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P64141

- DHPS_STAAM

UniProt

P64141 - DHPS_STAAM

Protein

Dihydropteroate synthase

Gene

folP

Organism
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    DHPS catalyzes the formation of the immediate precursor of folic acid. It is implicated in resistance to sulfonamide.

    Catalytic activityi

    (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate.

    Cofactori

    Binds 1 magnesium ion per subunit. Magnesium is required for activity, even if it interacts primarily with the substrate By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi11 – 111MagnesiumBy similarity
    Binding sitei19 – 191SubstrateBy similarity
    Binding sitei84 – 841SubstrateBy similarity
    Binding sitei103 – 1031SubstrateBy similarity
    Binding sitei167 – 1671SubstrateBy similarity
    Binding sitei203 – 2031SubstrateBy similarity
    Binding sitei239 – 2391SubstrateBy similarity
    Binding sitei241 – 2411SubstrateBy similarity

    GO - Molecular functioni

    1. dihydropteroate synthase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. folic acid biosynthetic process Source: UniProtKB-KW
    2. response to antibiotic Source: UniProtKB-KW
    3. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Antibiotic resistance, Folate biosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciSAUR158878:GJJ5-518-MONOMER.
    UniPathwayiUPA00077; UER00156.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dihydropteroate synthase (EC:2.5.1.15)
    Short name:
    DHPS
    Alternative name(s):
    Dihydropteroate pyrophosphorylase
    Gene namesi
    Name:folP
    Ordered Locus Names:SAV0514
    OrganismiStaphylococcus aureus (strain Mu50 / ATCC 700699)
    Taxonomic identifieri158878 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000002481: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 267267Dihydropteroate synthasePRO_0000168221Add
    BLAST

    Proteomic databases

    PRIDEiP64141.

    2D gel databases

    World-2DPAGE0002:P64141.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi158878.SAV0514.

    Structurei

    Secondary structure

    1
    267
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi5 – 117
    Helixi25 – 3814
    Beta strandi42 – 487
    Helixi60 – 7415
    Beta strandi77 – 848
    Helixi88 – 9710
    Beta strandi101 – 1044
    Turni105 – 1084
    Helixi114 – 1207
    Beta strandi124 – 1285
    Helixi139 – 15618
    Helixi161 – 1633
    Beta strandi164 – 1674
    Helixi176 – 1838
    Helixi186 – 1905
    Beta strandi196 – 1983
    Helixi204 – 2107
    Helixi217 – 2193
    Helixi220 – 23314
    Beta strandi237 – 2415
    Helixi243 – 26220

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4HB7X-ray1.95A/B1-267[»]
    ProteinModelPortaliP64141.
    SMRiP64141. Positions 2-265.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 251251Pterin-bindingPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni51 – 522Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the DHPS family.Curated
    Contains 1 pterin-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0294.
    HOGENOMiHOG000217509.
    KOiK00796.
    OMAiDCWISVD.
    OrthoDBiEOG67T5P5.
    PhylomeDBiP64141.

    Family and domain databases

    Gene3Di3.20.20.20. 1 hit.
    InterProiIPR006390. DHP_synth.
    IPR011005. Dihydropteroate_synth-like.
    IPR000489. Pterin-binding.
    [Graphical view]
    PfamiPF00809. Pterin_bind. 1 hit.
    [Graphical view]
    SUPFAMiSSF51717. SSF51717. 1 hit.
    TIGRFAMsiTIGR01496. DHPS. 1 hit.
    PROSITEiPS00792. DHPS_1. 1 hit.
    PS00793. DHPS_2. 1 hit.
    PS50972. PTERIN_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P64141-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTKTKIMGIL NVTPDSFSDG GKFNNVETAI NRVKAMIDEG ADIIDVGGVS    50
    TRPGHEMVTL EEELNRVLPV VEAIVGFDVK ISVDTFRSEV AEACLKLGVD 100
    MINDQWAGLY DHRMFQIVAK YDAEIILMHN GNGNRDEPVV EEMLTSLLAQ 150
    AHQAKIAGIP SNKIWLDPGI GFAKTRNEEA EVMARLDELV ATEYPVLLAT 200
    SRKRFTKEMM GYDTTPVERD EVTAATTAYG IMKGVRAVRV HNVELNAKLA 250
    KGIDFLKENE NARHNLS 267
    Length:267
    Mass (Da):29,533
    Last modified:October 11, 2004 - v1
    Checksum:iA2E41F5C5544D86C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000017 Genomic DNA. Translation: BAB56676.1.
    RefSeqiNP_371038.1. NC_002758.2.

    Genome annotation databases

    EnsemblBacteriaiBAB56676; BAB56676; SAV0514.
    GeneIDi1120474.
    KEGGisav:SAV0514.
    PATRICi19561650. VBIStaAur52173_0518.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000017 Genomic DNA. Translation: BAB56676.1 .
    RefSeqi NP_371038.1. NC_002758.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4HB7 X-ray 1.95 A/B 1-267 [» ]
    ProteinModelPortali P64141.
    SMRi P64141. Positions 2-265.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 158878.SAV0514.

    2D gel databases

    World-2DPAGE 0002:P64141.

    Proteomic databases

    PRIDEi P64141.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB56676 ; BAB56676 ; SAV0514 .
    GeneIDi 1120474.
    KEGGi sav:SAV0514.
    PATRICi 19561650. VBIStaAur52173_0518.

    Phylogenomic databases

    eggNOGi COG0294.
    HOGENOMi HOG000217509.
    KOi K00796.
    OMAi DCWISVD.
    OrthoDBi EOG67T5P5.
    PhylomeDBi P64141.

    Enzyme and pathway databases

    UniPathwayi UPA00077 ; UER00156 .
    BioCyci SAUR158878:GJJ5-518-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.20. 1 hit.
    InterProi IPR006390. DHP_synth.
    IPR011005. Dihydropteroate_synth-like.
    IPR000489. Pterin-binding.
    [Graphical view ]
    Pfami PF00809. Pterin_bind. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51717. SSF51717. 1 hit.
    TIGRFAMsi TIGR01496. DHPS. 1 hit.
    PROSITEi PS00792. DHPS_1. 1 hit.
    PS00793. DHPS_2. 1 hit.
    PS50972. PTERIN_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Mu50 / ATCC 700699.

    Entry informationi

    Entry nameiDHPS_STAAM
    AccessioniPrimary (citable) accession number: P64141
    Secondary accession number(s): Q99W89
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3