P63971 (DNAJ_STAAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chaperone protein DnaJ

Gene names

Name:	dnaJ
Ordered Locus Names:	SA1408

Organism

Staphylococcus aureus (strain N315) [Complete proteome] [HAMAP]

Taxonomic identifier

158879 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins By similarity. HAMAP MF_01152
Cofactor	Binds 2 zinc ions per monomer By similarity. HAMAP MF_01152
Subunit structure	Homodimer By similarity. HAMAP MF_01152
Subcellular location	Cytoplasm By similarity HAMAP MF_01152.
Domain	The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity By similarity. HAMAP MF_01152
Sequence similarities	Belongs to the DnaJ family. Contains 1 CR-type zinc finger. Contains 1 J domain.

Ontologies

Keywords
Biological process	DNA replication Stress response
Cellular component	Cytoplasm
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW protein folding Inferred from electronic annotation. Source: InterPro response to heat Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro heat shock protein binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW unfolded protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	Chaperone protein DnaJ HAMAP MF_01152		PRO_0000070884
Regions
	Domain	5 – 69	65	J
	Repeat	149 – 156	8	CXXCXGXG motif HAMAP MF_01152
	Repeat	166 – 173	8	CXXCXGXG motif HAMAP MF_01152
	Repeat	192 – 199	8	CXXCXGXG motif HAMAP MF_01152
	Repeat	206 – 213	8	CXXCXGXG motif HAMAP MF_01152
	Zinc finger	136 – 218	83	CR-type HAMAP MF_01152
	Compositional bias	74 – 119	46	Gly-rich HAMAP MF_01152
Sites
	Metal binding	149	1	Zinc 1 By similarity
	Metal binding	152	1	Zinc 1 By similarity
	Metal binding	166	1	Zinc 2 By similarity
	Metal binding	169	1	Zinc 2 By similarity
	Metal binding	192	1	Zinc 2 By similarity
	Metal binding	195	1	Zinc 2 By similarity
	Metal binding	206	1	Zinc 1 By similarity
	Metal binding	209	1	Zinc 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P63971 [UniParc]. Last modified October 11, 2004. Version 1. Checksum: 1D6C22CBFF511E5B	FASTA	379	41,761
	10 20 30 40 50 60 MAKRDYYEVL GISKDASKDE IKKAYRKLSK KYHPDINKEE GADEKFKEIS EAYEVLSDDN 70 80 90 100 110 120 KRASYDQFGH DGPQGFGGQG FNGSDFGGFS GFGGGGFEDI FSSFFGGGRQ RDPNAPQKGD 130 140 150 160 170 180 DLQYTMTLTF EEAVFGTTKE ISIRKDVTCE TCHGDGAKPG TSKKTCSYCN GAGHVAVEQN 190 200 210 220 230 240 TILGRVRTEQ VCPKCNGSGQ EFEEACPTCH GKGTENKTVK LEVKVPEGVD NEQQIRLAGE 250 260 270 280 290 300 GSPGVNGGPA GDLYVVFRVK PSETFKRDGD DIYYKLNVSF PQAALGDEIK IPTLNNEVML 310 320 330 340 350 360 TIPAGTQTGK QFRLKEKGIK NVHGYGYGDL YVDIKVVTPT KLTDRQKELM KEFAQLNGEE 370 INEQPSNFKD RAKRFFKGE

References

[1]	"Whole genome sequencing of meticillin-resistant Staphylococcus aureus." Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L., Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M., Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y. , Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H., Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K., Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H., Hiramatsu K. Lancet 357:1225-1240(2001) [PubMed: 11418146] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: N315.
[2]	"Shotgun proteomic analysis of total and membrane protein extracts of S. aureus strain N315." Vaezzadeh A.R., Deshusses J., Lescuyer P., Hochstrasser D.F. Submitted (OCT-2007) to UniProtKB Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: N315.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000018 Genomic DNA. Translation: BAB42671.1.
PIR	B89939.
RefSeq	NP_374692.1. NC_002745.2.
3D structure databases
ProteinModelPortal	P63971.
SMR	P63971. Positions 2-70.
ModBase	Search...
Protein-protein interaction databases
STRING	P63971.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000000617; EBSTAP00000000617; EBSTAG00000000617.
GeneID	1124249.
GenomeReviews	Gene locus SA1408 in contig BA000018_GR.
KEGG	sau:SA1408.
PATRIC	19575090. VBIStaAur116463_1514.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0484.
GeneTree	EBGT00050000024869.
HOGENOM	HBG635315.
OMA	ENKRANY.
PhylomeDB	P63971.
ProtClustDB	PRK14280.
Enzyme and pathway databases
BioCyc	SAUR158879:SA1408-MONOMER.
Family and domain databases
HAMAP	MF_01152. DnaJ. [Tree]
InterPro	IPR012724. DnaJ. IPR002939. DnaJ_C. IPR001623. DnaJ_N. IPR018253. Heat_shock_DnaJ_CS. IPR008971. HSP40/DnaJ_pept-bd. IPR003095. Hsp_DnaJ. IPR001305. HSP_DnaJ_Cys-rich_dom. [Graphical view]
Gene3D	G3DSA:1.10.287.110. DnaJ_N. 1 hit. G3DSA:2.10.230.10. HSP_DnaJ_cys-rich. 1 hit.
KO	K03686.
PANTHER	PTHR24076:SF80. PTHR24076:SF80. 1 hit.
Pfam	PF00226. DnaJ. 1 hit. PF01556. DnaJ_C. 1 hit. PF00684. DnaJ_CXXCXGXG. 1 hit. [Graphical view]
PRINTS	PR00625. JDOMAIN.
SMART	SM00271. DnaJ. 1 hit. [Graphical view]
SUPFAM	SSF46565. DnaJ_N. 1 hit. SSF49493. HSP40_DnaJ_pep. 2 hits. SSF57938. HSP_DnaJ_cys-rich. 1 hit.
TIGRFAMs	TIGR02349. DnaJ_bact. 1 hit.
PROSITE	PS00636. DNAJ_1. 1 hit. PS50076. DNAJ_2. 1 hit. PS51188. ZF_CR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAJ_STAAN

Accession

Primary (citable) accession number: P63971
Secondary accession number(s): Q99TR8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	October 11, 2004
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program