Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P64004

- DUT_BRUME

UniProt

P64004 - DUT_BRUME

Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene

dut

Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.UniRule annotation

    Catalytic activityi

    dUTP + H2O = dUMP + diphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei89 – 891SubstrateUniRule annotation
    Binding sitei103 – 1031Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation

    GO - Molecular functioni

    1. dUTP diphosphatase activity Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. dUMP biosynthetic process Source: UniProtKB-UniPathway
    2. dUTP metabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Nucleotide metabolism

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciBMEL224914:GCJ0-368-MONOMER.
    UniPathwayiUPA00610; UER00666.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deoxyuridine 5'-triphosphate nucleotidohydrolaseUniRule annotation (EC:3.6.1.23UniRule annotation)
    Short name:
    dUTPaseUniRule annotation
    Alternative name(s):
    dUTP pyrophosphataseUniRule annotation
    Gene namesi
    Name:dutUniRule annotation
    Ordered Locus Names:BMEI0358
    OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
    Taxonomic identifieri224914 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000000419: Chromosome I

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 157157Deoxyuridine 5'-triphosphate nucleotidohydrolasePRO_0000182835Add
    BLAST

    Proteomic databases

    PRIDEiP64004.

    Interactioni

    Protein-protein interaction databases

    STRINGi224914.BMEI0358.

    Structurei

    3D structure databases

    ProteinModelPortaliP64004.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni76 – 783Substrate bindingUniRule annotation
    Regioni93 – 953Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the dUTPase family.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000028968.
    KOiK01520.
    OMAiEPMERIA.
    OrthoDBiEOG689HXK.

    Family and domain databases

    Gene3Di2.70.40.10. 1 hit.
    HAMAPiMF_00116. dUTPase_bact.
    InterProiIPR029054. dUTPase-like.
    IPR008180. dUTPase/dCTP_deaminase.
    IPR008181. dUTPase_1.
    [Graphical view]
    PfamiPF00692. dUTPase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51283. SSF51283. 1 hit.
    TIGRFAMsiTIGR00576. dut. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P64004-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTAASSSAPT LGIIRLEHAK GLDLPAYETA GSAGMDLRAA VAEDRQIVLL    50
    PGRRTLVPTG LILEIPQGYE VQIRPRSGLA FKNGITCLNT PGTIDSDYRG 100
    EVKVLLINLG DDDFRIERGM RIAQAVFAPV IQPKIEERAK ISETARGAGG 150
    FGSTGTA 157
    Length:157
    Mass (Da):16,660
    Last modified:October 11, 2004 - v1
    Checksum:i7C9BB78942CFADEF
    GO

    Sequence cautioni

    The sequence AAL51539.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL51539.1. Different initiation.
    PIRiAH3296.
    RefSeqiNP_539275.1. NC_003317.1.

    Genome annotation databases

    EnsemblBacteriaiAAL51539; AAL51539; BMEI0358.
    GeneIDi1196069.
    KEGGibme:BMEI0358.
    PATRICi17850746. VBIBruMel92729_0395.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008917 Genomic DNA. Translation: AAL51539.1 . Different initiation.
    PIRi AH3296.
    RefSeqi NP_539275.1. NC_003317.1.

    3D structure databases

    ProteinModelPortali P64004.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224914.BMEI0358.

    Proteomic databases

    PRIDEi P64004.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL51539 ; AAL51539 ; BMEI0358 .
    GeneIDi 1196069.
    KEGGi bme:BMEI0358.
    PATRICi 17850746. VBIBruMel92729_0395.

    Phylogenomic databases

    HOGENOMi HOG000028968.
    KOi K01520.
    OMAi EPMERIA.
    OrthoDBi EOG689HXK.

    Enzyme and pathway databases

    UniPathwayi UPA00610 ; UER00666 .
    BioCyci BMEL224914:GCJ0-368-MONOMER.

    Miscellaneous databases

    PROi P64004.

    Family and domain databases

    Gene3Di 2.70.40.10. 1 hit.
    HAMAPi MF_00116. dUTPase_bact.
    InterProi IPR029054. dUTPase-like.
    IPR008180. dUTPase/dCTP_deaminase.
    IPR008181. dUTPase_1.
    [Graphical view ]
    Pfami PF00692. dUTPase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51283. SSF51283. 1 hit.
    TIGRFAMsi TIGR00576. dut. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 16M / ATCC 23456 / NCTC 10094.

    Entry informationi

    Entry nameiDUT_BRUME
    AccessioniPrimary (citable) accession number: P64004
    Secondary accession number(s): Q8YIT4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Brucella melitensis
      Brucella melitensis (strain 16M): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3