Reviewed, UniProtKB/Swiss-Prot P64017 (ECHA8_MYCBO)

Names and origin

Protein names

Recommended name:
    Probable enoyl-CoA hydratase echA8
    EC=4.2.1.17

Gene names

Name:	echA8
Ordered Locus Names:	Mb1099c

Organism

Mycobacterium bovis [Complete proteome] [HAMAP]

Taxonomic identifier

1765 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Could possibly oxidize fatty acids using specific components By similarity.
Catalytic activity	(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.
Sequence similarities	Belongs to the enoyl-CoA hydratase/isomerase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	enoyl-CoA hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 257	257	Probable enoyl-CoA hydratase echA8		PRO_0000109336

Sequences

	Sequence		Length	Mass (Da)
	P64017-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: C8907716969540AA	FASTA	257	27,273
	10 20 30 40 50 60 MTYETILVER DQRVGIITLN RPQALNALNS QVMNEVTSAA TELDDDPDIG AIIITGSAKA 70 80 90 100 110 120 FAAGADIKEM ADLTFADAFT ADFFATWGKL AAVRTPTIAA VAGYALGGGC ELAMMCDVLI 130 140 150 160 170 180 AADTAKFGQP EIKLGVLPGM GGSQRLTRAI GKAKAMDLIL TGRTMDAAEA ERSGLVSRVV 190 200 210 220 230 240 PADDLLTEAR ATATTISQMS ASAARMAKEA VNRAFESSLS EGLLYERRLF HSAFATEDQS 250 EGMAAFIEKR APQFTHR

References

[1]

"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed: 12788972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX248337 Genomic DNA. Translation: CAD93959.1.
RefSeq	NP_854754.1.
3D structure databases
HSSP	HSSP built from PDB template 1MJ3 based on UniProtKB P14604.
ModBase	Search...
Genome annotation databases
GeneID	1092072.
GenomeReviews	Gene locus Mb1099c in contig BX248333_GR.
KEGG	mbo:Mb1099c.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P64017.
OMA	IKEMQNL.
Enzyme and pathway databases
BRENDA	4.2.1.17. 3091.
Family and domain databases
InterPro	IPR001753. Crotonase_core. IPR018376. Enoyl-CoA_hyd/isom_CS. [Graphical view]
Pfam	PF00378. ECH. 1 hit. [Graphical view]
PROSITE	PS00166. ENOYL_COA_HYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ECHA8_MYCBO

Accession

Primary (citable) accession number: P64017
Secondary accession number(s): O53418

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	November 3, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)