Reviewed, UniProtKB/Swiss-Prot P64019 (ECH14_MYCBO)

Names and origin

Protein names

Recommended name:
    Probable enoyl-CoA hydratase echA14
    EC=4.2.1.17

Gene names

Name:	echA14
Ordered Locus Names:	Mb2511

Organism

Mycobacterium bovis [Complete proteome] [HAMAP]

Taxonomic identifier

1765 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Could possibly oxidize fatty acids using specific components By similarity.
Catalytic activity	(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.
Induction	Induced in response to the thiol oxidant diamide. Ref.2
Sequence similarities	Belongs to the enoyl-CoA hydratase/isomerase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	enoyl-CoA hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 256	256	Probable enoyl-CoA hydratase echA14		PRO_0000109342

Sequences

	Sequence		Length	Mass (Da)
	P64019-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: EB314ABF51113AED	FASTA	256	26,280
	10 20 30 40 50 60 MAQYDPVLLS VDKHVALITV NDPDRRNAVT DEMSAQLRAA IQRAEGDPDV HAVVVTGAGK 70 80 90 100 110 120 AFCAGADLSA LGAGVGDPAE PRLLRLYDGF MAVSSCNLPT IAAVNGAAVG AGLNLALAAD 130 140 150 160 170 180 VRIAGPAALF DARFQKLGLH PGGGATWMLQ RAVGPQVARA ALLFGMCFDA ESAVRHGLAL 190 200 210 220 230 240 MVADDPVTAA LELAAGPAAA PREVVLASKA TMRATASPGS LDLEQHELAK RLELGPQAKS 250 VQSPEFAARL AAAQHR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed: 12788972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.
[2]	"Thiol specific oxidative stress response in Mycobacteria." Dosanjh N.S., Rawat M., Chung J.-H., Av-Gay Y. FEMS Microbiol. Lett. 249:87-94(2005) [PubMed: 16006064] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INDUCTION. Strain: BCG / Pasteur.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX248342 Genomic DNA. Translation: CAD97372.1.
RefSeq	NP_856158.1.
3D structure databases
HSSP	HSSP built from PDB template 1MJ3 based on UniProtKB P14604.
ModBase	Search...
Genome annotation databases
GeneID	1094201.
GenomeReviews	Gene locus Mb2511 in contig BX248333_GR.
KEGG	mbo:Mb2511.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P64019.
OMA	LITINDP.
Enzyme and pathway databases
BRENDA	4.2.1.17. 3091.
Family and domain databases
InterPro	IPR001753. Crotonase_core. IPR018376. Enoyl-CoA_hyd/isom_CS. [Graphical view]
Pfam	PF00378. ECH. 1 hit. [Graphical view]
PROSITE	PS00166. ENOYL_COA_HYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ECH14_MYCBO

Accession

Primary (citable) accession number: P64019
Secondary accession number(s): O53211

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)