarity).By similarity

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

Cofactori

Zn2+PROSITE-ProRule annotationNote: Binds 1 zinc ion per subunit.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2Schiff-base intermediate with DNABy similarity1
Active sitei3Proton donorBy similarity1
Active sitei52Proton donor; for beta-elimination activityBy similarity1
Binding sitei125DNABy similarity1
Binding sitei166DNABy similarity1
Active sitei258Proton donor; for delta-elimination activityBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri234 – 268FPG-typePROSITE-ProRule annotationAdd BLAST35

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Glycosidase, Hydrolase, Lyase, Multifunctional enzyme
Biological processDNA damage, DNA repair
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciMBOV233413:G1GT4-2568-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease 8 1
Alternative name(s):
DNA glycosylase/AP lyase Nei 1 (EC:3.2.2.-)
DNA-(apurinic or apyrimidinic site) lyase Nei 1 (EC:4.2.99.18)
Endonuclease VIII 1
Gene namesi
Name:nei1
Ordered Locus Names:BQ2027_MB2491C
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001419 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001709042 – 268Endonuclease 8 1Add BLAST267

Structurei

3D structure databases

ProteinModelPortaliP64159
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FPG family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri234 – 268FPG-typePROSITE-ProRule annotationAdd BLAST35

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOGENOMiHOG000020880
OMAiMGRPPRV

Family and domain databases

Gene3Di3.20.190.10, 1 hit
InterProiView protein in InterPro
IPR015886 DNA_glyclase/AP_lyase_DNA-bd
IPR015887 DNA_glyclase_Znf_dom_DNA_BS
IPR012319 FPG_cat
IPR035937 MutM-like_N-ter
IPR010979 Ribosomal_S13-like_H2TH
IPR000214 Znf_DNA_glyclase/AP_lyase
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF01149 Fapy_DNA_glyco, 1 hit
PF06831 H2TH, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
SMARTiView protein in SMART
SM00898 Fapy_DNA_glyco, 1 hit
SM01232 H2TH, 1 hit
SUPFAMiSSF46946 SSF46946, 1 hit
SSF81624 SSF81624, 1 hit
PROSITEiView protein in PROSITE
PS01242 ZF_FPG_1, 1 hit
PS51066 ZF_FPG_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P64159-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEGHTLHRL ARLHQRRFAG APVSVSSPQG RFADSASALN GRVLRRASAW
60 70 80 90 100
GKHLFHHYVG GPVVHVHLGL YGTFTEWARP TDGWLPEPAG QVRMRMVGAE
110 120 130 140 150
FGTDLRGPTV CESIDDGEVA DVVARLGPDP LRSDANPSSA WSRITKSRRP
160 170 180 190 200
IGALLMDQTV IAGVGNVYRN ELLFRHRIDP QRPGRGIGEP EFDAAWNDLV
210 220 230 240 250
SLMKVGLRRG KIIVVRPEHD HGLPSYLPDR PRTYVYRRAG EPCRVCGGVI
260
RTALLEGRNV FWCPVCQT
Length:268
Mass (Da):29,714
Last modified:January 23, 2007 - v3
Checksum:i1142DE185BD14F24
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LT708304 Genomic DNA Translation: SIU01106.1
RefSeqiNP_856138.1, NC_002945.3
WP_003412657.1, NC_002945.4

Genome annotation databases

EnsemblBacteriaiCDO43752; CDO43752; Mb2491c
PATRICifig|233413.5.peg.2742

Similar proteinsi

Entry informationi

Entry nameiEND8A_MYCBO
AccessioniPrimary (citable) accession number: P64159
Secondary accession number(s): A0A1R3Y1I4, O53191, X2BKL9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 94 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health