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P64157

- END8B_MYCBO

UniProt

P64157 - END8B_MYCBO

Protein

Endonuclease 8 2

Gene

nei2

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    • Comment

    Functioni

    Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.PROSITE-ProRule annotation

    Catalytic activityi

    Removes damaged bases from DNA, leaving an abasic site.
    The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.PROSITE-ProRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21Schiff-base intermediate with DNAPROSITE-ProRule annotation
    Active sitei3 – 31Proton donorPROSITE-ProRule annotation
    Active sitei51 – 511Proton donor; for beta-elimination activityPROSITE-ProRule annotation
    Binding sitei67 – 671DNABy similarity
    Binding sitei164 – 1641DNABy similarity
    Active sitei245 – 2451Proton donor; for delta-elimination activityPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri221 – 25535FPG-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. damaged DNA binding Source: InterPro
    2. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-EC
    3. hydrolase activity, hydrolyzing N-glycosyl compounds Source: InterPro
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. base-excision repair Source: InterPro
    2. nucleotide-excision repair Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Lyase

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endonuclease 8 2
    Alternative name(s):
    DNA glycosylase/AP lyase Nei 2 (EC:3.2.2.-)
    DNA-(apurinic or apyrimidinic site) lyase Nei 2 (EC:4.2.99.18)
    Endonuclease VIII 2
    Gene namesi
    Name:nei2
    Ordered Locus Names:Mb3325
    OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
    Taxonomic identifieri233413 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    ProteomesiUP000001419: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 255254Endonuclease 8 2PRO_0000170900Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi233413.Mb3325.

    Structurei

    3D structure databases

    ProteinModelPortaliP64157.
    SMRiP64157. Positions 2-254.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the FPG family.PROSITE-ProRule annotation
    Contains 1 FPG-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri221 – 25535FPG-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0266.
    HOGENOMiHOG000020880.
    KOiK05522.
    OMAiIHTHLKM.
    OrthoDBiEOG6QP131.

    Family and domain databases

    InterProiIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
    IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
    IPR012319. DNA_glycosylase/AP_lyase_cat.
    IPR010979. Ribosomal_S13-like_H2TH.
    IPR000214. Znf_DNA_glyclase/AP_lyase.
    [Graphical view]
    PfamiPF01149. Fapy_DNA_glyco. 1 hit.
    PF06831. H2TH. 1 hit.
    [Graphical view]
    SMARTiSM00898. Fapy_DNA_glyco. 1 hit.
    [Graphical view]
    SUPFAMiSSF46946. SSF46946. 1 hit.
    SSF81624. SSF81624. 1 hit.
    PROSITEiPS51068. FPG_CAT. 1 hit.
    PS01242. ZF_FPG_1. 1 hit.
    PS51066. ZF_FPG_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P64157-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPEGDTVWHT AATLRRHLAG RTLTRCDIRV PRFAAVDLTG EVVDEVISRG    50
    KHLFIRTGTA SIHSHLQMDG SWRVGNRPVR VDHRARIILE ANQQEQAIRV 100
    VGVDLGLLEV IDRHNDGAVV AHLGPDLLAD DWDPQRAAAN LIVAPDRPIA 150
    EALLDQRVLA GIGNVYCNEL CFVSGVLPTA PVSAVADPRR LVTRARDMLW 200
    VNRFRWNRCT TGDTRAGRRL WVYGRAGQGC RRCGTLIAYD TTDERVRYWC 250
    PACQR 255
    Length:255
    Mass (Da):28,526
    Last modified:January 23, 2007 - v3
    Checksum:i2C97B16CD7CD4002
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX248333 Genomic DNA. Translation: CDO44597.1.
    RefSeqiNP_856970.1. NC_002945.3.

    Genome annotation databases

    EnsemblBacteriaiCAD95417; CAD95417; Mb3325.
    GeneIDi1093989.
    KEGGimbo:Mb3325.
    PATRICi18009054. VBIMycBov88188_3655.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX248333 Genomic DNA. Translation: CDO44597.1 .
    RefSeqi NP_856970.1. NC_002945.3.

    3D structure databases

    ProteinModelPortali P64157.
    SMRi P64157. Positions 2-254.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 233413.Mb3325.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAD95417 ; CAD95417 ; Mb3325 .
    GeneIDi 1093989.
    KEGGi mbo:Mb3325.
    PATRICi 18009054. VBIMycBov88188_3655.

    Phylogenomic databases

    eggNOGi COG0266.
    HOGENOMi HOG000020880.
    KOi K05522.
    OMAi IHTHLKM.
    OrthoDBi EOG6QP131.

    Family and domain databases

    InterProi IPR015886. DNA_glyclase/AP_lyase_DNA-bd.
    IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
    IPR012319. DNA_glycosylase/AP_lyase_cat.
    IPR010979. Ribosomal_S13-like_H2TH.
    IPR000214. Znf_DNA_glyclase/AP_lyase.
    [Graphical view ]
    Pfami PF01149. Fapy_DNA_glyco. 1 hit.
    PF06831. H2TH. 1 hit.
    [Graphical view ]
    SMARTi SM00898. Fapy_DNA_glyco. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46946. SSF46946. 1 hit.
    SSF81624. SSF81624. 1 hit.
    PROSITEi PS51068. FPG_CAT. 1 hit.
    PS01242. ZF_FPG_1. 1 hit.
    PS51066. ZF_FPG_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-935 / AF2122/97.

    Entry informationi

    Entry nameiEND8B_MYCBO
    AccessioniPrimary (citable) accession number: P64157
    Secondary accession number(s): P96902, X2BMS5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 67 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3