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Protein

DNA polymerase IV

Gene

dinB

Organism
Staphylococcus aureus (strain N315)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII.UniRule annotation

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi10 – 101MagnesiumUniRule annotation
Sitei15 – 151Substrate discriminationUniRule annotation
Metal bindingi105 – 1051MagnesiumUniRule annotation
Active sitei106 – 1061UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Mutator protein, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciSAUR158879:GJCB-1823-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase IVUniRule annotation (EC:2.7.7.7UniRule annotation)
Short name:
Pol IVUniRule annotation
Gene namesi
Name:dinBUniRule annotation
Ordered Locus Names:SA1711
OrganismiStaphylococcus aureus (strain N315)
Taxonomic identifieri158879 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000751 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 356356DNA polymerase IVPRO_0000173945Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP63992.
SMRiP63992. Positions 6-342.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 187182UmuCUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the DNA polymerase type-Y family.UniRule annotation
Contains 1 umuC domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000082707.
OMAiMQIREIF.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
HAMAPiMF_01113. DNApol_IV. 1 hit.
InterProiIPR017961. DNA_pol_Y-fam_little_finger.
IPR022880. DNApol_IV.
IPR001126. UmuC.
[Graphical view]
PfamiPF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiPS50173. UMUC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P63992-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTERRIIHID MDYFFAQVEM RDNPKLKGKP VIVGGKASSR GVVSTASYEA
60 70 80 90 100
RKYGVHSAMP MSQAHKLCPN GYFVTSNFGA YRETSAQIMS IFRSYTDKVE
110 120 130 140 150
PMSLDEAYLD ITELVRPDLP ASKIAQYIRK DILEQTHLTA SAGVSYNKFL
160 170 180 190 200
AKLASGMNKP DGLTVIDYQN VHDILMTLDI GDFPGVGKAS KKVMHDNGIF
210 220 230 240 250
NGRDLYEKTE FELIRLFGKR GRGLYNKARG IDHSEVKSSR VRKSVGTERT
260 270 280 290 300
FATDVNDDEE ILRKVWELSG KTAERLNKLQ KSAKTVTVKI KTYQFETLSK
310 320 330 340 350
QMSLRDSVSS EEDIYNIAYL LYNDLKDPDV PIRLIGVTVG NLEQSTYKNM

TIYDFI
Length:356
Mass (Da):40,297
Last modified:October 11, 2004 - v1
Checksum:iAFE53A4384825272
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000018 Genomic DNA. Translation: BAB42981.1.
PIRiF89977.
RefSeqiWP_000140172.1. NC_002745.2.

Genome annotation databases

EnsemblBacteriaiBAB42981; BAB42981; BAB42981.
PATRICi19575826. VBIStaAur116463_1845.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000018 Genomic DNA. Translation: BAB42981.1.
PIRiF89977.
RefSeqiWP_000140172.1. NC_002745.2.

3D structure databases

ProteinModelPortaliP63992.
SMRiP63992. Positions 6-342.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB42981; BAB42981; BAB42981.
PATRICi19575826. VBIStaAur116463_1845.

Phylogenomic databases

HOGENOMiHOG000082707.
OMAiMQIREIF.

Enzyme and pathway databases

BioCyciSAUR158879:GJCB-1823-MONOMER.

Family and domain databases

Gene3Di3.30.1490.100. 1 hit.
HAMAPiMF_01113. DNApol_IV. 1 hit.
InterProiIPR017961. DNA_pol_Y-fam_little_finger.
IPR022880. DNApol_IV.
IPR001126. UmuC.
[Graphical view]
PfamiPF00817. IMS. 1 hit.
PF11799. IMS_C. 1 hit.
[Graphical view]
SUPFAMiSSF100879. SSF100879. 1 hit.
PROSITEiPS50173. UMUC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDPO4_STAAN
AccessioniPrimary (citable) accession number: P63992
Secondary accession number(s): Q99SZ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: September 7, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.