Endonuclease 8 2 - Mycobacterium tuberculosis

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P64156 (END8B_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 64. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Endonuclease 8 2

Alternative name(s):
DNA glycosylase/AP lyase Nei 2
EC=3.2.2.-
DNA-(apurinic or apyrimidinic site) lyase Nei 2
EC=4.2.99.18
Endonuclease VIII 2

Gene names

Name:	nei2
Synonyms:	nei1
Ordered Locus Names:	Rv3297, MT3396
ORF Names:	MTCY71.37

Organism

Mycobacterium tuberculosis [Reference proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates By similarity. Complements an E.coli nei nth double mutant (Ref.4). Ref.3 Ref.4
Catalytic activity	Removes damaged bases from DNA, leaving an abasic site. The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Sequence similarities	Belongs to the FPG family. Contains 1 FPG-type zinc finger.
Caution	There are 2 paralogs in M.tuberculosis, in a reference this gene is called nei1 (Ref.3).

Ontologies

Keywords
Biological process	DNA damage DNA repair
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Glycosidase Hydrolase Lyase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from direct assay Ref.4. Source: MTBBASE base-excision repair Inferred from electronic annotation. Source: InterPro nucleotide-excision repair Inferred from electronic annotation. Source: InterPro
Molecular_function	DNA N-glycosylase activity Inferred from direct assay Ref.4. Source: MTBBASE DNA-(apurinic or apyrimidinic site) lyase activity Inferred from electronic annotation. Source: EC damaged DNA binding Inferred from electronic annotation. Source: InterPro double-stranded DNA binding Inferred from direct assay Ref.4. Source: MTBBASE zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed By similarity

Chain

2 – 255

254

Endonuclease 8 2

PRO_0000170901

Regions

Zinc finger

221 – 255

FPG-type

Sites

Active site

Schiff-base intermediate with DNA By similarity

Active site

Proton donor By similarity

Active site

Proton donor; for beta-elimination activity By similarity

Active site

245

Proton donor; for delta-elimination activity By similarity

Binding site

DNA By similarity

Binding site

164

DNA By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P64156 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 2C97B16CD7CD4002
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FASTA

255

28,526

        10         20         30         40         50         60 
MPEGDTVWHT AATLRRHLAG RTLTRCDIRV PRFAAVDLTG EVVDEVISRG KHLFIRTGTA 

        70         80         90        100        110        120 
SIHSHLQMDG SWRVGNRPVR VDHRARIILE ANQQEQAIRV VGVDLGLLEV IDRHNDGAVV 

       130        140        150        160        170        180 
AHLGPDLLAD DWDPQRAAAN LIVAPDRPIA EALLDQRVLA GIGNVYCNEL CFVSGVLPTA 

       190        200        210        220        230        240 
PVSAVADPRR LVTRARDMLW VNRFRWNRCT TGDTRAGRRL WVYGRAGQGC RRCGTLIAYD 

       250 
TTDERVRYWC PACQR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. Barrell B.G. Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"Novel DNA glycosylases from Mycobacterium tuberculosis." Sidorenko V.S., Rot M.A., Filipenko M.L., Nevinsky G.A., Zharkov D.O. Biokhimiia 73:442-450(2008) [PubMed] [Europe PMC] [Abstract] Cited for: DISCUSSION OF FUNCTION.
[4]	"The oxidative DNA glycosylases of Mycobacterium tuberculosis exhibit different substrate preferences from their Escherichia coli counterparts." Guo Y., Bandaru V., Jaruga P., Zhao X., Burrows C.J., Iwai S., Dizdaroglu M., Bond J.P., Wallace S.S. DNA Repair 9:177-190(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Strain: ATCC 25618 / H37Rv.
[5]	"Base excision and nucleotide excision repair pathways in mycobacteria." Kurthkoti K., Varshney U. Tuberculosis 91:533-543(2011) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842582 Genomic DNA. Translation: CAB07061.1. AE000516 Genomic DNA. Translation: AAK47739.1. AL123456 Genomic DNA. Translation: CCP46116.1.
PIR	B70982.
RefSeq	NP_217814.1. NC_000962.3. NP_337925.1. NC_002755.2. YP_006516774.1. NC_018143.1.
3D structure databases
ProteinModelPortal	P64156.
SMR	P64156. Positions 2-254.
ModBase	Search...
Protein-protein interaction databases
STRING	83332.Rv3297.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000002647; EBMYCP00000002647; EBMYCG00000002645. EBMYCT00000071901; EBMYCP00000069960; EBMYCG00000071896.
GeneID	13318120. 887937. 926657.
KEGG	mtc:MT3396. mtu:Rv3297. mtv:RVBD_3297.
PATRIC	18129220. VBIMycTub22151_3706.
Organism-specific databases
TubercuList	Rv3297.
Phylogenomic databases
eggNOG	COG0266.
HOGENOM	HOG000020880.
KO	K05522.
OMA	WFAFPEL.
ProtClustDB	CLSK792413.
Family and domain databases
InterPro	IPR015886. DNA_glyclase/AP_lyase_DNA-bd. IPR015887. DNA_glyclase_Znf_dom_DNA_BS. IPR012319. DNA_glycosylase/AP_lyase_cat. IPR010979. Ribosomal_S13-like_H2TH. IPR000214. Znf_DNA_glyclase/AP_lyase. [Graphical view]
Pfam	PF01149. Fapy_DNA_glyco. 1 hit. PF06831. H2TH. 1 hit. [Graphical view]
SMART	SM00898. Fapy_DNA_glyco. 1 hit. [Graphical view]
SUPFAM	SSF81624. Form_DNAglyc_cat. 1 hit. SSF46946. Ribosomal_H2TH. 1 hit.
PROSITE	PS51068. FPG_CAT. 1 hit. PS01242. ZF_FPG_1. 1 hit. PS51066. ZF_FPG_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

END8B_MYCTU

Accession

Primary (citable) accession number: P64156
Secondary accession number(s): L0TDQ8, P96902

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 64 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh

Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents