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P63948

- DAPA_STAAN

UniProt

P63948 - DAPA_STAAN

Protein

4-hydroxy-tetrahydrodipicolinate synthase

Gene

dapA

Organism
Staphylococcus aureus (strain N315)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (11 Oct 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).UniRule annotation

    Catalytic activityi

    Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H2O.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei46 – 461Part of a proton relay during catalysisUniRule annotation
    Binding sitei47 – 471PyruvateUniRule annotation
    Sitei109 – 1091Part of a proton relay during catalysisUniRule annotation
    Active sitei135 – 1351Proton donor/acceptorUniRule annotation
    Active sitei163 – 1631Schiff-base intermediate with substrateUniRule annotation
    Binding sitei206 – 2061Pyruvate; via carbonyl oxygenUniRule annotation

    GO - Molecular functioni

    1. 4-hydroxy-tetrahydrodipicolinate synthase Source: UniProtKB-EC
    2. amine-lyase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. diaminopimelate biosynthetic process Source: UniProtKB-HAMAP
    2. lysine biosynthetic process via diaminopimelate Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Amino-acid biosynthesis, Diaminopimelate biosynthesis, Lysine biosynthesis

    Keywords - Ligandi

    Schiff base

    Enzyme and pathway databases

    BioCyciSAUR158879:GJCB-1294-MONOMER.
    UniPathwayiUPA00034; UER00017.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-hydroxy-tetrahydrodipicolinate synthaseUniRule annotation (EC:4.3.3.7UniRule annotation)
    Short name:
    HTPA synthaseUniRule annotation
    Gene namesi
    Name:dapAUniRule annotation
    Ordered Locus Names:SA1227
    OrganismiStaphylococcus aureus (strain N315)
    Taxonomic identifieri158879 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000000751: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2952954-hydroxy-tetrahydrodipicolinate synthasePRO_0000103156Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi158879.SA1227.

    Structurei

    3D structure databases

    ProteinModelPortaliP63948.
    SMRiP63948. Positions 2-293.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DapA family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0329.
    HOGENOMiHOG000173604.
    KOiK01714.
    OMAiLADHENI.
    OrthoDBiEOG6W7235.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00418. DapA.
    InterProiIPR013785. Aldolase_TIM.
    IPR005263. DapA.
    IPR002220. DapA-like.
    IPR020625. Dihydrodipicolinate_synth_AS.
    [Graphical view]
    PANTHERiPTHR12128. PTHR12128. 1 hit.
    PfamiPF00701. DHDPS. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001365. DHDPS. 1 hit.
    PRINTSiPR00146. DHPICSNTHASE.
    TIGRFAMsiTIGR00674. dapA. 1 hit.
    PROSITEiPS00666. DHDPS_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P63948-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTHLFEGVGV ALTTPFTNNK VNLEALKAHV NFLLENNAQA IIVNGTTAES    50
    PTLTTDEKER ILKTVIDLVD KRVPVIAGTG TNDTEKSIQA SFQAKALGAD 100
    AIMLITPYYN KTNQRGLVKH FEAITDAVKL PVVLYNVPSR TNMTIEPETV 150
    EILSQHPYIV ALKDATNDFE YLEEVKKRID TNSFALYSGN DDNVVEYYQR 200
    GGQGVISVIA NVIPKEFQAL YDAQQSGLDI QDQFKPIGTL LSALSVDINP 250
    IPIKALTSYL EFGNYELRLP LVSLEDTDTK VLREAYDTFK AGENE 295
    Length:295
    Mass (Da):32,660
    Last modified:October 11, 2004 - v1
    Checksum:iEAD1E6DD4CE05A17
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000018 Genomic DNA. Translation: BAB42487.1.
    PIRiC89916.
    RefSeqiNP_374508.1. NC_002745.2.

    Genome annotation databases

    EnsemblBacteriaiBAB42487; BAB42487; BAB42487.
    GeneIDi1124066.
    KEGGisau:SA1227.
    PATRICi19574704. VBIStaAur116463_1321.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000018 Genomic DNA. Translation: BAB42487.1 .
    PIRi C89916.
    RefSeqi NP_374508.1. NC_002745.2.

    3D structure databases

    ProteinModelPortali P63948.
    SMRi P63948. Positions 2-293.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 158879.SA1227.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB42487 ; BAB42487 ; BAB42487 .
    GeneIDi 1124066.
    KEGGi sau:SA1227.
    PATRICi 19574704. VBIStaAur116463_1321.

    Phylogenomic databases

    eggNOGi COG0329.
    HOGENOMi HOG000173604.
    KOi K01714.
    OMAi LADHENI.
    OrthoDBi EOG6W7235.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00017 .
    BioCyci SAUR158879:GJCB-1294-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00418. DapA.
    InterProi IPR013785. Aldolase_TIM.
    IPR005263. DapA.
    IPR002220. DapA-like.
    IPR020625. Dihydrodipicolinate_synth_AS.
    [Graphical view ]
    PANTHERi PTHR12128. PTHR12128. 1 hit.
    Pfami PF00701. DHDPS. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001365. DHDPS. 1 hit.
    PRINTSi PR00146. DHPICSNTHASE.
    TIGRFAMsi TIGR00674. dapA. 1 hit.
    PROSITEi PS00666. DHDPS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: N315.

    Entry informationi

    Entry nameiDAPA_STAAN
    AccessioniPrimary (citable) accession number: P63948
    Secondary accession number(s): Q99U89
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.Curated

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3