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P64157 (END8B_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endonuclease 8 2
Alternative name(s):
DNA glycosylase/AP lyase Nei 2
EC=3.2.2.-
DNA-(apurinic or apyrimidinic site) lyase Nei 2
EC=4.2.99.18
Endonuclease VIII 2
Gene names
Name:nei2
Ordered Locus Names:Mb3325
OrganismMycobacterium bovis (strain ATCC BAA-935 / AF2122/97) [Complete proteome] [HAMAP]
Taxonomic identifier233413 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

Protein attributes

Sequence length255 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates By similarity.

Catalytic activity

Removes damaged bases from DNA, leaving an abasic site.

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Sequence similarities

Belongs to the FPG family.

Contains 1 FPG-type zinc finger.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 255254Endonuclease 8 2
PRO_0000170900

Regions

Zinc finger221 – 25535FPG-type

Sites

Active site21Schiff-base intermediate with DNA By similarity
Active site31Proton donor By similarity
Active site511Proton donor; for beta-elimination activity By similarity
Active site2451Proton donor; for delta-elimination activity By similarity
Binding site671DNA By similarity
Binding site1641DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
P64157 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 2C97B16CD7CD4002

FASTA25528,526
        10         20         30         40         50         60 
MPEGDTVWHT AATLRRHLAG RTLTRCDIRV PRFAAVDLTG EVVDEVISRG KHLFIRTGTA 

        70         80         90        100        110        120 
SIHSHLQMDG SWRVGNRPVR VDHRARIILE ANQQEQAIRV VGVDLGLLEV IDRHNDGAVV 

       130        140        150        160        170        180 
AHLGPDLLAD DWDPQRAAAN LIVAPDRPIA EALLDQRVLA GIGNVYCNEL CFVSGVLPTA 

       190        200        210        220        230        240 
PVSAVADPRR LVTRARDMLW VNRFRWNRCT TGDTRAGRRL WVYGRAGQGC RRCGTLIAYD 

       250 
TTDERVRYWC PACQR 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX248345 Genomic DNA. Translation: CAD95417.1.
RefSeqNP_856970.1. NC_002945.3.

3D structure databases

ProteinModelPortalP64157.
SMRP64157. Positions 2-254.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING233413.Mb3325.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD95417; CAD95417; Mb3325.
GeneID1093989.
KEGGmbo:Mb3325.
PATRIC18009054. VBIMycBov88188_3655.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0266.
HOGENOMHOG000020880.
KOK05522.
OMAGPDVLDM.
OrthoDBEOG6QP131.
ProtClustDBCLSK792413.

Family and domain databases

InterProIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR015887. DNA_glyclase_Znf_dom_DNA_BS.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
[Graphical view]
PfamPF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
[Graphical view]
SMARTSM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view]
SUPFAMSSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
PROSITEPS51068. FPG_CAT. 1 hit.
PS01242. ZF_FPG_1. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameEND8B_MYCBO
AccessionPrimary (citable) accession number: P64157
Secondary accession number(s): P96902
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: January 23, 2007
Last modified: November 13, 2013
This is version 64 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families