Reviewed, UniProtKB/Swiss-Prot P64016 (ECHA8_MYCTU)

Names and origin

Protein names

Recommended name:
    Probable enoyl-CoA hydratase echA8
    EC=4.2.1.17

Gene names

Name:	echA8
Ordered Locus Names:	Rv1070c, MT1100
ORF Names:	MTV017.23c

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Could possibly oxidize fatty acids using specific components By similarity.
Catalytic activity	(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.
Sequence similarities	Belongs to the enoyl-CoA hydratase/isomerase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Molecular function	Lyase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	enoyl-CoA hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 257

257

Probable enoyl-CoA hydratase echA8

PRO_0000109338

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

257

Helix Strand Turn

Details...

Beta strand

4 – 11

8

Beta strand

16 – 19

4

Helix

22 – 24

3

Helix

30 – 44

15

Beta strand

52 – 55

4

Helix

68 – 71

4

Helix

75 – 81

7

Turn

82 – 86

5

Beta strand

97 – 101

5

Helix

111 – 115

5

Beta strand

116 – 122

7

Helix

130 – 133

4

Helix

145 – 149

5

Helix

153 – 160

8

Helix

167 – 172

6

Beta strand

177 – 180

4

Helix

182 – 184

3

Helix

185 – 197

13

Helix

201 – 213

13

Turn

214 – 216

3

Helix

224 – 233

10

Helix

237 – 240

4

Helix

244 – 247

4

Sequences

	Sequence		Length	Mass (Da)
	P64016-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: C8907716969540AA	FASTA	257	27,273
	10 20 30 40 50 60 MTYETILVER DQRVGIITLN RPQALNALNS QVMNEVTSAA TELDDDPDIG AIIITGSAKA 70 80 90 100 110 120 FAAGADIKEM ADLTFADAFT ADFFATWGKL AAVRTPTIAA VAGYALGGGC ELAMMCDVLI 130 140 150 160 170 180 AADTAKFGQP EIKLGVLPGM GGSQRLTRAI GKAKAMDLIL TGRTMDAAEA ERSGLVSRVV 190 200 210 220 230 240 PADDLLTEAR ATATTISQMS ASAARMAKEA VNRAFESSLS EGLLYERRLF HSAFATEDQS 250 EGMAAFIEKR APQFTHR

References

[1]

"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.

[2]

"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.

Cross-references

Sequence databases

EMBLGenBankDDBJ

BX842575 Genomic DNA. Translation: CAA17186.1.
AE000516 Genomic DNA. Translation: AAK45356.1.

PIR

D70893.

RefSeq

NP_215586.1.
NP_335542.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3H81	X-ray	1.80	A/B/C	1-257	[»]

ModBase

Search...

Genome annotation databases

GeneID

887117.
925082.

GenomeReviews

Gene locus MT1100 in contig AE000516_GR.
Gene locus Rv1070c in contig AL123456_GR.

KEGG

mtc:MT1100.
mtu:Rv1070c.

TIGR

MT1100.

Organism-specific databases

TubercuList

Rv1070c.

Phylogenomic databases

HOGENOM

P64016.

OMA

IKEMQNL.

Enzyme and pathway databases

BRENDA

4.2.1.17. 809.

Family and domain databases

InterPro

IPR001753. Crotonase_core.
IPR018376. Enoyl-CoA_hyd/isom_CS.
[Graphical view]

Pfam

PF00378. ECH. 1 hit.
[Graphical view]

PROSITE

PS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ECHA8_MYCTU

Accession

Primary (citable) accession number: P64016
Secondary accession number(s): O53418

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)