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P64004

- DUT_BRUME

UniProt

P64004 - DUT_BRUME

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Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene
dut, BMEI0358
Organism
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity.UniRule annotation

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei89 – 891Substrate By similarity
Binding sitei103 – 1031Substrate; via amide nitrogen and carbonyl oxygen By similarity

GO - Molecular functioni

  1. dUTP diphosphatase activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. dUMP biosynthetic process Source: UniProtKB-UniPathway
  2. dUTP metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciBMEL224914:GCJ0-368-MONOMER.
UniPathwayiUPA00610; UER00666.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC:3.6.1.23)
Short name:
dUTPase
Alternative name(s):
dUTP pyrophosphatase
Gene namesi
Name:dut
Ordered Locus Names:BMEI0358
OrganismiBrucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Taxonomic identifieri224914 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000419: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 157157Deoxyuridine 5'-triphosphate nucleotidohydrolaseUniRule annotationPRO_0000182835Add
BLAST

Proteomic databases

PRIDEiP64004.

Interactioni

Protein-protein interaction databases

STRINGi224914.BMEI0358.

Structurei

3D structure databases

ProteinModelPortaliP64004.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni76 – 783Substrate binding By similarity
Regioni93 – 953Substrate binding By similarity

Sequence similaritiesi

Belongs to the dUTPase family.

Phylogenomic databases

HOGENOMiHOG000028968.
KOiK01520.
OMAiEPMERIA.
OrthoDBiEOG689HXK.

Family and domain databases

Gene3Di2.70.40.10. 1 hit.
HAMAPiMF_00116. dUTPase_bact.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR00576. dut. 1 hit.

Sequencei

Sequence statusi: Complete.

P64004-1 [UniParc]FASTAAdd to Basket

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MTAASSSAPT LGIIRLEHAK GLDLPAYETA GSAGMDLRAA VAEDRQIVLL    50
PGRRTLVPTG LILEIPQGYE VQIRPRSGLA FKNGITCLNT PGTIDSDYRG 100
EVKVLLINLG DDDFRIERGM RIAQAVFAPV IQPKIEERAK ISETARGAGG 150
FGSTGTA 157
Length:157
Mass (Da):16,660
Last modified:October 11, 2004 - v1
Checksum:i7C9BB78942CFADEF
GO

Sequence cautioni

The sequence AAL51539.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008917 Genomic DNA. Translation: AAL51539.1. Different initiation.
PIRiAH3296.
RefSeqiNP_539275.1. NC_003317.1.

Genome annotation databases

EnsemblBacteriaiAAL51539; AAL51539; BMEI0358.
GeneIDi1196069.
KEGGibme:BMEI0358.
PATRICi17850746. VBIBruMel92729_0395.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008917 Genomic DNA. Translation: AAL51539.1 . Different initiation.
PIRi AH3296.
RefSeqi NP_539275.1. NC_003317.1.

3D structure databases

ProteinModelPortali P64004.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224914.BMEI0358.

Proteomic databases

PRIDEi P64004.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL51539 ; AAL51539 ; BMEI0358 .
GeneIDi 1196069.
KEGGi bme:BMEI0358.
PATRICi 17850746. VBIBruMel92729_0395.

Phylogenomic databases

HOGENOMi HOG000028968.
KOi K01520.
OMAi EPMERIA.
OrthoDBi EOG689HXK.

Enzyme and pathway databases

UniPathwayi UPA00610 ; UER00666 .
BioCyci BMEL224914:GCJ0-368-MONOMER.

Miscellaneous databases

PROi P64004.

Family and domain databases

Gene3Di 2.70.40.10. 1 hit.
HAMAPi MF_00116. dUTPase_bact.
InterProi IPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
[Graphical view ]
Pfami PF00692. dUTPase. 1 hit.
[Graphical view ]
SUPFAMi SSF51283. SSF51283. 1 hit.
TIGRFAMsi TIGR00576. dut. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 16M / ATCC 23456 / NCTC 10094.

Entry informationi

Entry nameiDUT_BRUME
AccessioniPrimary (citable) accession number: P64004
Secondary accession number(s): Q8YIT4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: June 11, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella melitensis
    Brucella melitensis (strain 16M): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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