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P64157

- END8B_MYCBO

UniProt

P64157 - END8B_MYCBO

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Protein

Endonuclease 8 2

Gene

nei2

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.PROSITE-ProRule annotation

Catalytic activityi

Removes damaged bases from DNA, leaving an abasic site.
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.PROSITE-ProRule annotation

Cofactori

Zn2+PROSITE-ProRule annotationNote: Binds 1 zinc ion per subunit.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Schiff-base intermediate with DNAPROSITE-ProRule annotation
Active sitei3 – 31Proton donorPROSITE-ProRule annotation
Active sitei51 – 511Proton donor; for beta-elimination activityPROSITE-ProRule annotation
Binding sitei67 – 671DNABy similarity
Binding sitei164 – 1641DNABy similarity
Active sitei245 – 2451Proton donor; for delta-elimination activityPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri221 – 25535FPG-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. damaged DNA binding Source: InterPro
  2. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-EC
  3. hydrolase activity, hydrolyzing N-glycosyl compounds Source: InterPro
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. base-excision repair Source: InterPro
  2. nucleotide-excision repair Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Lyase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease 8 2
Alternative name(s):
DNA glycosylase/AP lyase Nei 2 (EC:3.2.2.-)
DNA-(apurinic or apyrimidinic site) lyase Nei 2 (EC:4.2.99.18)
Endonuclease VIII 2
Gene namesi
Name:nei2
Ordered Locus Names:Mb3325
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001419: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 255254Endonuclease 8 2PRO_0000170900Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi233413.Mb3325.

Structurei

3D structure databases

ProteinModelPortaliP64157.
SMRiP64157. Positions 2-254.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FPG family.PROSITE-ProRule annotation
Contains 1 FPG-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri221 – 25535FPG-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG0266.
HOGENOMiHOG000020880.
KOiK05522.
OMAiIHTHLKM.
OrthoDBiEOG6QP131.

Family and domain databases

InterProiIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
[Graphical view]
PfamiPF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
[Graphical view]
SMARTiSM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view]
SUPFAMiSSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
PROSITEiPS51068. FPG_CAT. 1 hit.
PS01242. ZF_FPG_1. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P64157-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPEGDTVWHT AATLRRHLAG RTLTRCDIRV PRFAAVDLTG EVVDEVISRG
60 70 80 90 100
KHLFIRTGTA SIHSHLQMDG SWRVGNRPVR VDHRARIILE ANQQEQAIRV
110 120 130 140 150
VGVDLGLLEV IDRHNDGAVV AHLGPDLLAD DWDPQRAAAN LIVAPDRPIA
160 170 180 190 200
EALLDQRVLA GIGNVYCNEL CFVSGVLPTA PVSAVADPRR LVTRARDMLW
210 220 230 240 250
VNRFRWNRCT TGDTRAGRRL WVYGRAGQGC RRCGTLIAYD TTDERVRYWC

PACQR
Length:255
Mass (Da):28,526
Last modified:January 23, 2007 - v3
Checksum:i2C97B16CD7CD4002
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO44597.1.
RefSeqiNP_856970.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCAD95417; CAD95417; Mb3325.
GeneIDi1093989.
KEGGimbo:Mb3325.
PATRICi18009054. VBIMycBov88188_3655.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO44597.1 .
RefSeqi NP_856970.1. NC_002945.3.

3D structure databases

ProteinModelPortali P64157.
SMRi P64157. Positions 2-254.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 233413.Mb3325.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD95417 ; CAD95417 ; Mb3325 .
GeneIDi 1093989.
KEGGi mbo:Mb3325.
PATRICi 18009054. VBIMycBov88188_3655.

Phylogenomic databases

eggNOGi COG0266.
HOGENOMi HOG000020880.
KOi K05522.
OMAi IHTHLKM.
OrthoDBi EOG6QP131.

Family and domain databases

InterProi IPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
[Graphical view ]
Pfami PF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
[Graphical view ]
SMARTi SM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view ]
SUPFAMi SSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
PROSITEi PS51068. FPG_CAT. 1 hit.
PS01242. ZF_FPG_1. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-935 / AF2122/97.

Entry informationi

Entry nameiEND8B_MYCBO
AccessioniPrimary (citable) accession number: P64157
Secondary accession number(s): P96902, X2BMS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 68 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3