P63978 (DPO3A_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase III subunit alpha
EC=2.7.7.7

Gene names

Name:	dnaE
Synonyms:	dnaE1
Ordered Locus Names:	Mb1574

Organism

Mycobacterium bovis [Complete proteome] [HAMAP]

Taxonomic identifier

1765 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	1184 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subunit structure	DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the PolIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE subfamily.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1184	1184	DNA polymerase III subunit alpha		PRO_0000103330

Sequences

	Sequence		Length	Mass (Da)
	P63978 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: A87AB7F0F2E08909	FASTA	1,184	129,323
	10 20 30 40 50 60 MSGSSAGSSF VHLHNHTEYS MLDGAAKITP MLAEVERLGM PAVGMTDHGN MFGASEFYNS 70 80 90 100 110 120 ATKAGIKPII GVEAYIAPGS RFDTRRILWG DPSQKADDVS GSGSYTHLTM MAENATGLRN 130 140 150 160 170 180 LFKLSSHASF EGQLSKWSRM DAELIAEHAE GIIITTGCPS GEVQTRLRLG QDREALEAAA 190 200 210 220 230 240 KWREIVGPDN YFLELMDHGL TIERRVRDGL LEIGRALNIP PLATNDCHYV TRDAAHNHEA 250 260 270 280 290 300 LLCVQTGKTL SDPNRFKFDG DGYYLKSAAE MRQIWDDEVP GACDSTLLIA ERVQSYADVW 310 320 330 340 350 360 TPRDRMPVFP VPDGHDQASW LRHEVDAGLR RRFPAGPPDG YRERAAYEID VICSKGFPSY 370 380 390 400 410 420 FLIVADLISY ARSAGIRVGP GRGSAAGSLV AYALGITDID PIPHGLLFER FLNPERTSMP 430 440 450 460 470 480 DIDIDFDDRR RGEMVRYAAD KWGHDRVAQV ITFGTIKTKA ALKDSARIHY GQPGFAIADR 490 500 510 520 530 540 ITKALPPAIM AKDIPLSGIT DPSHERYKEA AEVRGLIETD PDVRTIYQTA RGLEGLIRNA 550 560 570 580 590 600 GVHACAVIMS SEPLTEAIPL WKRPQDGAII TGWDYPACEA IGLLKMDFLG LRNLTIIGDA 610 620 630 640 650 660 IDNVRANRGI DLDLESVPLD DKATYELLGR GDTLGVFQLD GGPMRDLLRR MQPTGFEDVV 670 680 690 700 710 720 AVIALYRPGP MGMNAHNDYA DRKNNRQAIK PIHPELEEPL REILAETYGL IVYQEQIMRI 730 740 750 760 770 780 AQKVASYSLA RADILRKAMG KKKREVLEKE FEGFSDGMQA NGFSPAAIKA LWDTILPFAD 790 800 810 820 830 840 YAFNKSHAAG YGMVSYWTAY LKANYPAEYM AGLLTSVGDD KDKAAVYLAD CRKLGITVLP 850 860 870 880 890 900 PDVNESGLNF ASVGQDIRYG LGAVRNVGAN VVGSLLQTRN DKGKFTDFSD YLNKIDISAC 910 920 930 940 950 960 NKKVTESLIK AGAFDSLGHA RKGLFLVHSD AVDSVLGTKK AEALGQFDLF GSNDDGTGTA 970 980 990 1000 1010 1020 DPVFTIKVPD DEWEDKHKLA LEREMLGLYV SGHPLNGVAH LLAAQVDTAI PAILDGDVPN 1030 1040 1050 1060 1070 1080 DAQVRVGGIL ASVNRRVNKN GMPWASAQLE DLTGGIEVMF FPHTYSSYGA DIVDDAVVLV 1090 1100 1110 1120 1130 1140 NAKVAVRDDR IALIANDLTV PDFSNAEVER PLAVSLPTRQ CTFDKVSALK QVLARHPGTS 1150 1160 1170 1180 QVHLRLISGD RITTLALDQS LRVTPSPALM GDLKELLGPG CLGS

References

[1]

"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed: 12788972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248339 Genomic DNA. Translation: CAD96241.1.
RefSeq	NP_855226.1. NC_002945.3.
3D structure databases
ProteinModelPortal	P63978.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000015260; EBMYCP00000015095; EBMYCG00000015257.
GeneID	1092442.
GenomeReviews	Gene locus Mb1574 in contig BX248333_GR.
KEGG	mbo:Mb1574.
PATRIC	18005147. VBIMycBov88188_1720.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016568.
HOGENOM	HBG734490.
OMA	RVLQMPY.
ProtClustDB	PRK05673.
Enzyme and pathway databases
BioCyc	MBOV233413:MB1574-MONOMER.
Family and domain databases
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA-bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR016195. Pol/histidinol_Pase-like. IPR004805. PolC_alpha. [Graphical view]
KO	K02337.
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
SUPFAM	SSF89550. PHP-like. 1 hit.
TIGRFAMs	TIGR00594. Polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DPO3A_MYCBO

Accession

Primary (citable) accession number: P63978
Secondary accession number(s): Q10779

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program