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Protein

DNA polymerase III subunit alpha

Gene

dnaE

Organism
Staphylococcus aureus (strain N315)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase (By similarity).By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase III subunit alpha (EC:2.7.7.7)
Gene namesi
Name:dnaE
Ordered Locus Names:SA1525
OrganismiStaphylococcus aureus (strain N315)
Taxonomic identifieri158879 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000751 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001033411 – 1065DNA polymerase III subunit alphaAdd BLAST1065

Proteomic databases

PRIDEiP63980.

Interactioni

Subunit structurei

DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the PolIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP63980.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000021785.
KOiK02337.
OMAiQIMLNSA.

Family and domain databases

InterProiIPR011708. DNA_pol3_alpha.
IPR029460. DNAPol_HHH.
IPR004365. NA-bd_OB_tRNA.
IPR004013. PHP_dom.
IPR003141. Pol/His_phosphatase_N.
IPR016195. Pol/histidinol_Pase-like.
IPR004805. PolC_alpha.
[Graphical view]
PfamiPF07733. DNA_pol3_alpha. 1 hit.
PF14579. HHH_6. 1 hit.
PF02811. PHP. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
SMARTiSM00481. POLIIIAc. 1 hit.
[Graphical view]
SUPFAMiSSF89550. SSF89550. 1 hit.
TIGRFAMsiTIGR00594. polc. 1 hit.

Sequencei

Sequence statusi: Complete.

P63980-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAYLNIHTA YDLLNSSLKI EDAVRLAVSE NVDALAITDT NVLYGFPKFY
60 70 80 90 100
DACIANNIKP IFGMTIYVTN GLNTVETVVL AKNNDGLKDL YQLSSEIKMN
110 120 130 140 150
SMENVSFELL QQFSSNLIII FKNVADEHRD IVQVFDSHED TYLDHQSVLV
160 170 180 190 200
QGIKHVWIQN VCYQTRQDAD TISALAAIRD NAKLDLIHDQ EDFGAHFLTE
210 220 230 240 250
KEIKQLDINQ EYLTQVDVIA QKCNAELKYH QSLLPQYQTP NDESAKKYLW
260 270 280 290 300
RVLVTQLKKL ELNYDVYLER LKYEYKVITN MGFEDYFLIV SDLIHYAKTN
310 320 330 340 350
DVMVGPGRGS SAGSLVSYLL GITTIDPIKF NLLFERFLNP ERVTMPDIDI
360 370 380 390 400
DFEDTRRERV IQYVQEKYGE LHVSGIVTFG HLLARAVARD VGRIMGFDEV
410 420 430 440 450
TLNEISSLIP HKLGITLDEA YQIDDFKKFV HRNHRHERWF SICKKLEGLP
460 470 480 490 500
RHTSTHAAGI IINDHPLYEY APLTKGDTGL LTQWTMTEAE RIGLLKIDFL
510 520 530 540 550
GLRNLSIIHQ ILIQVKKDLG INIDIEKIPF DDQKVFELLS QGDTTGIFQL
560 570 580 590 600
ESDGVRSVLK KLKPEHFEDI VAVTSLYRPG PMEEIPTYIT RRHDPSKVQY
610 620 630 640 650
LHPHLEPILK NTYGVIIYQE QIMQIASTFA NFSYGEADIL RRAMSKKNRA
660 670 680 690 700
VLESERQHFI EGAKQNGYHE DISKQIFDLI LKFADYGFPR AHAVSYSKIA
710 720 730 740 750
YIMSFLKVHY PNYFYANILS NVIGSEKKTA QMIEEAKKQG ITILPPNINE
760 770 780 790 800
SHWFYKPSQE GIYLSIGTIK GVGYQSVKVI VDERYQNGKF KDFFDFARRI
810 820 830 840 850
PKRVKTRKLL EALILVGAFD AFGKTRSTLL QAIDQVLDGD LNIEQDGFLF
860 870 880 890 900
DILTPKQMYE DKEELPDALI SQYEKEYLGF YVSQHPVDKK FVAKQYLTIF
910 920 930 940 950
KLSNAQNYKP ILVQFDKVKQ IRTKNGQNMA FVTLNDGIET LDGVIFPNQF
960 970 980 990 1000
KKYEELLSHN DLFIVSGKFD HRKQQRQLII NEIQTLATFE EQKLAFAKQI
1010 1020 1030 1040 1050
IIRNKSQIDM FEEMIKATKE NANDVVLSFY DETIKQMTTL GYINQKDSMF
1060
NNFIQSFNPS DIRLI
Length:1,065
Mass (Da):122,836
Last modified:October 11, 2004 - v1
Checksum:iEB23C016E20AB196
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000018 Genomic DNA. Translation: BAB42792.1.
PIRiC89954.
RefSeqiWP_000226919.1. NC_002745.2.

Genome annotation databases

EnsemblBacteriaiBAB42792; BAB42792; BAB42792.
KEGGisau:SA1525.
PATRICi19575350. VBIStaAur116463_1645.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000018 Genomic DNA. Translation: BAB42792.1.
PIRiC89954.
RefSeqiWP_000226919.1. NC_002745.2.

3D structure databases

ProteinModelPortaliP63980.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP63980.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB42792; BAB42792; BAB42792.
KEGGisau:SA1525.
PATRICi19575350. VBIStaAur116463_1645.

Phylogenomic databases

HOGENOMiHOG000021785.
KOiK02337.
OMAiQIMLNSA.

Family and domain databases

InterProiIPR011708. DNA_pol3_alpha.
IPR029460. DNAPol_HHH.
IPR004365. NA-bd_OB_tRNA.
IPR004013. PHP_dom.
IPR003141. Pol/His_phosphatase_N.
IPR016195. Pol/histidinol_Pase-like.
IPR004805. PolC_alpha.
[Graphical view]
PfamiPF07733. DNA_pol3_alpha. 1 hit.
PF14579. HHH_6. 1 hit.
PF02811. PHP. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
SMARTiSM00481. POLIIIAc. 1 hit.
[Graphical view]
SUPFAMiSSF89550. SSF89550. 1 hit.
TIGRFAMsiTIGR00594. polc. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDPO3A_STAAN
AccessioniPrimary (citable) accession number: P63980
Secondary accession number(s): Q99TG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 11, 2004
Last modified: November 2, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.