P64143 (HPPK_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase
EC=2.7.6.3

Alternative name(s):
6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase
Short name=PPPK
7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase
Short name=HPPK

Gene names

Name:	folK
Ordered Locus Names:	Rv3606c, MT3711
ORF Names:	MTCY07H7B.16

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine = AMP + (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate: step 4/4.
Sequence similarities	Belongs to the HPPK family.

Ontologies

Keywords
Biological process	Folate biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	folic acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase		PRO_0000168255

Sequences

	Sequence		Length	Mass (Da)
	P64143 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: D14AE440760CDCF4	FASTA	188	20,732
	10 20 30 40 50 60 MTRVVLSVGS NLGDRLARLR SVADGLGDAL IAASPIYEAD PWGGVEQGQF LNAVLIADDP 70 80 90 100 110 120 TCEPREWLRR AQEFERAAGR VRGQRWGPRN LDVDLIACYQ TSATEALVEV TARENHLTLP 130 140 150 160 170 180 HPLAHLRAFV LIPWIAVDPT AQLTVAGCPR PVTRLLAELE PADRDSVRLF RPSFDLNSRH PVSRAPES

References

[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842583 Genomic DNA. Translation: CAB08938.1. AE000516 Genomic DNA. Translation: AAK48069.1.
PIR	G70955.
RefSeq	NP_218123.1. NC_000962.2. NP_338255.1. NC_002755.2.
3D structure databases
ProteinModelPortal	P64143.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000001710; EBMYCP00000001710; EBMYCG00000001709. EBMYCT00000070688; EBMYCP00000068747; EBMYCG00000070683.
GeneID	885848. 922783.
GenomeReviews	Gene locus MT3711 in contig AE000516_GR. Gene locus Rv3606c in contig AL123456_GR.
KEGG	mtc:MT3711. mtu:Rv3606c.
PATRIC	18129913. VBIMycTub22151_4051.
TIGR	MT3711.
Organism-specific databases
TubercuList	Rv3606c.
Phylogenomic databases
GeneTree	EBGT00050000016430.
HOGENOM	HBG662565.
OMA	LAADFIY.
PhylomeDB	P64143.
ProtClustDB	CLSK792592.
Family and domain databases
InterPro	IPR000550. Hppk. [Graphical view]
Gene3D	G3DSA:3.30.70.560. Hppk. 1 hit.
KO	K00950.
Pfam	PF01288. HPPK. 1 hit. [Graphical view]
SUPFAM	SSF55083. Hppk. 1 hit.
TIGRFAMs	TIGR01498. FolK. 1 hit.
PROSITE	PS00794. HPPK. 1 hit. [Graphical view]
ProtoNet	Search...
Other
DrugBank	DB00233. Aminosalicylic Acid.

Entry information

Entry name

HPPK_MYCTU

Accession

Primary (citable) accession number: P64143
Secondary accession number(s): O06276

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program