Reviewed, UniProtKB/Swiss-Prot P64014 (ECHA6_MYCTU)

Names and origin

Protein names

Recommended name:
    Probable enoyl-CoA hydratase echA6
    EC=4.2.1.17

Gene names

Name:	echA6
Ordered Locus Names:	Rv0905, MT0928
ORF Names:	MTCY31.33

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Could possibly oxidize fatty acids using specific components By similarity.
Catalytic activity	(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.
Sequence similarities	Belongs to the enoyl-CoA hydratase/isomerase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Molecular function	Lyase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	enoyl-CoA hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 243

243

Probable enoyl-CoA hydratase echA6

PRO_0000109335

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

243

Helix Strand Turn

Details...

Beta strand

5 – 7

3

Beta strand

10 – 14

5

Helix

18 – 20

3

Helix

26 – 37

12

Beta strand

45 – 50

6

Helix

68 – 70

3

Helix

71 – 84

14

Helix

103 – 107

5

Beta strand

108 – 110

3

Beta strand

112 – 114

3

Helix

122 – 125

4

Helix

132 – 141

10

Helix

147 – 153

7

Helix

159 – 164

6

Helix

174 – 185

12

Helix

189 – 200

12

Helix

210 – 219

10

Helix

223 – 233

11

Sequences

	Sequence		Length	Mass (Da)
	P64014-1 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 23BD1153F0159991	FASTA	243	26,029
	10 20 30 40 50 60 MIGITQAEAV LTIELQRPER RNALNSQLVE ELTQAIRKAG DGSARAIVLT GQGTAFCAGA 70 80 90 100 110 120 DLSGDAFAAD YPDRLIELHK AMDASPMPVV GAINGPAIGA GLQLAMQCDL RVVAPDAFFQ 130 140 150 160 170 180 FPTSKYGLAL DNWSIRRLSS LVGHGRARAM LLSAEKLTAE IALHTGMANR IGTLADAQAW 190 200 210 220 230 240 AAEIARLAPL AIQHAKRVLN DDGAIEEAWP AHKELFDKAW GSQDVIEAQV ARMEKRPPKF QGA

References

[1]

"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.

[2]

"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.

Cross-references

Sequence databases

EMBLGenBankDDBJ

BX842574 Genomic DNA. Translation: CAA97380.1.
AE000516 Genomic DNA. Translation: AAK45175.1.

PIR

F70783.

RefSeq

NP_215420.1.
NP_335361.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3HE2	X-ray	2.30	A/B/C	1-243	[»]

ModBase

Search...

Genome annotation databases

GeneID

885825.
926243.

GenomeReviews

Gene locus MT0928 in contig AE000516_GR.
Gene locus Rv0905 in contig AL123456_GR.

KEGG

mtc:MT0928.
mtu:Rv0905.

TIGR

MT0928.

Organism-specific databases

TubercuList

Rv0905.

Phylogenomic databases

HOGENOM

P64014.

OMA

DGAYEEP.

Enzyme and pathway databases

BRENDA

4.2.1.17. 809.

Family and domain databases

InterPro

IPR001753. Crotonase_core.
IPR018376. Enoyl-CoA_hyd/isom_CS.
[Graphical view]

Pfam

PF00378. ECH. 1 hit.
[Graphical view]

PROSITE

PS00166. ENOYL_COA_HYDRATASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ECHA6_MYCTU

Accession

Primary (citable) accession number: P64014
Secondary accession number(s): Q10533

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	November 3, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)