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Protein

Endonuclease 8 2

Gene

nei2

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.PROSITE-ProRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.PROSITE-ProRule annotation

Cofactori

Zn2+PROSITE-ProRule annotationNote: Binds 1 zinc ion per subunit.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2Schiff-base intermediate with DNAPROSITE-ProRule annotation1
Active sitei3Proton donorPROSITE-ProRule annotation1
Active sitei51Proton donor; for beta-elimination activityPROSITE-ProRule annotation1
Binding sitei67DNABy similarity1
Binding sitei164DNABy similarity1
Active sitei245Proton donor; for delta-elimination activityPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri221 – 255FPG-typePROSITE-ProRule annotationAdd BLAST35

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Glycosidase, Hydrolase, Lyase, Multifunctional enzyme
Biological processDNA damage, DNA repair
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciMBOV233413:G1GT4-3493-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease 8 2
Alternative name(s):
DNA glycosylase/AP lyase Nei 2 (EC:3.2.2.-)
DNA-(apurinic or apyrimidinic site) lyase Nei 2 (EC:4.2.99.18)
Endonuclease VIII 2
Gene namesi
Name:nei2
Ordered Locus Names:BQ2027_MB3325
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001419 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001709002 – 255Endonuclease 8 2Add BLAST254

Structurei

3D structure databases

ProteinModelPortaliP64157
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FPG family.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri221 – 255FPG-typePROSITE-ProRule annotationAdd BLAST35

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOGENOMiHOG000020880
OMAiEILWQAQ

Family and domain databases

Gene3Di3.20.190.10, 1 hit
InterProiView protein in InterPro
IPR015886 DNA_glyclase/AP_lyase_DNA-bd
IPR015887 DNA_glyclase_Znf_dom_DNA_BS
IPR012319 FPG_cat
IPR035937 MutM-like_N-ter
IPR010979 Ribosomal_S13-like_H2TH
IPR000214 Znf_DNA_glyclase/AP_lyase
PfamiView protein in Pfam
PF01149 Fapy_DNA_glyco, 1 hit
PF06831 H2TH, 1 hit
SMARTiView protein in SMART
SM00898 Fapy_DNA_glyco, 1 hit
SM01232 H2TH, 1 hit
SUPFAMiSSF46946 SSF46946, 1 hit
SSF81624 SSF81624, 1 hit
PROSITEiView protein in PROSITE
PS51068 FPG_CAT, 1 hit
PS01242 ZF_FPG_1, 1 hit
PS51066 ZF_FPG_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P64157-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEGDTVWHT AATLRRHLAG RTLTRCDIRV PRFAAVDLTG EVVDEVISRG
60 70 80 90 100
KHLFIRTGTA SIHSHLQMDG SWRVGNRPVR VDHRARIILE ANQQEQAIRV
110 120 130 140 150
VGVDLGLLEV IDRHNDGAVV AHLGPDLLAD DWDPQRAAAN LIVAPDRPIA
160 170 180 190 200
EALLDQRVLA GIGNVYCNEL CFVSGVLPTA PVSAVADPRR LVTRARDMLW
210 220 230 240 250
VNRFRWNRCT TGDTRAGRRL WVYGRAGQGC RRCGTLIAYD TTDERVRYWC

PACQR
Length:255
Mass (Da):28,526
Last modified:January 23, 2007 - v3
Checksum:i2C97B16CD7CD4002
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
LT708304 Genomic DNA Translation: SIU01954.1
RefSeqiNP_856970.1, NC_002945.3
WP_003900006.1, NC_002945.4

Genome annotation databases

EnsemblBacteriaiCDO44597; CDO44597; Mb3325
PATRICifig|233413.5.peg.3655

Similar proteinsi

Entry informationi

Entry nameiEND8B_MYCBO
AccessioniPrimary (citable) accession number: P64157
Secondary accession number(s): A0A1R3Y3Q6, P96902, X2BMS5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 89 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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