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Protein

Dihydropteroate synthase

Gene

folP

Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

DHPS catalyzes the formation of the immediate precursor of folic acid. It is implicated in resistance to sulfonamide.

Catalytic activityi

(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit. Magnesium is required for activity, even if it interacts primarily with the substrate.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi11 – 111MagnesiumBy similarity
Binding sitei19 – 191SubstrateBy similarity
Binding sitei84 – 841SubstrateBy similarity
Binding sitei103 – 1031SubstrateBy similarity
Binding sitei167 – 1671SubstrateBy similarity
Binding sitei203 – 2031SubstrateBy similarity
Binding sitei239 – 2391SubstrateBy similarity
Binding sitei241 – 2411SubstrateBy similarity

GO - Molecular functioni

  1. dihydropteroate synthase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. folic acid biosynthetic process Source: UniProtKB-KW
  2. response to antibiotic Source: UniProtKB-KW
  3. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Antibiotic resistance, Folate biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciSAUR158878:GJJ5-518-MONOMER.
UniPathwayiUPA00077; UER00156.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydropteroate synthase (EC:2.5.1.15)
Short name:
DHPS
Alternative name(s):
Dihydropteroate pyrophosphorylase
Gene namesi
Name:folP
Ordered Locus Names:SAV0514
OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
Taxonomic identifieri158878 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000002481: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 267267Dihydropteroate synthasePRO_0000168221Add
BLAST

Proteomic databases

PRIDEiP64141.

2D gel databases

World-2DPAGE0002:P64141.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi158878.SAV0514.

Structurei

Secondary structure

1
267
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 117Combined sources
Helixi25 – 3814Combined sources
Beta strandi42 – 487Combined sources
Helixi60 – 7415Combined sources
Beta strandi77 – 848Combined sources
Helixi88 – 9710Combined sources
Beta strandi101 – 1044Combined sources
Turni105 – 1084Combined sources
Helixi114 – 1207Combined sources
Beta strandi124 – 1285Combined sources
Helixi139 – 15618Combined sources
Helixi161 – 1633Combined sources
Beta strandi164 – 1674Combined sources
Helixi176 – 1838Combined sources
Helixi186 – 1905Combined sources
Beta strandi196 – 1983Combined sources
Helixi204 – 2107Combined sources
Helixi217 – 2193Combined sources
Helixi220 – 23314Combined sources
Beta strandi237 – 2415Combined sources
Helixi243 – 26220Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HB7X-ray1.95A/B1-267[»]
ProteinModelPortaliP64141.
SMRiP64141. Positions 2-265.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 251251Pterin-bindingPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni51 – 522Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the DHPS family.Curated
Contains 1 pterin-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0294.
HOGENOMiHOG000217509.
KOiK00796.
OMAiSIDTYHA.
OrthoDBiEOG67T5P5.
PhylomeDBiP64141.

Family and domain databases

Gene3Di3.20.20.20. 1 hit.
InterProiIPR006390. DHP_synth.
IPR011005. Dihydropteroate_synth-like.
IPR000489. Pterin-binding.
[Graphical view]
PfamiPF00809. Pterin_bind. 1 hit.
[Graphical view]
SUPFAMiSSF51717. SSF51717. 1 hit.
TIGRFAMsiTIGR01496. DHPS. 1 hit.
PROSITEiPS00792. DHPS_1. 1 hit.
PS00793. DHPS_2. 1 hit.
PS50972. PTERIN_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P64141-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKTKIMGIL NVTPDSFSDG GKFNNVETAI NRVKAMIDEG ADIIDVGGVS
60 70 80 90 100
TRPGHEMVTL EEELNRVLPV VEAIVGFDVK ISVDTFRSEV AEACLKLGVD
110 120 130 140 150
MINDQWAGLY DHRMFQIVAK YDAEIILMHN GNGNRDEPVV EEMLTSLLAQ
160 170 180 190 200
AHQAKIAGIP SNKIWLDPGI GFAKTRNEEA EVMARLDELV ATEYPVLLAT
210 220 230 240 250
SRKRFTKEMM GYDTTPVERD EVTAATTAYG IMKGVRAVRV HNVELNAKLA
260
KGIDFLKENE NARHNLS
Length:267
Mass (Da):29,533
Last modified:October 11, 2004 - v1
Checksum:iA2E41F5C5544D86C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA. Translation: BAB56676.1.
RefSeqiNP_371038.1. NC_002758.2.

Genome annotation databases

EnsemblBacteriaiBAB56676; BAB56676; SAV0514.
GeneIDi1120474.
KEGGisav:SAV0514.
PATRICi19561650. VBIStaAur52173_0518.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000017 Genomic DNA. Translation: BAB56676.1.
RefSeqiNP_371038.1. NC_002758.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HB7X-ray1.95A/B1-267[»]
ProteinModelPortaliP64141.
SMRiP64141. Positions 2-265.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi158878.SAV0514.

2D gel databases

World-2DPAGE0002:P64141.

Proteomic databases

PRIDEiP64141.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB56676; BAB56676; SAV0514.
GeneIDi1120474.
KEGGisav:SAV0514.
PATRICi19561650. VBIStaAur52173_0518.

Phylogenomic databases

eggNOGiCOG0294.
HOGENOMiHOG000217509.
KOiK00796.
OMAiSIDTYHA.
OrthoDBiEOG67T5P5.
PhylomeDBiP64141.

Enzyme and pathway databases

UniPathwayiUPA00077; UER00156.
BioCyciSAUR158878:GJJ5-518-MONOMER.

Family and domain databases

Gene3Di3.20.20.20. 1 hit.
InterProiIPR006390. DHP_synth.
IPR011005. Dihydropteroate_synth-like.
IPR000489. Pterin-binding.
[Graphical view]
PfamiPF00809. Pterin_bind. 1 hit.
[Graphical view]
SUPFAMiSSF51717. SSF51717. 1 hit.
TIGRFAMsiTIGR01496. DHPS. 1 hit.
PROSITEiPS00792. DHPS_1. 1 hit.
PS00793. DHPS_2. 1 hit.
PS50972. PTERIN_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Mu50 / ATCC 700699.

Entry informationi

Entry nameiDHPS_STAAM
AccessioniPrimary (citable) accession number: P64141
Secondary accession number(s): Q99W89
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: January 7, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.